HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 05 2022
18 05 2022
Historique:
received:
22
04
2021
accepted:
13
04
2022
entrez:
18
5
2022
pubmed:
19
5
2022
medline:
21
5
2022
Statut:
epublish
Résumé
The biological role of RNA-binding proteins in the secretory pathway is not well established. Here, we describe that human HDLBP/Vigilin directly interacts with more than 80% of ER-localized mRNAs. PAR-CLIP analysis reveals that these transcripts represent high affinity HDLBP substrates and are specifically bound in their coding sequences (CDS), in contrast to CDS/3'UTR-bound cytosolic mRNAs. HDLBP crosslinks strongly to long CU-rich motifs, which frequently reside in CDS of ER-localized mRNAs and result in high affinity multivalent interactions. In addition to HDLBP-ncRNA interactome, quantification of HDLBP-proximal proteome confirms association with components of the translational apparatus and the signal recognition particle. Absence of HDLBP results in decreased translation efficiency of HDLBP target mRNAs, impaired protein synthesis and secretion in model cell lines, as well as decreased tumor growth in a lung cancer mouse model. These results highlight a general function for HDLBP in the translation of ER-localized mRNAs and its relevance for tumor progression.
Identifiants
pubmed: 35585045
doi: 10.1038/s41467-022-30322-7
pii: 10.1038/s41467-022-30322-7
pmc: PMC9117268
doi:
Substances chimiques
3' Untranslated Regions
0
Membrane Proteins
0
RNA, Messenger
0
RNA-Binding Proteins
0
Signal Recognition Particle
0
high density lipoprotein binding protein
147605-06-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2727Informations de copyright
© 2022. The Author(s).
Références
Mol Cell. 2018 Feb 1;69(3):517-532.e11
pubmed: 29395067
Nucleic Acids Res. 2007;35(6):e43
pubmed: 17311810
Anal Chem. 2003 Feb 1;75(3):663-70
pubmed: 12585499
Cell Rep. 2018 Jul 3;24(1):20-26
pubmed: 29972781
Methods. 2017 Apr 15;118-119:24-40
pubmed: 27765618
Nat Genet. 2014 Sep;46(9):964-72
pubmed: 25129143
Curr Opin Chem Biol. 2019 Feb;48:44-54
pubmed: 30458335
Nature. 2016 Nov 30;540(7631):134-138
pubmed: 27905431
Mol Biol Cell. 2011 Jul 15;22(14):2646-58
pubmed: 21613539
Nucleic Acids Res. 2018 Jul 6;46(12):6285-6303
pubmed: 29660014
PLoS Genet. 2019 Jul 1;15(7):e1008248
pubmed: 31260446
Mol Cell. 2011 Aug 5;43(3):340-52
pubmed: 21723171
Nat Microbiol. 2019 Dec;4(12):2369-2382
pubmed: 31384002
RNA. 2008 Mar;14(3):445-53
pubmed: 18192611
Annu Rev Biophys. 2014;43:381-408
pubmed: 24895856
Methods. 2008 Jan;44(1):3-12
pubmed: 18158127
EMBO Rep. 2004 Dec;5(12):1137-41
pubmed: 15577927
Methods Mol Biol. 2011;714:301-21
pubmed: 21431749
Mol Cell. 2014 May 22;54(4):573-85
pubmed: 24726324
Nat Commun. 2016 Sep 26;7:12848
pubmed: 27665711
Genes Dev. 2020 Aug 1;34(15-16):1075-1088
pubmed: 32616520
Eur J Biochem. 1993 Apr 15;213(2):727-36
pubmed: 8477745
Oncol Rep. 2014 May;31(5):2328-34
pubmed: 24676454
PLoS Biol. 2007 Dec;5(12):e322
pubmed: 18052610
Nature. 2016 Aug 11;536(7615):224-8
pubmed: 27487213
Genome Biol. 2009;10(3):R25
pubmed: 19261174
Curr Protoc Mol Biol. 2019 Dec;129(1):e108
pubmed: 31763789
Nat Struct Mol Biol. 2014 Dec;21(12):1100-5
pubmed: 25420103
Genome Biol. 2014 Jan 13;15(1):R15
pubmed: 24417896
Nat Methods. 2016 Jun;13(6):508-14
pubmed: 27018577
J Biol Chem. 2013 Oct 11;288(41):29633-41
pubmed: 24019514
Nat Struct Mol Biol. 2019 Jul;26(7):637-648
pubmed: 31270472
Cell. 2017 Jun 1;169(6):1051-1065.e18
pubmed: 28575669
Nucleic Acids Res. 2014 Apr;42(6):4043-55
pubmed: 24445806
Proc Natl Acad Sci U S A. 2017 Dec 26;114(52):13691-13696
pubmed: 29229848
Proc Natl Acad Sci U S A. 2020 Mar 17;117(11):5883-5894
pubmed: 32132204
Cell Rep. 2017 Aug 8;20(6):1463-1475
pubmed: 28793268
Chembiochem. 2000 Aug 18;1(2):86-102
pubmed: 11828402
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Mol Biol. 2004 Jul 16;340(4):783-95
pubmed: 15223320
Curr Opin Struct Biol. 2015 Feb;30:63-70
pubmed: 25625331
J Cell Biol. 1964 Mar;20:473-95
pubmed: 14128049
Cell. 1996 Apr 19;85(2):237-45
pubmed: 8612276
Nature. 2007 Nov 29;450(7170):663-9
pubmed: 18046402
Mol Cell Biol. 2011 Jan;31(1):215-25
pubmed: 20974809
Methods Mol Biol. 2012;941:181-93
pubmed: 23065562
Biochim Biophys Acta. 2011 Jan;1809(1):46-55
pubmed: 21035573
Science. 2009 Apr 10;324(5924):218-23
pubmed: 19213877
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Proteomics. 2009 Jan;9(1):205-9
pubmed: 19053139
Science. 2018 Apr 20;360(6386):323-327
pubmed: 29567807
Nucleic Acids Res. 2018 Jan 4;46(D1):D303-D307
pubmed: 29106616
Methods Mol Biol. 2016;1358:197-207
pubmed: 26463385
Biochemistry. 1990 May 29;29(21):4997-5008
pubmed: 2198935
Nature. 2013 Jul 11;499(7457):172-7
pubmed: 23846655
J Proteome Res. 2011 Apr 1;10(4):1794-805
pubmed: 21254760
Nature. 2012 Dec 20;492(7429):382-6
pubmed: 23235829
Mol Cell Biol. 2009 Mar;29(6):1626-34
pubmed: 19114558
J Biol Chem. 2001 May 11;276(19):15905-12
pubmed: 11278502
J Cell Biol. 1981 Nov;91(2 Pt 1):551-6
pubmed: 7309796
Mol Cell. 2015 Oct 15;60(2):208-19
pubmed: 26412307
Curr Biol. 2020 Mar 9;30(5):854-864.e5
pubmed: 31956032
Anal Biochem. 2006 Mar 15;350(2):222-32
pubmed: 16448619
Nucleic Acids Res. 2019 May 7;47(8):4334-4348
pubmed: 30864660
Nat Commun. 2013;4:2784
pubmed: 24253588
RNA. 2003 Sep;9(9):1123-37
pubmed: 12923260
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
J Cell Biol. 1975 Dec;67(3):835-51
pubmed: 811671
Nat Rev Mol Cell Biol. 2015 Apr;16(4):221-31
pubmed: 25735911
J Biol Chem. 2000 Oct 27;275(43):33820-7
pubmed: 10931837
Nucleic Acids Res. 2016 Jan 4;44(D1):D1251-7
pubmed: 26450961
Nucleic Acids Res. 2018 Mar 16;46(5):2678-2689
pubmed: 29401259
J Mol Biol. 2001 Jan 19;305(3):567-80
pubmed: 11152613
Mol Biol Cell. 2013 Oct;24(19):3069-84
pubmed: 23904265
Nature. 2013 May 2;497(7447):80-5
pubmed: 23636399
J Biol Chem. 2012 Feb 17;287(8):5518-27
pubmed: 22199352
Nat Rev Mol Cell Biol. 2018 Aug;19(8):526-541
pubmed: 29760421
PLoS Comput Biol. 2018 Aug 13;14(8):e1006169
pubmed: 30102689
Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6662-6
pubmed: 19366666
Genome Res. 2017 Aug;27(8):1344-1359
pubmed: 28596291
Genes Dev. 2009 Jul 1;23(13):1559-70
pubmed: 19571182
Mol Cell. 2018 Jun 7;70(5):854-867.e9
pubmed: 29883606
Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1531-6
pubmed: 21205896
Nat Struct Mol Biol. 2010 Jul;17(7):909-15
pubmed: 20601959
Sci Signal. 2013 Nov 19;6(302):rs15
pubmed: 24255178
Cell. 2020 Feb 6;180(3):568-584.e23
pubmed: 31981491
Cell Rep. 2017 Dec 26;21(13):3740-3753
pubmed: 29281824