Parameters Optimization for Improving Bioluminescence Inhibition Assay Using Vibrio fischeri Bacteria to Detect Lipopolysaccharide Toxicity in Aquatic Environments.


Journal

Bulletin of environmental contamination and toxicology
ISSN: 1432-0800
Titre abrégé: Bull Environ Contam Toxicol
Pays: United States
ID NLM: 0046021

Informations de publication

Date de publication:
28 Oct 2024
Historique:
received: 05 06 2024
accepted: 15 10 2024
medline: 28 10 2024
pubmed: 28 10 2024
entrez: 28 10 2024
Statut: epublish

Résumé

Bioluminescence inhibition of Vibrio fischeri is a widely used method for toxicity testing in aquatic environments. Certain complex biological contaminants, such as lipopolysaccharide (LPS), can interfere with metabolic pathways during toxicity assays. The standard 15-minute Vibrio fischeri bioluminescence assay has limitations when evaluating and screening water toxicity against complex and emerging chemicals like LPS. To accurately determine the effects of such substances, it is crucial to use a bioassay that encompasses a sufficient cell cycle period. This study tested LPS at varying incubation times (ranging from 60 s to 60 min) and concentrations (1-1*10

Identifiants

pubmed: 39466431
doi: 10.1007/s00128-024-03970-2
pii: 10.1007/s00128-024-03970-2
doi:

Substances chimiques

Lipopolysaccharides 0
Water Pollutants, Chemical 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

61

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Reyhaneh Dehghan (R)

Department of Biodiversity and Ecosystem Management, Environmental Sciences Research Institute, Shahid Beheshti University, Tehran, Iran.

Khosro Piri (K)

Department of Biodiversity and Ecosystem Management, Environmental Sciences Research Institute, Shahid Beheshti University, Tehran, Iran. p_khosro@sbu.ac.ir.

Asghar Abdoli (A)

Department of Biodiversity and Ecosystem Management, Environmental Sciences Research Institute, Shahid Beheshti University, Tehran, Iran.

Saman Hosseinkhani (S)

Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

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