A new ferritin SjFer0 affecting the growth and development of Schistosoma japonicum.

Animals Annexins / genetics Female Ferritins / genetics Growth and Development Iron / metabolism Phylogeny RNA / metabolism Schistosoma japonicum / physiology Schistosomiasis japonica / parasitology
Ferritin Growth and development RNA interference Schistosoma japonicum

Journal

Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774

Informations de publication

Date de publication:
24 May 2022
Historique:
received: 08 09 2021
accepted: 21 03 2022
entrez: 24 5 2022
pubmed: 25 5 2022
medline: 27 5 2022
Statut: epublish

Résumé

Schistosomiasis, an acute and chronic parasitic disease, causes substantial morbidity and mortality in tropical and subtropical regions of the world. Iron is an essential constituent of numerous macromolecules involving in important cellular reactions in virtually all organisms. Trematodes of the genus Schistosoma live in iron-rich blood, feed on red blood cells and store abundant iron in vitelline cells. Ferritins are multi-meric proteins that store iron inside cells. Three ferritin isoforms in Schistosoma japonicum are known, namely SjFer0, SjFer1 and SjFer2; however, their impact on the growth and development of the parasites  is still unknown. In this study we report on and characterize the ferritins in S. japonicum. A phylogenetic tree of the SjFer0, SjFer1 and SjFer2 genes was constructed to show the evolutionary relationship among species of genus Schistosoma. RNA interference in vivo was used to investigate the impact of SjFer0 on schistosome growth and development.  Immunofluorescence assay was applied  to localize the expression of the ferritins. RNA-sequencing was performed  to characterize the iron transport profile after RNA interference. SjFer0 was found to have low similarity with SjFer1 and SjFer2 and contain an additional signal peptide sequence. Phylogenetic analysis revealed that SjFer0 can only cluster with some ferritins of other trematodes and tapeworms, suggesting that this ferritin branch might be unique to these parasites. RNA interference in vivo showed that SjFer0 significantly affected the growth and development of schistosomula but did not affect egg production of adult female worms. SjFer1 and SjFer2 had no significant impact on growth and development. The immunofluorescence study showed that SjFer0 was widely expressed in the somatic cells and vitelline glands but not in the testicle or ovary. RNA-sequencing indicated  that, in female, the ion transport process and calcium ion binding function were downregulated after SjFer0 RNA interference. Among the differentially downregulated genes, Sj-cpi-2, annexin and insulin-like growth factor-binding protein may be accounted for the suppression of schistosome growth and development. The results indicate that SjFer0 affects the growth and development of schistosomula but does not affect egg production of adult female worms. SjFer0 can rescue the growth of the fet3fet4 double mutant Saccharomyces cerevisiae (strain DEY1453), suggesting being able to promote iron absorption. The RNA interference of SjFer0 inferred that  the suppression of worm growth and development may via  down-regulating Sj-cpi-2, annexin, and IGFBP.

Sections du résumé

BACKGROUND BACKGROUND
Schistosomiasis, an acute and chronic parasitic disease, causes substantial morbidity and mortality in tropical and subtropical regions of the world. Iron is an essential constituent of numerous macromolecules involving in important cellular reactions in virtually all organisms. Trematodes of the genus Schistosoma live in iron-rich blood, feed on red blood cells and store abundant iron in vitelline cells. Ferritins are multi-meric proteins that store iron inside cells. Three ferritin isoforms in Schistosoma japonicum are known, namely SjFer0, SjFer1 and SjFer2; however, their impact on the growth and development of the parasites  is still unknown. In this study we report on and characterize the ferritins in S. japonicum.
METHODS METHODS
A phylogenetic tree of the SjFer0, SjFer1 and SjFer2 genes was constructed to show the evolutionary relationship among species of genus Schistosoma. RNA interference in vivo was used to investigate the impact of SjFer0 on schistosome growth and development.  Immunofluorescence assay was applied  to localize the expression of the ferritins. RNA-sequencing was performed  to characterize the iron transport profile after RNA interference.
RESULTS RESULTS
SjFer0 was found to have low similarity with SjFer1 and SjFer2 and contain an additional signal peptide sequence. Phylogenetic analysis revealed that SjFer0 can only cluster with some ferritins of other trematodes and tapeworms, suggesting that this ferritin branch might be unique to these parasites. RNA interference in vivo showed that SjFer0 significantly affected the growth and development of schistosomula but did not affect egg production of adult female worms. SjFer1 and SjFer2 had no significant impact on growth and development. The immunofluorescence study showed that SjFer0 was widely expressed in the somatic cells and vitelline glands but not in the testicle or ovary. RNA-sequencing indicated  that, in female, the ion transport process and calcium ion binding function were downregulated after SjFer0 RNA interference. Among the differentially downregulated genes, Sj-cpi-2, annexin and insulin-like growth factor-binding protein may be accounted for the suppression of schistosome growth and development.
CONCLUSIONS CONCLUSIONS
The results indicate that SjFer0 affects the growth and development of schistosomula but does not affect egg production of adult female worms. SjFer0 can rescue the growth of the fet3fet4 double mutant Saccharomyces cerevisiae (strain DEY1453), suggesting being able to promote iron absorption. The RNA interference of SjFer0 inferred that  the suppression of worm growth and development may via  down-regulating Sj-cpi-2, annexin, and IGFBP.

Identifiants

DOI: 10.1186/s13071-022-05247-1 PMID: 35610663 PMC: PMC9128280
pubmed: 35610663
doi: 10.1186/s13071-022-05247-1
pii: 10.1186/s13071-022-05247-1
pmc: PMC9128280
doi:

Substances chimiques

Annexins 0
RNA 63231-63-0
Ferritins 9007-73-2
Iron E1UOL152H7

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

177

Subventions

Organisme : National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
ID : 31725025
Organisme : Program of Shanghai Academic Research Leader
ID : 18XD1400400

Informations de copyright

© 2022. The Author(s).

Références

Mol Biochem Parasitol. 2012 Mar-Apr;182(1-2):75-82
pubmed: 22245333
Nat Genet. 1997 Aug;16(4):383-6
pubmed: 9241278
Int J Parasitol Drugs Drug Resist. 2018 Aug;8(2):341-349
pubmed: 29957332
Int J Biochem Cell Biol. 2007;39(9):1646-58
pubmed: 17556009
PLoS One. 2014 Jun 04;9(6):e98792
pubmed: 24896847
Cell Metab. 2011 Nov 2;14(5):647-57
pubmed: 22055506
Bioinformatics. 2018 Sep 1;34(17):i884-i890
pubmed: 30423086
Nat Commun. 2017 Mar 13;8:14693
pubmed: 28287085
FEBS J. 2014 Feb;281(4):1209-25
pubmed: 24428567
J Exp Med. 2005 Oct 3;202(7):955-65
pubmed: 16203866
J Cell Sci. 2007 Jun 15;120(Pt 12):2066-77
pubmed: 17535853
Dev Cell. 2009 Jan;16(1):35-46
pubmed: 19154717
Mov Disord. 2004 Jan;19(1):36-42
pubmed: 14743358
Cell. 2012 May 25;149(5):1060-72
pubmed: 22632970
Physiol Rev. 2002 Apr;82(2):331-71
pubmed: 11917092
J Biol Chem. 2006 Sep 22;281(38):28415-29
pubmed: 16857685
J Biol Chem. 2008 Jan 11;283(2):716-25
pubmed: 18024960
J Cell Biol. 1996 Sep;134(5):1179-95
pubmed: 8794860
Pathogens. 2020 May 30;9(6):
pubmed: 32486220
Biochem Biophys Res Commun. 2018 Sep 5;503(2):1004-1010
pubmed: 29935182
Proc Natl Acad Sci U S A. 1996 May 28;93(11):5624-8
pubmed: 8643627
Mol Reprod Dev. 1995 Jul;41(3):325-30
pubmed: 8588931
Mol Biol Cell. 1999 Jul;10(7):2329-42
pubmed: 10397768
Mol Biochem Parasitol. 1992 Feb;50(2):245-54
pubmed: 1741011
FEBS J. 2015 Sep;282(18):3632-46
pubmed: 26153121
Nat Genet. 2005 Nov;37(11):1264-9
pubmed: 16227996
Genome Biol. 2014;15(12):550
pubmed: 25516281
Diabetes Res Clin Pract. 2007 May;76(2):177-86
pubmed: 17011663
Cell. 2017 Jan 26;168(3):344-361
pubmed: 28129536
Methods Mol Biol. 2020;2151:27-41
pubmed: 32451993
Nucleic Acids Res. 2018 Jan 4;46(D1):D493-D496
pubmed: 29040681
Cytoskeleton (Hoboken). 2013 Jun;70(6):304-16
pubmed: 23606669
Chem Biol. 2008 Mar;15(3):234-45
pubmed: 18355723
J Biol Chem. 1988 Jun 25;263(18):8938-42
pubmed: 3379054
Toxicol Lett. 1995 Dec;82-83:969-74
pubmed: 8597169
PLoS Genet. 2011 Dec;7(12):e1002394
pubmed: 22194696
Nature. 2006 Mar 2;440(7080):96-100
pubmed: 16511496
Eur J Biochem. 1996 Oct 1;241(1):64-9
pubmed: 8898889
J Parasitol. 1989 Jun;75(3):417-21
pubmed: 2786065
Parasitol Res. 2017 Mar;116(3):901-908
pubmed: 28066871
Chem Commun (Camb). 2014 Dec 18;50(97):15358-61
pubmed: 25348725
Cell Metab. 2015 Nov 3;22(5):777-87
pubmed: 26437604
Curr Biol. 2001 Mar 20;11(6):447-51
pubmed: 11301256
Proc Natl Acad Sci U S A. 2009 Sep 22;106(38):16263-8
pubmed: 19805291
BMC Bioinformatics. 2011 Aug 04;12:323
pubmed: 21816040
Cell Death Differ. 2016 Mar;23(3):369-79
pubmed: 26794443
Free Radic Biol Med. 2002 Aug 15;33(4):457-63
pubmed: 12160928
Nat Rev Dis Primers. 2018 Aug 9;4(1):13
pubmed: 30093684
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
EBioMedicine. 2015 Oct 04;2(11):1705-17
pubmed: 26870796
PLoS Negl Trop Dis. 2010 Nov 16;4(11):e884
pubmed: 21103361
Science. 2008 May 30;320(5880):1207-10
pubmed: 18511687
Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13612-7
pubmed: 16950869
Annu Rev Nutr. 1998;18:441-69
pubmed: 9706232
Nature. 1997 Jul 31;388(6641):482-8
pubmed: 9242408
Cell Rep. 2015 Oct 20;13(3):533-545
pubmed: 26456827
J Biol Chem. 2003 Jan 31;278(5):3227-34
pubmed: 12438312
J Biol Chem. 2006 Jan 27;281(4):2242-8
pubmed: 16267047
Nat Chem Biol. 2006 Aug;2(8):406-14
pubmed: 16850017

Auteurs

Fanyuan Zeng (F)

State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 2005 Song Hu Road, Shanghai, 200438, People's Republic of China.

Cun Yi (C)

State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 2005 Song Hu Road, Shanghai, 200438, People's Republic of China.

Wei Zhang (W)

State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 2005 Song Hu Road, Shanghai, 200438, People's Republic of China.

Shaoyun Cheng (S)

State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 2005 Song Hu Road, Shanghai, 200438, People's Republic of China.

Chengsong Sun (C)

State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 2005 Song Hu Road, Shanghai, 200438, People's Republic of China.

Fang Luo (F)

State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 2005 Song Hu Road, Shanghai, 200438, People's Republic of China.

Zheng Feng (Z)

Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, WHO Collaborating Center for Tropical Diseases, Joint Research Laboratory of Genetics and Ecology on Parasite-Host Interaction, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, 200025, People's Republic of China.

Wei Hu (W)

State Key Laboratory of Genetic Engineering, Ministry of Education Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, 2005 Song Hu Road, Shanghai, 200438, People's Republic of China. huw@fudan.edu.cn.
Key Laboratory of Parasite and Vector Biology of the Chinese Ministry of Health, WHO Collaborating Center for Tropical Diseases, Joint Research Laboratory of Genetics and Ecology on Parasite-Host Interaction, National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, 200025, People's Republic of China. huw@fudan.edu.cn.
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Monglia University, Hohhot, 010030, People's Republic of China. huw@fudan.edu.cn.

Articles similaires

Comprehensive comparative analysis and development of molecular markers for Lasianthus species based on complete chloroplast genome sequences.

Yue Zhang, Meifang Song, Deying Tang et al.
1.00
Genome, Chloroplast Phylogeny Genetic Markers Base Composition High-Throughput Nucleotide Sequencing

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A new ferritin SjFer0 affecting the growth and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Protéines de liaison au calcium › Annexines › A new ferritin SjFer0 affecting the growth and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Métalloprotéines › Protéines de liaison au fer › Ferritines › A new ferritin SjFer0 affecting the growth and...
questionsmedicales.fr › Phénomènes physiologiques › Croissance et développement › A new ferritin SjFer0 affecting the growth and...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Fer › A new ferritin SjFer0 affecting the growth and...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › A new ferritin SjFer0 affecting the growth and...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › A new ferritin SjFer0 affecting the growth and...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Helminthes › Plathelminthes › Trematoda › Schistosomatidae › Schistosoma › Schistosoma japonicum › A new ferritin SjFer0 affecting the growth and...
questionsmedicales.fr › Infection › Maladies vectorielles › Schistosomiase › Schistosomiase artérioveineuse › A new ferritin SjFer0 affecting the growth and...