A LAMP sequencing approach for high-throughput co-detection of SARS-CoV-2 and influenza virus in human saliva.
COVID
RT-LAMP
human
infectious disease
microbiology
sequencing
testing
viruses
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
09 05 2022
09 05 2022
Historique:
received:
05
05
2021
accepted:
24
04
2022
entrez:
9
5
2022
pubmed:
10
5
2022
medline:
11
5
2022
Statut:
epublish
Résumé
The COVID-19 pandemic has created an urgent need for rapid, effective, and low-cost SARS-CoV-2 diagnostic testing. Here, we describe COV-ID, an approach that combines RT-LAMP with deep sequencing to detect SARS-CoV-2 in unprocessed human saliva with a low limit of detection (5-10 virions). Based on a multi-dimensional barcoding strategy, COV-ID can be used to test thousands of samples overnight in a single sequencing run with limited labor and laboratory equipment. The sequencing-based readout allows COV-ID to detect multiple amplicons simultaneously, including key controls such as host transcripts and artificial spike-ins, as well as multiple pathogens. Here, we demonstrate this flexibility by simultaneous detection of 4 amplicons in contrived saliva samples: SARS-CoV-2, influenza A, human
Identifiants
pubmed: 35532013
doi: 10.7554/eLife.69949
pii: 69949
pmc: PMC9084890
doi:
pii:
Substances chimiques
RNA, Viral
0
Banques de données
GEO
['GSE172118']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHLBI NIH HHS
ID : DP2 HL147123
Pays : United States
Informations de copyright
© 2022, Warneford-Thomson et al.
Déclaration de conflit d'intérêts
RW, PS, PL, JL, JM, AD, KZ, JS, RJ, CT No competing interests declared, BA Research funding from Becton Dickinson Speaking honoraria from Becton Dickinson, Stryker Equity in VOC Health, a company developing novel COVID19 detection technology distinct from the topic of this manuscript, RB Reviewing editor, eLife
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