LINC00174 promotes cell proliferation and metastasis in renal clear cell carcinoma by regulating miR-612/FOXM1 axis.
FOXM1
KIRC
LINC00174
miR-612
proliferation
Journal
Immunopharmacology and immunotoxicology
ISSN: 1532-2513
Titre abrégé: Immunopharmacol Immunotoxicol
Pays: England
ID NLM: 8800150
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
pubmed:
27
5
2022
medline:
5
10
2022
entrez:
26
5
2022
Statut:
ppublish
Résumé
Kidney renal clear cell carcinoma (KIRC) is the most common pathological subtype of kidney tumor. Reportedly, LINC00174 is a key regulator in cancer progression. This study aims to clarify the role and molecular mechanism of LINC00174 in the progression of KIRC. LINC00174 expression in KIRC and its prognostic value were analyzed by bioinformatics. LINC00174, miR-612 and FOXM1 mRNA expression levels in KIRC clinical samples and cell lines were detected by qRT-PCR. After LINC00174 was overexpressed or knocked down, CCK-8, BrdU and Transwell assays were adopted to evaluate the proliferation and metastatic potential of KIRC cells. Bioinformatics and dual luciferase reporter assays were employed to validate the targeting relationship between miR-612 and LINC00174 or FOXM1 mRNA, respectively. Western blot assay was performed to detect FOXM1 protein expression in KIRC cells. LINC00174 expression and FOXM1 expression were up-regulated in 42 cases of KIRC tissues ( LINC00174 facilitates the proliferation and metastatic potential of KIRC cells
Sections du résumé
BACKGROUND
UNASSIGNED
Kidney renal clear cell carcinoma (KIRC) is the most common pathological subtype of kidney tumor. Reportedly, LINC00174 is a key regulator in cancer progression. This study aims to clarify the role and molecular mechanism of LINC00174 in the progression of KIRC.
METHODS
UNASSIGNED
LINC00174 expression in KIRC and its prognostic value were analyzed by bioinformatics. LINC00174, miR-612 and FOXM1 mRNA expression levels in KIRC clinical samples and cell lines were detected by qRT-PCR. After LINC00174 was overexpressed or knocked down, CCK-8, BrdU and Transwell assays were adopted to evaluate the proliferation and metastatic potential of KIRC cells. Bioinformatics and dual luciferase reporter assays were employed to validate the targeting relationship between miR-612 and LINC00174 or FOXM1 mRNA, respectively. Western blot assay was performed to detect FOXM1 protein expression in KIRC cells.
RESULTS
UNASSIGNED
LINC00174 expression and FOXM1 expression were up-regulated in 42 cases of KIRC tissues (
CONCLUSION
UNASSIGNED
LINC00174 facilitates the proliferation and metastatic potential of KIRC cells
Identifiants
pubmed: 35616230
doi: 10.1080/08923973.2022.2082303
doi:
Substances chimiques
FOXM1 protein, human
0
Forkhead Box Protein M1
0
MIRN612 microRNA, human
0
MicroRNAs
0
RNA, Messenger
0
Bromodeoxyuridine
G34N38R2N1
Sincalide
M03GIQ7Z6P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM