Improving E. coli organic solvent tolerance by 1,4-dihydroxy-2-naphthoic acid.

Bacterial Proteins / metabolism Escherichia coli / genetics Escherichia coli Proteins / genetics Gene Expression Regulation, Bacterial Naphthols Solvents / metabolism Trans-Activators / genetics
Escherichia coli 1,4-dihydroxy-2-naphthoic acid AcrAB-TolC DHNA organic solvent

Journal

Bioscience, biotechnology, and biochemistry
ISSN: 1347-6947
Titre abrégé: Biosci Biotechnol Biochem
Pays: England
ID NLM: 9205717

Informations de publication

Date de publication:
22 Jul 2022
Historique:
received: 23 03 2022
accepted: 21 05 2022
pubmed: 2 6 2022
medline: 26 7 2022
entrez: 1 6 2022
Statut: ppublish

Résumé

Improving the organic solvent tolerance of bacteria is beneficial for the bioproduction of various valuable chemicals. In this study, we found that 1,4-dihydroxy-2-naphthoic acid (DHNA), known as a prebiotic, increased organic solvent tolerance in Escherichia coli. The AcrAB-TolC multidrug efflux pump contributes to the intrinsic organic solvent tolerance of E. coli. The addition of DHNA increased this pump's expression level. Transcriptional activators MarA, SoxS, and Rob proteins are known to control the expression of marA/soxS/rob regulon genes, including acrAB and tolC. Evaluation of the organic solvent tolerances of ΔmarA mutant, ΔsoxS mutant, and Δrob mutant showed that ΔmarA mutant and ΔsoxS mutant did not improve organic solvent tolerance by the addition of DHNA. In addition, DHNA increased the promoter activities of both marA and soxS. These results indicated that DHNA induces the AcrAB-TolC pump through both the marRAB system and the soxRS system.

Identifiants

DOI: 10.1093/bbb/zbac083 PMID: 35648476
pubmed: 35648476
pii: 6596869
doi: 10.1093/bbb/zbac083
doi:

Substances chimiques

Bacterial Proteins 0
Escherichia coli Proteins 0
Naphthols 0
Solvents 0
Trans-Activators 0
1,4-dihydroxy-2-naphthoic acid 1U77MR5Q7O

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1128-1135

Subventions

Organisme : Toyo University Top Priority Research Promotion Program
Organisme : Japan Society for the Promotion of Science
ID : 17K07731
Organisme : Programme for the Strategic Research Foundation at Private Universities
ID : S1101017
Organisme : MEXT

Informations de copyright

© The Author(s) 2022. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.

Auteurs

Noriyuki Doukyu (N)

Department of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Gunma, Japan.
Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Gunma, Japan.
Bio-Nano Electronic Research Center, Toyo University, 2100, Kujirai, Kawagoe, Saitama, Japan.
ORCID: 0000-0002-9392-9278

Hideyuki Fujisawa (H)

Graduate School of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Gunma, Japan.

Ryota Saito (R)

Department of Life Sciences, Toyo University, 1-1-1 Izumino, Itakura-machi, Gunma, Japan.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Improving E. coli organic solvent tolerance by...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Improving E. coli organic solvent tolerance by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › Improving E. coli organic solvent tolerance by...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes bactériens › Improving E. coli organic solvent tolerance by...
questionsmedicales.fr › Composés polycycliques › Hydrocarbures aromatiques polycycliques › Naphtalènes › Naphtols › Improving E. coli organic solvent tolerance by...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Solvants › Improving E. coli organic solvent tolerance by...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales régulatrices ou accessoires › Transactivateurs › Improving E. coli organic solvent tolerance by...