Target-enrichment sequencing yields valuable genomic data for challenging-to-culture bacteria of public health importance.
Bacillus anthracis
Mycoplasma amphoriforme
RNA baits
bacteria
genomic
target-enrichment sequencing
Journal
Microbial genomics
ISSN: 2057-5858
Titre abrégé: Microb Genom
Pays: England
ID NLM: 101671820
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
entrez:
27
5
2022
pubmed:
28
5
2022
medline:
1
6
2022
Statut:
ppublish
Résumé
Genomic data contribute invaluable information to the epidemiological investigation of pathogens of public health importance. However, whole-genome sequencing (WGS) of bacteria typically relies on culture, which represents a major hurdle for generating such data for a wide range of species for which culture is challenging. In this study, we assessed the use of culture-free target-enrichment sequencing as a method for generating genomic data for two bacterial species: (1)
Identifiants
pubmed: 35622897
doi: 10.1099/mgen.0.000836
pmc: PMC9465068
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_16045
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R012075/1
Pays : United Kingdom
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