Dogs as carriers of virulent and resistant genotypes of Clostridioides difficile.
Animals
Anti-Bacterial Agents
/ pharmacology
Anti-Infective Agents
/ pharmacology
Clindamycin
/ pharmacology
Clostridioides
Clostridioides difficile
/ genetics
Clostridium Infections
/ epidemiology
Dog Diseases
/ drug therapy
Dogs
Drug Resistance, Bacterial
/ genetics
Erythromycin
Genotype
Humans
Metronidazole
/ pharmacology
Microbial Sensitivity Tests
/ veterinary
Multilocus Sequence Typing
/ veterinary
Tetracyclines
Vancomycin
/ pharmacology
One Health
antimicrobial resistance
multilocus sequence typing
whole-genome sequencing
Journal
Zoonoses and public health
ISSN: 1863-2378
Titre abrégé: Zoonoses Public Health
Pays: Germany
ID NLM: 101300786
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
19
04
2022
received:
17
12
2021
accepted:
25
04
2022
pubmed:
13
5
2022
medline:
9
9
2022
entrez:
12
5
2022
Statut:
ppublish
Résumé
While previous research on zoonotic transmission of community-acquired Clostridioides difficile infection (CA-CDI) focused on food-producing animals, the present study aimed to investigate whether dogs are carriers of resistant and/or virulent C. difficile strains. Rectal swabs were collected from 323 dogs and 38 C. difficile isolates (11.8%) were obtained. Isolates were characterized by antimicrobial susceptibility testing, whole-genome sequencing (WGS) and a DNA hybridization assay. Multilocus sequence typing (MLST), core genome MLST (cgMLST) and screening for virulence and antimicrobial resistance genes were performed based on WGS. Minimum inhibitory concentrations for erythromycin, clindamycin, tetracycline, vancomycin and metronidazole were determined by E-test. Out of 38 C. difficile isolates, 28 (73.7%) carried genes for toxins. The majority of isolates belonged to MLST sequence types (STs) of clade I and one to clade V. Several isolates belonged to STs previously associated with human CA-CDI. However, cgMLST showed low genetic relatedness between the isolates of this study and C. difficile strains isolated from humans in Austria for which genome sequences were publicly available. Four isolates (10.5%) displayed resistance to three of the tested antimicrobial agents. Isolates exhibited resistance to erythromycin, clindamycin, tetracycline and metronidazole. These phenotypic resistances were supported by the presence of the resistance genes erm(B), cfr(C) and tet(M). All isolates were susceptible to vancomycin. Our results indicate that dogs may carry virulent and antimicrobial-resistant C. difficile strains.
Identifiants
pubmed: 35546073
doi: 10.1111/zph.12956
pmc: PMC9544694
doi:
Substances chimiques
Anti-Bacterial Agents
0
Anti-Infective Agents
0
Tetracyclines
0
Metronidazole
140QMO216E
Clindamycin
3U02EL437C
Erythromycin
63937KV33D
Vancomycin
6Q205EH1VU
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
673-681Informations de copyright
© 2022 The Authors. Zoonoses and Public Health published by Wiley-VCH GmbH.
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