Detection of cell-cell interactions via photocatalytic cell tagging.
Journal
Nature chemical biology
ISSN: 1552-4469
Titre abrégé: Nat Chem Biol
Pays: United States
ID NLM: 101231976
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
received:
29
01
2022
accepted:
22
04
2022
pubmed:
3
6
2022
medline:
3
8
2022
entrez:
2
6
2022
Statut:
ppublish
Résumé
The growing appreciation of immune cell-cell interactions within disease environments has led to extensive efforts to develop immunotherapies. However, characterizing complex cell-cell interfaces in high resolution remains challenging. Thus, technologies leveraging therapeutic-based modalities to profile intercellular environments offer opportunities to study cell-cell interactions with molecular-level insight. We introduce photocatalytic cell tagging (PhoTag) for interrogating cell-cell interactions using single-domain antibodies (VHHs) conjugated to photoactivatable flavin-based cofactors. Following irradiation with visible light, the flavin photocatalyst generates phenoxy radical tags for targeted labeling. Using this technology, we demonstrate selective synaptic labeling across the PD-1/PD-L1 axis in antigen-presenting cell-T cell systems. In combination with multiomics single-cell sequencing, we monitored interactions between peripheral blood mononuclear cells and Raji PD-L1 B cells, revealing differences in transient interactions with specific T cell subtypes. The utility of PhoTag in capturing cell-cell interactions will enable detailed profiling of intercellular communication across different biological systems.
Identifiants
pubmed: 35654846
doi: 10.1038/s41589-022-01044-0
pii: 10.1038/s41589-022-01044-0
doi:
Substances chimiques
B7-H1 Antigen
0
Flavins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
850-858Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.
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