Bioproduction of eriodictyol by Escherichia coli engineered co-culture.
Biosynthesis
Co-culture E. coli
Eriodictyol
Metabolic engineering
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
16 May 2022
16 May 2022
Historique:
received:
01
12
2021
accepted:
22
04
2022
entrez:
15
5
2022
pubmed:
16
5
2022
medline:
18
5
2022
Statut:
epublish
Résumé
Eriodictyol (ED) is a flavonoid in the flavanones subclass. It is abundantly present in a wide range of medicinal plants, citrus fruits, and vegetables. In addition, ED owns numerous importantly medicinal bioactivities such as inhibition of proliferation, metastasis and induction of apoptosis in glioma cells or inhibition of glioblastoma migration, and invasion. This study described the heterologous production of ED by E. coli based co-culture engineering system from the simple carbon substrate D-glucose. Two E. coli strains were engineered and functioned as constitutive components of biological system. Specifically, the first strain (upstream module) contained genes for synthesis of p-coumaric acid (pCA) from D-glucose. And, the second strain (downstream module) consisted of genes for the synthesis of ED from pCA. The highest yield in ED production was achieved 51.5 ± 0.4 mg/L using stepwise optimal culture conditions, while monoculture was achieved 21.3 ± 0.2 mg/L only. In conclusion, co-culture was the most efficient alternative approach for the synthesis of ED and other natural products.
Identifiants
pubmed: 35570219
doi: 10.1007/s11274-022-03294-5
pii: 10.1007/s11274-022-03294-5
doi:
Substances chimiques
Flavanones
0
Glucose
IY9XDZ35W2
eriodictyol
Q520486B8Y
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112Subventions
Organisme : National Foundation for Science and Technology Development
ID : 106.02-2018.326
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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