Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from CO
Acetogens
Biofuels
Butanol
Hexanol
Syngas fermentation
Wood-Ljungdahl pathway
Journal
Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812
Informations de publication
Date de publication:
14 May 2022
14 May 2022
Historique:
received:
21
02
2022
accepted:
11
04
2022
entrez:
15
5
2022
pubmed:
16
5
2022
medline:
18
5
2022
Statut:
epublish
Résumé
The replacement of fossil fuels and petrochemicals with sustainable alternatives is necessary to mitigate the effects of climate change and also to counteract diminishing fossil resources. Acetogenic microorganisms such as Clostridium spp. are promising sources of fuels and basic chemical precursors because they efficiently utilize CO and CO Using a metabolic engineering approach we transferred a 17.9-kb gene cluster via conjugation, containing 13 genes from C. kluyveri and C. acetobutylicum for butanol and hexanol biosynthesis, into C. ljungdahlii. Plasmid-based expression resulted in 1075 mg L We thus confirmed the function of the butanol/hexanol biosynthesis genes and achieved hexanol biosynthesis in the syngas-fermenting species C. ljungdahlii for the first time, reaching the levels produced naturally by C. carboxidivorans. The genomic integration strain produced hexanol without selection and is therefore suitable for continuous fermentation processes.
Sections du résumé
BACKGROUND
BACKGROUND
The replacement of fossil fuels and petrochemicals with sustainable alternatives is necessary to mitigate the effects of climate change and also to counteract diminishing fossil resources. Acetogenic microorganisms such as Clostridium spp. are promising sources of fuels and basic chemical precursors because they efficiently utilize CO and CO
RESULTS
RESULTS
Using a metabolic engineering approach we transferred a 17.9-kb gene cluster via conjugation, containing 13 genes from C. kluyveri and C. acetobutylicum for butanol and hexanol biosynthesis, into C. ljungdahlii. Plasmid-based expression resulted in 1075 mg L
CONCLUSIONS
CONCLUSIONS
We thus confirmed the function of the butanol/hexanol biosynthesis genes and achieved hexanol biosynthesis in the syngas-fermenting species C. ljungdahlii for the first time, reaching the levels produced naturally by C. carboxidivorans. The genomic integration strain produced hexanol without selection and is therefore suitable for continuous fermentation processes.
Identifiants
pubmed: 35568911
doi: 10.1186/s12934-022-01802-8
pii: 10.1186/s12934-022-01802-8
pmc: PMC9107641
doi:
Substances chimiques
Butanols
0
Hexanols
0
Carbon Dioxide
142M471B3J
1-Butanol
8PJ61P6TS3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
85Subventions
Organisme : Horizon 2020 Framework Programme
ID : 761042
Organisme : Horizon 2020 Framework Programme
ID : 761042
Organisme : Bundesministerium für Bildung und Forschung
ID : 0315518
Informations de copyright
© 2022. The Author(s).
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