Protoplast isolation and transient gene expression in different petunia cultivars.
PCR
Petunia cultivars
Protoplast isolation
Transfection efficiency
eGFP
Journal
Protoplasma
ISSN: 1615-6102
Titre abrégé: Protoplasma
Pays: Austria
ID NLM: 9806853
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
08
03
2022
accepted:
17
05
2022
pubmed:
28
5
2022
medline:
10
1
2023
entrez:
27
5
2022
Statut:
ppublish
Résumé
The protocol optimized for Petunia hybrida cv. Mirage Rose produced high protoplast yields in 3 out of other 11 cultivars (Damask White, Dreams White, and Opera Supreme White). Factors optimized in the protoplast transfection process showed that the best transfection efficiency (80%) was obtained using 2.5 × 10
Identifiants
pubmed: 35622155
doi: 10.1007/s00709-022-01776-9
pii: 10.1007/s00709-022-01776-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
271-280Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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