Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: a comparative study.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 06 2022
03 06 2022
Historique:
received:
14
02
2022
accepted:
18
05
2022
entrez:
6
6
2022
pubmed:
7
6
2022
medline:
9
6
2022
Statut:
epublish
Résumé
MPO-derived oxidants including HOCl contribute to tissue damage and the initiation and propagation of inflammatory diseases. The search for small molecule inhibitors of myeloperoxidase, as molecular tools and potential drugs, requires the application of high throughput screening assays based on monitoring the activity of myeloperoxidase. In this study, we have compared three classes of fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid, including boronate-, aminophenyl- and thiol-based fluorogenic probes and we show that all three classes of probes are suitable for this purpose. However, probes based on the coumarin fluorophore turned out to be not reliable indicators of the inhibitors' potency. We have also determined the rate constants of the reaction between HOCl and the probes and they are equal to 1.8 × 10
Identifiants
pubmed: 35660769
doi: 10.1038/s41598-022-13317-8
pii: 10.1038/s41598-022-13317-8
pmc: PMC9166712
doi:
Substances chimiques
Boronic Acids
0
Coumarins
0
Fluoresceins
0
Fluorescent Dyes
0
Hypochlorous Acid
712K4CDC10
Peroxidase
EC 1.11.1.7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9314Informations de copyright
© 2022. The Author(s).
Références
Free Radic Biol Med. 2014 May;70:96-105
pubmed: 24566469
Spectrochim Acta A Mol Biomol Spectrosc. 2016 Sep 5;166:79-83
pubmed: 27214272
J Biol Chem. 2012 Aug 10;287(33):27913-23
pubmed: 22718769
J Biol Chem. 2016 Mar 25;291(13):7029-44
pubmed: 26839313
J Biol Chem. 2003 Jan 31;278(5):3170-5
pubmed: 12419811
Front Chem. 2020 Sep 25;8:580899
pubmed: 33102447
J Am Chem Soc. 2010 Apr 28;132(16):5906-15
pubmed: 20361787
Antioxid Redox Signal. 2020 May 1;32(13):957-981
pubmed: 31989833
Free Radic Biol Med. 2016 Jun;95:323-32
pubmed: 27021961
Free Radic Biol Med. 2014 Feb;67:278-84
pubmed: 24200598
Br J Pharmacol. 2007 Nov;152(6):838-54
pubmed: 17592500
J Biomol Screen. 1999;4(2):67-73
pubmed: 10838414
Cell Biochem Biophys. 2017 Dec;75(3-4):335-349
pubmed: 28660426
Arch Biochem Biophys. 2000 May 1;377(1):95-100
pubmed: 10775446
Free Radic Biol Med. 2016 Dec;101:284-295
pubmed: 27641237
FEBS Lett. 1996 Jan 22;379(1):103-6
pubmed: 8566218
Biochem Pharmacol. 2010 Apr 15;79(8):1156-64
pubmed: 19968966
Talanta. 2021 May 1;226:122152
pubmed: 33676701
J Biol Chem. 2010 May 7;285(19):14210-6
pubmed: 20194496
J Biol Chem. 2012 Jan 27;287(5):2984-95
pubmed: 22139901
Antioxid Redox Signal. 2018 May 20;28(15):1416-1432
pubmed: 29037049
Anal Chem. 2018 Jan 2;90(1):533-555
pubmed: 29056044
Biochem J. 2011 Nov 1;439(3):423-31
pubmed: 21749327
Methods Enzymol. 1994;233:502-12
pubmed: 8015486
Neuroreport. 2018 Feb 7;29(3):208-213
pubmed: 29266034
Acc Chem Res. 2019 Aug 20;52(8):2158-2168
pubmed: 31318529
J Lipid Res. 2013 Nov;54(11):3016-29
pubmed: 23883583
J Biol Chem. 2013 Dec 20;288(51):36636-47
pubmed: 24194519
Pharmacol Rep. 2015 Aug;67(4):756-64
pubmed: 26321278
Pharmacol Ther. 2021 Feb;218:107685
pubmed: 32961264
J Neuroinflammation. 2016 May 24;13(1):119
pubmed: 27220420
Free Radic Biol Med. 2009 Nov 15;47(10):1401-7
pubmed: 19686842
Biochem J. 2009 Jun 12;421(1):79-86
pubmed: 19379130
Free Radic Biol Med. 2016 Oct;99:32-42
pubmed: 27458121
J Biol Chem. 1985 Apr 10;260(7):4003-6
pubmed: 2984193
J Biol Chem. 2014 Aug 8;289(32):22536-53
pubmed: 24928516
Arch Biochem Biophys. 1995 Oct 20;323(1):120-6
pubmed: 7487057
Tetrahedron Lett. 2008 May 5;49(19):3045-3048
pubmed: 19081820
Free Radic Biol Med. 2014 Aug;73:60-6
pubmed: 24794410
Chem Res Toxicol. 2001 Oct;14(10):1453-64
pubmed: 11599938
Chem Res Toxicol. 2016 May 16;29(5):735-46
pubmed: 27081868
Free Radic Res. 2015 Jun;49(6):768-76
pubmed: 25536418
Atherosclerosis. 2015 May;240(1):234-41
pubmed: 25818249
Free Radic Biol Med. 2005 Dec 1;39(11):1468-77
pubmed: 16274882
J Pharmacol Exp Ther. 2018 Feb;364(2):311-322
pubmed: 29255000
Antioxid Redox Signal. 2008 Jul;10(7):1199-234
pubmed: 18331199
Chem Res Toxicol. 2012 Sep 17;25(9):1793-9
pubmed: 22731669
Chem Sci. 2019 May 24;10(24):6035-6071
pubmed: 31360411
Pharmacol Ther. 2021 May;221:107711
pubmed: 33137376
J Biol Chem. 1999 Apr 2;274(14):9494-502
pubmed: 10092633
Free Radic Biol Med. 2013 Jan;54:135-47
pubmed: 23051008
J Biol Chem. 2014 Jun 6;289(23):16176-89
pubmed: 24764302
Chem Asian J. 2019 Sep 16;14(18):3048-3084
pubmed: 31347256
J Mater Chem B. 2018 Mar 28;6(12):1716-1733
pubmed: 32254244