Effect of osmotic stress on live cell plasma membranes, probed via Laurdan general polarization measurements.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
21 06 2022
21 06 2022
Historique:
received:
08
06
2021
revised:
10
09
2021
accepted:
16
05
2022
pubmed:
22
5
2022
medline:
25
6
2022
entrez:
21
5
2022
Statut:
ppublish
Résumé
Here we seek to gain insight into changes in the plasma membrane of live cells upon the application of osmotic stress using Laurdan, a fluorescent probe that reports on membrane organization, hydration, and dynamics. It is known that the application of osmotic stress to lipid vesicles causes a decrease in Laurdan's generalized polarization (GP), which has been interpreted as an indication of membrane stretching. In cells, we see the opposite effects, as GP increases when the osmolarity of the solution is decreased. This increase in GP is associated with the presence of caveolae, which are known to disassemble and flatten in response to osmotic stress.
Identifiants
pubmed: 35596525
pii: S0006-3495(22)00409-X
doi: 10.1016/j.bpj.2022.05.016
pmc: PMC9279170
pii:
doi:
Substances chimiques
Fluorescent Dyes
0
Laurates
0
2-Naphthylamine
CKR7XL41N4
laurdan
Y97FBL93VW
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
2411-2418Informations de copyright
Copyright © 2022 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11411-6
pubmed: 21709267
Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22050-4
pubmed: 21131568
Biophys J. 2013 Mar 19;104(6):1238-47
pubmed: 23528083
Biochim Biophys Acta. 2005 Dec 30;1746(3):260-73
pubmed: 15951036
Circulation. 2003 Aug 5;108(5):616-22
pubmed: 12835226
Langmuir. 2008 Jan 1;24(1):288-95
pubmed: 18044937
Biochemistry. 2001 May 22;40(20):6016-26
pubmed: 11352737
Biophys J. 2018 Oct 2;115(7):1307-1315
pubmed: 30219285
Integr Biol (Camb). 2016 Feb;8(2):216-29
pubmed: 26787445
Biophys J. 2018 Oct 16;115(8):1498-1508
pubmed: 30269886
Nat Rev Mol Cell Biol. 2007 Mar;8(3):185-94
pubmed: 17318224
Biophys J. 1998 Jul;75(1):331-41
pubmed: 9649390
J Cell Sci. 2013 Mar 1;126(Pt 5):1227-34
pubmed: 23378020
Annu Rev Biochem. 1998;67:199-225
pubmed: 9759488
Anal Chem. 2012 Oct 16;84(20):8650-5
pubmed: 22985263
J Biol Chem. 2015 Nov 6;290(45):27271-27279
pubmed: 26363067
Methods Enzymol. 2003;360:481-500
pubmed: 12622164
Nat Methods. 2010 Mar;7(3):170-1
pubmed: 20195248
FEBS J. 2013 Jun;280(12):2775-84
pubmed: 23438106
J Biol Chem. 2015 Jul 3;290(27):16698-707
pubmed: 25957403
Biophys J. 1994 Mar;66(3 Pt 1):763-8
pubmed: 8011908
Am J Physiol Heart Circ Physiol. 2000 Apr;278(4):H1285-93
pubmed: 10749726
J Fluoresc. 1995 Mar;5(1):59-69
pubmed: 24226612
Am J Physiol Heart Circ Physiol. 2003 Oct;285(4):H1720-9
pubmed: 12816751
Free Radic Biol Med. 2018 Nov 20;128:144-156
pubmed: 29885356
Cell. 2011 Feb 4;144(3):402-13
pubmed: 21295700
ACS Chem Biol. 2008 May 16;3(5):287-93
pubmed: 18484709
Cell. 2008 Jan 11;132(1):113-24
pubmed: 18191225
Exp Cell Res. 1992 Oct;202(2):432-9
pubmed: 1397095
Traffic. 2010 Mar;11(3):361-82
pubmed: 20070607
J Phys D Appl Phys. 2017 Apr 5;50(13):134004
pubmed: 29449744
Sci Rep. 2020 May 21;10(1):8395
pubmed: 32439841
J Biol Chem. 2005 Jul 22;280(29):26796-804
pubmed: 15917253
Mol Neurobiol. 2020 Feb;57(2):910-925
pubmed: 31595461
J Cell Sci. 1979 Feb;35:229-43
pubmed: 370129
Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3165-70
pubmed: 17360623
Methods Mol Biol. 2015;1232:273-90
pubmed: 25331141
Science. 1972 Feb 18;175(4023):720-31
pubmed: 4333397
Biochem J. 2015 Oct 1;471(1):101-9
pubmed: 26232493
Biochim Biophys Acta. 2016 Nov;1858(11):2625-2635
pubmed: 27480804
J Biol Chem. 2008 Feb 15;283(7):4314-22
pubmed: 18056712
J Lipid Res. 2009 Aug;50(8):1609-20
pubmed: 19074371
Biophys J. 1996 Aug;71(2):885-91
pubmed: 8842227
Biochem Soc Trans. 2020 Feb 28;48(1):147-154
pubmed: 31922193
J Biol Chem. 2012 Jan 20;287(4):2678-88
pubmed: 22134919
Biophys J. 1997 Jun;72(6):2413-29
pubmed: 9168019
Acc Chem Res. 2021 Feb 16;54(4):976-987
pubmed: 33513300
Biophys J. 1994 Jan;66(1):120-32
pubmed: 8130331
J Biol Chem. 2017 Mar 3;292(9):3779-3788
pubmed: 28096469
Am J Physiol Heart Circ Physiol. 2003 Sep;285(3):H1113-22
pubmed: 12763750
Photochem Photobiol. 1993 Mar;57(3):403-10
pubmed: 8475171
Biochimie. 2012 Jan;94(1):26-32
pubmed: 21740953
J Chem Phys. 2014 Dec 14;141(22):22D516
pubmed: 25494787
Int J Mol Sci. 2013 Dec 27;15(1):261-76
pubmed: 24378851
Cell Tissue Res. 2014 Sep;357(3):505-13
pubmed: 25107607
Platelets. 1994;5(3):145-8
pubmed: 21043628
J Mol Biol. 2017 Jul 7;429(14):2231-2245
pubmed: 28549925
Science. 2001 Nov 30;294(5548):1862-5
pubmed: 11729300
Cell Biochem Biophys. 2014 Nov;70(2):785-94
pubmed: 24839062
Biophys J. 1991 Jul;60(1):179-89
pubmed: 1883937