Exposure of human cerebral microvascular endothelial cells hCMEC/D3 to laminar shear stress induces vascular protective responses.
Blood–brain barrier
Brain microvessels
Human endothelial cells
Proteomics
Shear stress
Journal
Fluids and barriers of the CNS
ISSN: 2045-8118
Titre abrégé: Fluids Barriers CNS
Pays: England
ID NLM: 101553157
Informations de publication
Date de publication:
03 Jun 2022
03 Jun 2022
Historique:
received:
20
12
2021
accepted:
23
05
2022
entrez:
6
6
2022
pubmed:
7
6
2022
medline:
9
6
2022
Statut:
epublish
Résumé
Endothelial cells (ECs) are constantly submitted in vivo to hemodynamical forces derived from the blood circulation, including shear stress (SS). ECs are able to detect SS and consequently adapt their phenotype, thus affecting many endothelial functions. If a plethora of shear stress-regulated molecular networks have been described in peripheral ECs, less is known about the molecular responses of microvascular brain ECs which constitute the blood-brain barrier (BBB). In this work, we investigated the response of human cerebral microvascular ECs to laminar physiological shear stress using the well characterized hCMEC/D3 cell line. Interestingly, we showed that hCMEC/D3 cells responded to shear stress by aligning perpendicularly to the flow direction, contrary to peripheral endothelial cells which aligned in the flow direction. Whole proteomic profiles were compared between hCMEC/D3 cells cultured either in static condition or under 5 or 10 dyn.cm
Identifiants
pubmed: 35658915
doi: 10.1186/s12987-022-00344-w
pii: 10.1186/s12987-022-00344-w
pmc: PMC9164338
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
41Informations de copyright
© 2022. The Author(s).
Références
FASEB J. 2007 Nov;21(13):3553-61
pubmed: 17557931
Proc Natl Acad Sci U S A. 2001 Apr 10;98(8):4478-85
pubmed: 11296290
Sci Rep. 2019 Mar 18;9(1):4738
pubmed: 30894565
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Nat Commun. 2018 Aug 29;9(1):3506
pubmed: 30158636
Vasc Endovascular Surg. 2003 Jan-Feb;37(1):47-57
pubmed: 12577139
Nat Commun. 2020 Jan 10;11(1):175
pubmed: 31924752
Cardiovasc Res. 2007 Jun 1;74(3):497-505
pubmed: 17383622
J Cell Sci. 1996 Apr;109 ( Pt 4):713-26
pubmed: 8718663
FASEB J. 2022 May;36(5):e22278
pubmed: 35436025
Curr Drug Targets. 2010 Dec;11(12):1517-31
pubmed: 20704549
Expert Rev Proteomics. 2014 Oct;11(5):611-9
pubmed: 25017810
EMBO J. 2001 Sep 3;20(17):4639-47
pubmed: 11532928
Blood. 2002 Sep 1;100(5):1689-98
pubmed: 12176889
FASEB J. 2005 Nov;19(13):1872-4
pubmed: 16141364
Antioxid Redox Signal. 2017 Nov 1;27(13):989-1010
pubmed: 28443683
J Neurochem. 2017 Aug;142(4):597-609
pubmed: 28581635
Front Physiol. 2017 Aug 24;8:595
pubmed: 28883796
Circ Res. 2000 Oct 13;87(8):683-9
pubmed: 11029404
Biochem Pharmacol. 2009 Mar 1;77(5):897-909
pubmed: 19041851
Metabolites. 2020 Jul 17;10(7):
pubmed: 32709039
Cell Mol Bioeng. 2016 Mar;9(1):127-138
pubmed: 28989541
J Proteome Res. 2016 Mar 4;15(3):777-87
pubmed: 26653327
Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):8955-60
pubmed: 11481467
Mol Med Rep. 2019 Mar;19(3):1739-1746
pubmed: 30628700
J Immunol. 2010 Jul 1;185(1):569-77
pubmed: 20511556
Microvasc Res. 2015 May;99:8-18
pubmed: 25725258
Sci Adv. 2017 Nov 08;3(11):e1701679
pubmed: 29134197
Bioeng Transl Med. 2019 Jan 13;4(2):e10126
pubmed: 31249876
J Clin Invest. 2006 Jan;116(1):49-58
pubmed: 16341264
BMC Neurosci. 2011 May 11;12:40
pubmed: 21569296
Physiol Rev. 2006 Oct;86(4):1179-236
pubmed: 17015488
Microvasc Res. 2020 Mar;128:103930
pubmed: 31639383
Nat Methods. 2014 Mar;11(3):319-24
pubmed: 24487582
Mol Pharm. 2013 Jan 7;10(1):289-96
pubmed: 23137377
Fluids Barriers CNS. 2016 Sep 21;13(1):17
pubmed: 27655189
Mol Cell Biol. 2011 Sep;31(18):3845-56
pubmed: 21746876
Exp Eye Res. 1977;25 Suppl:1-25
pubmed: 73470
Nat Commun. 2012 Mar 06;3:718
pubmed: 22395609
Mol Pharm. 2010 Aug 2;7(4):1057-68
pubmed: 20524699
Int J Mol Sci. 2019 May 16;20(10):
pubmed: 31100781
Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):10990-10995
pubmed: 28973892
Arteriosclerosis. 1986 Mar-Apr;6(2):220-9
pubmed: 3954676
Exp Mol Pathol. 1980 Jun;32(3):276-89
pubmed: 7379981
J Biomech Eng. 1981 Aug;103(3):172-6
pubmed: 7278195
J Cereb Blood Flow Metab. 2017 Jul;37(7):2614-2625
pubmed: 27702879
Neuroscience. 2004;129(2):349-60
pubmed: 15501592
Biorheology. 2007;44(5-6):375-86
pubmed: 18401076
Cold Spring Harb Perspect Biol. 2015 Jan 05;7(1):a020412
pubmed: 25561720
Thorax. 1980 Mar;35(3):186-91
pubmed: 7385090
Ann Biomed Eng. 2020 Feb;48(2):780-793
pubmed: 31741228
J Cell Physiol. 2011 Nov;226(11):3053-63
pubmed: 21302304
J Biol Chem. 2003 Jan 10;278(2):703-11
pubmed: 12370194
J Neurochem. 2011 Apr;117(2):333-45
pubmed: 21291474
J Atheroscler Thromb. 2003;10(5):304-13
pubmed: 14718748
Am J Physiol Heart Circ Physiol. 2007 Jun;292(6):H3190-7
pubmed: 17308001
PLoS One. 2012;7(5):e38149
pubmed: 22675443
J Biomed Sci. 2009 Jan 22;16:12
pubmed: 19272177
Proteomics. 2015 Apr;15(8):1453-6
pubmed: 25644178
Nat Genet. 2003 Jul;34(3):267-73
pubmed: 12808457
Nat Clin Pract Cardiovasc Med. 2009 Jan;6(1):16-26
pubmed: 19029993
Blood. 2006 Jun 1;107(11):4354-63
pubmed: 16455954
J Neurochem. 2008 Dec;107(6):1518-28
pubmed: 19094056
J Biomech Eng. 1985 Nov;107(4):341-7
pubmed: 4079361
Biochem Biophys Res Commun. 2018 Jul 20;502(3):403-408
pubmed: 29852173
J Cell Sci. 2004 May 15;117(Pt 12):2427-9
pubmed: 15159447
Int J Hypertens. 2012;2012:127910
pubmed: 22518279
DNA Cell Biol. 2017 May;36(5):335-346
pubmed: 28287831
Arteriosclerosis. 1987 May-Jun;7(3):276-86
pubmed: 3593075
Sci Rep. 2014 Apr 15;4:4681
pubmed: 24732421
Appl Environ Microbiol. 1995 Apr;61(4):1614-9
pubmed: 7747975
Endothelium. 2002;9(2):89-102
pubmed: 12200960
Brain Res. 2007 May 25;1147:39-50
pubmed: 17368578
J Exp Med. 2006 Aug 7;203(8):1939-50
pubmed: 16864659
J Cereb Blood Flow Metab. 2021 Sep;41(9):2201-2215
pubmed: 33563079
Biomaterials. 2019 Jan;190-191:24-37
pubmed: 30391800
Arterioscler Thromb Vasc Biol. 2017 Nov;37(11):2087-2101
pubmed: 28882872
J Clin Med. 2019 Jan 24;8(2):
pubmed: 30682787
Antioxid Redox Signal. 2011 Sep 1;15(5):1405-14
pubmed: 21050140
Tissue Eng Part A. 2012 Jan;18(1-2):127-36
pubmed: 21787250
EMBO J. 2002 Dec 16;21(24):6791-800
pubmed: 12486000
Am J Physiol Cell Physiol. 2006 Feb;290(2):C444-52
pubmed: 16176968
Front Neuroeng. 2013 Aug 30;6:7
pubmed: 24009582
Hypertension. 2011 Aug;58(2):225-31
pubmed: 21690482
Fluids Barriers CNS. 2017 Aug 4;14(1):20
pubmed: 28774343
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Toxicol In Vitro. 2005 Apr;19(3):299-334
pubmed: 15713540
Sci Rep. 2020 Mar 2;10(1):3788
pubmed: 32123236
Mol Biol Cell. 2011 Nov;22(22):4324-34
pubmed: 21937718
Compr Physiol. 2019 Mar 15;9(2):873-904
pubmed: 30873580
J Pharm Biomed Anal. 2019 Feb 5;164:496-508
pubmed: 30453156
Int J Mol Sci. 2020 Jul 06;21(13):
pubmed: 32640524
Physiol Rev. 1995 Jul;75(3):519-60
pubmed: 7624393
Cell Stem Cell. 2019 Jun 6;24(6):995-1005.e6
pubmed: 31173718
Fluids Barriers CNS. 2013 Mar 26;10(1):16
pubmed: 23531482
Ann Biomed Eng. 2013 Jul;41(7):1411-27
pubmed: 23229281