MNPmApp: An image analysis tool to quantify mononuclear phagocyte distribution in mucosal tissues.
Helicobacter pylori
Monte Carlo method
cell count
dendritic cells
epithelium
fluorescence
gastric mucosa
image analysis
macrophages
microscopy
software
tissue cytometry
Journal
Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930
Titre abrégé: Cytometry A
Pays: United States
ID NLM: 101235694
Informations de publication
Date de publication:
12 2022
12 2022
Historique:
revised:
27
03
2022
received:
22
06
2021
accepted:
12
05
2022
pubmed:
16
5
2022
medline:
15
12
2022
entrez:
15
5
2022
Statut:
ppublish
Résumé
Mononuclear phagocytes (MNPs) such as dendritic cells and macrophages perform key sentinel functions in mucosal tissues and are responsible for inducing and maintaining adaptive immune responses to mucosal pathogens. Positioning of MNPs at the epithelial interface facilitates their access to luminally-derived antigens and regulates MNP function through soluble mediators or surface receptor interactions. Therefore, accurately quantifying the distribution of MNPs within mucosal tissues as well as their spatial relationship with other cells is important to infer functional cellular interactions in health and disease. In this study, we developed and validated a MATLAB-based tissue cytometry platform, termed "MNP mapping application" (MNPmApp), that performs high throughput analyses of MNP density and distribution in the gastrointestinal mucosa based on digital multicolor fluorescence microscopy images and that integrates a Monte Carlo modeling feature to assess randomness of MNP distribution. MNPmApp identified MNPs in tissue sections of the human gastric mucosa with 98 ± 2% specificity and 76 ± 15% sensitivity for HLA-DR
Identifiants
pubmed: 35569131
doi: 10.1002/cyto.a.24657
pmc: PMC9663762
mid: NIHMS1808019
doi:
Substances chimiques
HLA-DR Antigens
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1012-1026Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM110732
Pays : United States
Organisme : NIBIB NIH HHS
ID : U01 EB029242
Pays : United States
Organisme : NIH HHS
ID : P20GM103474
Pays : United States
Organisme : NIH HHS
ID : P30GM110732
Pays : United States
Organisme : NIGMS NIH HHS
ID : P20 GM103474
Pays : United States
Informations de copyright
© 2022 International Society for Advancement of Cytometry.
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