HGprt deficiency disrupts dopaminergic circuit development in a genetic mouse model of Lesch-Nyhan disease.


Journal

Cellular and molecular life sciences : CMLS
ISSN: 1420-9071
Titre abrégé: Cell Mol Life Sci
Pays: Switzerland
ID NLM: 9705402

Informations de publication

Date de publication:
04 Jun 2022
Historique:
received: 17 02 2022
accepted: 23 04 2022
revised: 05 04 2022
entrez: 6 6 2022
pubmed: 7 6 2022
medline: 9 6 2022
Statut: epublish

Résumé

In Lesch-Nyhan disease (LND), deficiency of the purine salvage enzyme hypoxanthine guanine phosphoribosyl transferase (HGprt) leads to a characteristic neurobehavioral phenotype dominated by dystonia, cognitive deficits and incapacitating self-injurious behavior. It has been known for decades that LND is associated with dysfunction of midbrain dopamine neurons, without overt structural brain abnormalities. Emerging post mortem and in vitro evidence supports the hypothesis that the dopaminergic dysfunction in LND is of developmental origin, but specific pathogenic mechanisms have not been revealed. In the current study, HGprt deficiency causes specific neurodevelopmental abnormalities in mice during embryogenesis, particularly affecting proliferation and migration of developing midbrain dopamine (mDA) neurons. In mutant embryos at E14.5, proliferation was increased, accompanied by a decrease in cell cycle exit and the distribution and orientation of dividing cells suggested a premature deviation from their migratory route. An abnormally structured radial glia-like scaffold supporting this mDA neuronal migration might lie at the basis of these abnormalities. Consequently, these abnormalities were associated with an increase in area occupied by TH

Identifiants

pubmed: 35660973
doi: 10.1007/s00018-022-04326-x
pii: 10.1007/s00018-022-04326-x
pmc: PMC9167210
doi:

Substances chimiques

Hypoxanthine Phosphoribosyltransferase EC 2.4.2.8
Dopamine VTD58H1Z2X

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

341

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS119758
Pays : United States
Organisme : Dutch Research Council
ID : 916.12.167
Pays : Netherlands
Organisme : Dutch Brain Foundation
ID : F2014(1)-16
Organisme : NWO - NWA-ORC
ID : NWA.1160.18.320

Informations de copyright

© 2022. The Author(s).

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Auteurs

J S Witteveen (JS)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.

S R Loopstok (SR)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.

L Luque Ballesteros (LL)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.

A Boonstra (A)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.

N H M van Bakel (NHM)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.
Department of Molecular Neurobiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.

W H P van Boekel (WHP)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.

G J M Martens (GJM)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.

J E Visser (JE)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.
Department of Molecular Neurobiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands.
Department of Neurology, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, Nijmegen, The Netherlands.
Department of Neurology, Amphia Hospital, Breda, The Netherlands.

S M Kolk (SM)

Department of Molecular Animal Physiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands. s.kolk@donders.ru.nl.
Department of Molecular Neurobiology, Donders Center for Neuroscience, Radboud University Nijmegen, Nijmegen, The Netherlands. s.kolk@donders.ru.nl.

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