Quantitative Measurement of Ascorbate and Glutathione by Spectrophotometry.
Antioxidant
Ascorbate
Glutathione
H2O2
ROS
Redox state
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
3
6
2022
pubmed:
4
6
2022
medline:
9
6
2022
Statut:
ppublish
Résumé
Ascorbate and glutathione are key chemical antioxidants present at relatively high concentrations in plant cells. They are also reducing cofactors for enzymes that process hydrogen peroxide in the ascorbate-glutathione pathway. Due to these two related biochemical functions, the compounds form an interface between reactive oxygen species and sensitive cellular components. Therefore, their status can provide reliable and direct information on cell redox state, signaling, and plant health. While several methods exist for quantification of ascorbate and glutathione, simple enzyme-dependent assays allow them to be measured easily and inexpensively in common extracts. This chapter describes a protocol to measure total contents, as well as the major oxidized and reduced forms, of both compounds in plant tissues.
Identifiants
pubmed: 35657513
doi: 10.1007/978-1-0716-2469-2_6
doi:
Substances chimiques
Antioxidants
0
Hydrogen Peroxide
BBX060AN9V
Ascorbate Peroxidases
EC 1.11.1.11
Glutathione
GAN16C9B8O
Ascorbic Acid
PQ6CK8PD0R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
87-96Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
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