A novel gene from the acidophilic bacterium Leptospirillum sp. CF-1 and its role in oxidative stress and chromate tolerance.
Chromate
Hydrogen peroxide
Hypothetical proteins
Leptospirillum
Och operon
Oxidative stress
Journal
Biological research
ISSN: 0717-6287
Titre abrégé: Biol Res
Pays: England
ID NLM: 9308271
Informations de publication
Date de publication:
07 May 2022
07 May 2022
Historique:
received:
27
09
2021
accepted:
15
04
2022
entrez:
7
5
2022
pubmed:
8
5
2022
medline:
11
5
2022
Statut:
epublish
Résumé
Acidophilic microorganisms like Leptospirillum sp. CF-1 thrive in environments with extremely low pH and high concentrations of dissolved heavy metals that can induce the generation of reactive oxygen species (ROS). Several hypothetical genes and proteins from Leptospirillum sp. CF-1 are known to be up-regulated under oxidative stress conditions. In the present work, the function of hypothetical gene ABH19_09590 from Leptospirillum sp. CF-1 was studied. Heterologous expression of this gene in Escherichia coli led to an increase in the ability to grow under oxidant conditions with 5 mM K Altogether, these results suggest that the och operon plays a protective role against chromate and hydrogen peroxide and is an important mechanism required to face polyextremophilic conditions in acid environments.
Sections du résumé
BACKGROUND
BACKGROUND
Acidophilic microorganisms like Leptospirillum sp. CF-1 thrive in environments with extremely low pH and high concentrations of dissolved heavy metals that can induce the generation of reactive oxygen species (ROS). Several hypothetical genes and proteins from Leptospirillum sp. CF-1 are known to be up-regulated under oxidative stress conditions.
RESULTS
RESULTS
In the present work, the function of hypothetical gene ABH19_09590 from Leptospirillum sp. CF-1 was studied. Heterologous expression of this gene in Escherichia coli led to an increase in the ability to grow under oxidant conditions with 5 mM K
CONCLUSIONS
CONCLUSIONS
Altogether, these results suggest that the och operon plays a protective role against chromate and hydrogen peroxide and is an important mechanism required to face polyextremophilic conditions in acid environments.
Identifiants
pubmed: 35525996
doi: 10.1186/s40659-022-00388-0
pii: 10.1186/s40659-022-00388-0
pmc: PMC9080137
doi:
Substances chimiques
Bacterial Proteins
0
Chromates
0
Reactive Oxygen Species
0
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19Subventions
Organisme : Fondo Nacional de Desarrollo Científico y Tecnológico
ID : 1170799
Organisme : Fondo Nacional de Desarrollo Científico y Tecnológico
ID : 1211386
Organisme : Departamento de Investigaciones Científicas y Tecnológicas, Universidad de Santiago de Chile
ID : Proyecto POSTDOC_DICYT COD 022043CR_POSTDOC
Informations de copyright
© 2022. The Author(s).
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