Caulobacter lipid A is conditionally dispensable in the absence of fur and in the presence of anionic sphingolipids.
CP: Microbiology
Caulobacter
Fur
ceramide
iron
lipid A
lipopolysaccharide
outer membrane
sphingolipid
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
31 05 2022
31 05 2022
Historique:
received:
25
01
2022
revised:
29
03
2022
accepted:
06
05
2022
entrez:
1
6
2022
pubmed:
2
6
2022
medline:
7
6
2022
Statut:
ppublish
Résumé
Lipid A, the membrane-anchored portion of lipopolysaccharide (LPS), is an essential component of the outer membrane (OM) of nearly all Gram-negative bacteria. Here we identify regulatory and structural factors that together render lipid A nonessential in Caulobacter crescentus. Mutations in the ferric uptake regulator fur allow Caulobacter to survive in the absence of either LpxC, which catalyzes an early step of lipid A synthesis, or CtpA, a tyrosine phosphatase homolog we find is needed for wild-type lipid A structure and abundance. Alterations in Fur-regulated processes, rather than iron status per se, underlie the ability to survive when lipid A synthesis is blocked. Fitness of lipid A-deficient Caulobacter requires an anionic sphingolipid, ceramide phosphoglycerate (CPG), which also mediates sensitivity to the antibiotic colistin. Our results demonstrate that, in an altered regulatory landscape, anionic sphingolipids can support the integrity of a lipid A-deficient OM.
Identifiants
pubmed: 35649364
pii: S2211-1247(22)00663-5
doi: 10.1016/j.celrep.2022.110888
pmc: PMC9393093
mid: NIHMS1812257
pii:
doi:
Substances chimiques
Lipid A
0
Lipopolysaccharides
0
Sphingolipids
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
110888Subventions
Organisme : NIAID NIH HHS
ID : R01 AI123820
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI147314
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM111066
Pays : United States
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests J.J.Z. and K.R.R. have applied for US (62/914,271) and international (PCT/US20/28,464) patents on lipid A-deficient Caulobacter.
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