Production and purification of the PEAK pseudokinases for structural and functional studies.
Dimerization
Oligomerization
PEAK pseudokinases
Protein expression
Protein purification
Scaffolds
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
7
5
2022
pubmed:
8
5
2022
medline:
11
5
2022
Statut:
ppublish
Résumé
The PEAK family of pseudokinases, which comprises PEAK1, PEAK2 and PEAK3, are newly identified scaffolds that dynamically assemble oncogenic signaling pathways known to contribute to the development of several aggressive cancers. A striking feature of this unique family of pseudokinase scaffolds is their large multi-domain structure, which allows them to achieve protein complex assemblies through their structural plasticity and functional versatility. Recent structural advances have begun to reveal the critical regulatory elements that control their function. Specifically, the dimer-dependent scaffolding activity of PEAK pseudokinases is emerging as a critical mechanism for their signaling function, in addition to their ability to hetero-associate to form higher-order regulatory networks to diversify and amplify their signaling output. Here, we present a suite of techniques that enable the efficient expression and purification of PEAK proteins for functional characterization.
Identifiants
pubmed: 35525538
pii: S0076-6879(22)00113-6
doi: 10.1016/bs.mie.2022.03.022
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-35Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.