SUMOylation does not affect cardiac troponin I stability but alters indirectly the development of force in response to Ca
SUMO
heart failure
myocytes
myofilaments
troponin I
Journal
The FEBS journal
ISSN: 1742-4658
Titre abrégé: FEBS J
Pays: England
ID NLM: 101229646
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
21
03
2022
received:
19
05
2021
accepted:
26
05
2022
pubmed:
29
5
2022
medline:
20
10
2022
entrez:
28
5
2022
Statut:
ppublish
Résumé
Post-translational modification of the myofilament protein troponin I by phosphorylation is known to trigger functional changes that support enhanced contraction and relaxation of the heart. We report for the first time that human troponin I can also be modified by SUMOylation at lysine 177. Functionally, TnI SUMOylation is not a factor in the development of passive and maximal force generation in response to calcium, however this modification seems to act indirectly by preventing SUMOylation of other myofilament proteins to alter calcium sensitivity and cooperativity of myofilaments. Utilising a novel, custom SUMO site-specific antibody that recognises only the SUMOylated form of troponin I, we verify that this modification occurs in human heart and that it is upregulated during disease.
Identifiants
pubmed: 35633070
doi: 10.1111/febs.16537
pmc: PMC9588612
mid: NIHMS1825797
doi:
Substances chimiques
Troponin I
0
Lysine
K3Z4F929H6
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
6267-6285Subventions
Organisme : British Heart Foundation
ID : PG/21/10363
Pays : United Kingdom
Organisme : NHLBI NIH HHS
ID : R01 HL148785
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL149164
Pays : United States
Organisme : British Heart Foundation
ID : FS/15/64/32035
Pays : United Kingdom
Informations de copyright
© 2022 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
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