Efficient monoacylglycerol synthesis by carboxylesterase EstGtA2 from Geobacillus thermodenitrificans in a solvent-free two-phase system.

Carboxylesterase / metabolism Enzymes, Immobilized Esterification Fatty Acids Geobacillus / genetics Glycerol Monoglycerides Solvents
Enzymatic monoacylglycerol synthesis Lipolytic enzyme Monoacylglycerol Solvent-free two-phase system Thermal-tolerant enzyme

Journal

Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800

Informations de publication

Date de publication:
Aug 2022
Historique:
received: 24 12 2021
revised: 25 03 2022
accepted: 05 05 2022
pubmed: 2 6 2022
medline: 5 8 2022
entrez: 1 6 2022
Statut: ppublish

Résumé

The present study investigated high-yield monoacylglycerol (MAG) synthesis by bacterial lipolytic enzymes in a solvent-free two-phase system. Esterification by monoacylglycerol lipase from Bacillus sp. H-257 (H257) required a high glycerol/fatty acid molar ratio for efficient MAG synthesis. Screening of H257 homologues revealed that carboxylesterase derived from Geobacillus thermodenitrificans, EstGtA2, exhibited a higher esterification rate than H257. Moreover, neutralizing the pH of the acidic reaction solution by adding potassium hydroxide (KOH) solution further increased the esterification rate. The esterification rate by EstGtA2 reached 75% under conditions of equivalent molar amounts of glycerol and fatty acid, and the MAG rate (MAG/total glyceride) was 97%. The neutralized pH of the reaction solution likely affected the thermal stability of EstGtA2 during the esterification reaction. Screening for thermal-tolerant variants revealed that the EstGtA2

Identifiants

DOI: 10.1016/j.jbiosc.2022.05.004 PMID: 35644798
pubmed: 35644798
pii: S1389-1723(22)00128-1
doi: 10.1016/j.jbiosc.2022.05.004
pii:
doi:

Substances chimiques

Enzymes, Immobilized 0
Fatty Acids 0
Monoglycerides 0
Solvents 0
Carboxylesterase EC 3.1.1.1
Glycerol PDC6A3C0OX

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

89-94

Informations de copyright

Copyright © 2022 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Auteurs

Tatsuya Osamura (T)

Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama 640-8580, Japan. Electronic address: osamura.tatsuya@kao.com.

Kyoshiro Nonaka (K)

Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama 640-8580, Japan.

Fumikazu Takahashi (F)

Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama 640-8580, Japan.

Mitsuyoshi Okuda (M)

Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan.

Hiroshi Hagihara (H)

Performance Chemical Research, Kao Corporation, 2-1-3, Bunka, Sumida-ku, Tokyo 131-8501, Japan.

Yasushi Takimura (Y)

Biological Science Research, Kao Corporation, 2606 Akabane, Ichikai, Haga, Tochigi 321-3497, Japan.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Carboxylic ester hydrolases › Carboxylesterase › Efficient monoacylglycerol synthesis by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines immobilisées › Enzymes immobilisées › Efficient monoacylglycerol synthesis by...
questionsmedicales.fr › Métabolisme › Estérification › Efficient monoacylglycerol synthesis by...
questionsmedicales.fr › Lipides › Acides gras › Efficient monoacylglycerol synthesis by...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Geobacillus › Efficient monoacylglycerol synthesis by...
questionsmedicales.fr › Glucides › Polyols › Dérivés du glycérol › Glycérol › Efficient monoacylglycerol synthesis by...
questionsmedicales.fr › Lipides › Glycérides › Monoglycérides › Efficient monoacylglycerol synthesis by...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Solvants › Efficient monoacylglycerol synthesis by...