Genomic biosurveillance detects a sexual hybrid in the sudden oak death pathogen.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
19 05 2022
19 05 2022
Historique:
received:
13
10
2021
accepted:
21
04
2022
entrez:
19
5
2022
pubmed:
20
5
2022
medline:
24
5
2022
Statut:
epublish
Résumé
Invasive exotic pathogens pose a threat to trees and forest ecosystems worldwide, hampering the provision of essential ecosystem services such as carbon sequestration and water purification. Hybridization is a major evolutionary force that can drive the emergence of pathogens. Phytophthora ramorum, an emergent pathogen that causes the sudden oak and larch death, spreads as reproductively isolated divergent clonal lineages. We use a genomic biosurveillance approach by sequencing genomes of P. ramorum from survey and inspection samples and report the discovery of variants of P. ramorum that are the result of hybridization via sexual recombination between North American and European lineages. We show that these hybrids are viable, can infect a host and produce spores for long-term survival and propagation. Genome sequencing revealed genotypic combinations at 54,515 single nucleotide polymorphism loci not present in parental lineages. More than 6,000 of those genotypes are predicted to have a functional impact in genes associated with host infection, including effectors, carbohydrate-active enzymes and proteases. We also observed post-meiotic mitotic recombination that could generate additional genotypic and phenotypic variation and contribute to homoploid hybrid speciation. Our study highlights the importance of plant pathogen biosurveillance to detect variants, including hybrids, and inform management and control.
Identifiants
pubmed: 35589982
doi: 10.1038/s42003-022-03394-w
pii: 10.1038/s42003-022-03394-w
pmc: PMC9120034
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
477Informations de copyright
© 2022. The Author(s).
Références
PLoS Pathog. 2021 Nov 10;17(11):e1009957
pubmed: 34758051
Bioinformatics. 2012 Dec 15;28(24):3326-8
pubmed: 23060615
PLoS Pathog. 2012 Sep;8(9):e1002893
pubmed: 23028308
J Comput Biol. 2015 Jun;22(6):498-509
pubmed: 25658651
Nat Genet. 2016 Feb;48(2):201-5
pubmed: 26752267
Plant Dis. 2018 Aug 8;:PDIS02180288PDN
pubmed: 30088960
Fly (Austin). 2012 Apr-Jun;6(2):80-92
pubmed: 22728672
Mol Ecol. 2001 Mar;10(3):551-68
pubmed: 11298968
Mol Plant Microbe Interact. 2022 Apr;35(4):360-363
pubmed: 35285670
New Phytol. 2009;183(4):993-1000
pubmed: 19558422
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
J Fungi (Basel). 2021 Mar 18;7(3):
pubmed: 33803849
Phytopathology. 2015 Jul;105(7):966-81
pubmed: 25760519
Annu Rev Phytopathol. 2019 Aug 25;57:301-321
pubmed: 31226018
Yeast. 2018 Jan;35(1):5-20
pubmed: 28681409
PLoS Biol. 2019 Oct 15;17(10):e3000490
pubmed: 31613878
Curr Genet. 2007 May;51(5):285-96
pubmed: 17310332
Fungal Biol. 2010 Apr;114(4):369-78
pubmed: 20943147
Nature. 2000 May 11;405(6783):134-5
pubmed: 10821256
Nature. 2012 Apr 11;484(7393):186-94
pubmed: 22498624
Genom Data. 2017 Feb 06;12:17-21
pubmed: 28243575
mBio. 2019 Mar 12;10(2):
pubmed: 30862749
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Bioinformatics. 2016 Jan 15;32(2):292-4
pubmed: 26428292
Bioinformatics. 2014 May 1;30(9):1312-3
pubmed: 24451623
Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8791-6
pubmed: 24889615
Mol Biol Evol. 2006 Feb;23(2):254-67
pubmed: 16221896
Elife. 2015 Feb 27;4:
pubmed: 25723966
Phytopathology. 2007 May;97(5):632-42
pubmed: 18943583
Syst Biol. 2018 Sep 1;67(5):821-829
pubmed: 29562307
Nucleic Acids Res. 1992 Nov 25;20(22):6115-6
pubmed: 1461751
Nat Rev Microbiol. 2013 Nov;11(11):800-14
pubmed: 24129511
Phytopathology. 2016 Feb;106(2):104-12
pubmed: 26824768
Am J Bot. 2014 Aug;101(8):1247-58
pubmed: 25156978
PLoS Pathog. 2015 Sep 03;11(9):e1005098
pubmed: 26336070
Science. 2013 Nov 15;342(6160):1235773
pubmed: 24233727
mBio. 2011 Jul 26;2(4):
pubmed: 21791579
Plant Dis. 2000 Jul;84(7):731-735
pubmed: 30832099
Mol Ecol. 2009 Mar;18(6):1161-74
pubmed: 19222751
Mycol Res. 2004 Jul;108(Pt 7):823-7
pubmed: 15446716
Phytopathology. 2009 Jul;99(7):792-5
pubmed: 19522576
Evol Appl. 2019 Sep 10;13(1):95-115
pubmed: 31892946
Nature. 2010 Aug 12;466(7308):824-5
pubmed: 20703294
PLoS Genet. 2007 Apr 27;3(4):e68
pubmed: 17465683
Nat Ecol Evol. 2020 Apr;4(4):626-638
pubmed: 32123324
Mol Ecol Resour. 2013 Sep;13(5):946-52
pubmed: 23738873
PLoS Genet. 2015 Oct 30;11(10):e1005626
pubmed: 26517373
Fungal Genet Biol. 2011 May;48(5):537-43
pubmed: 21272658
Heredity (Edinb). 1999 Oct;83 ( Pt 4):363-72
pubmed: 10583537
Annu Rev Phytopathol. 2006;44:41-60
pubmed: 16448329