Isolation of Decidual Macrophages and Hofbauer Cells from Term Placenta-Comparison of the Expression of CD163 and CD80.
Hofbauer cells
decidual macrophages
macrophage isolation protocol
placental macrophages
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
30 May 2022
30 May 2022
Historique:
received:
26
04
2022
revised:
26
05
2022
accepted:
27
05
2022
entrez:
10
6
2022
pubmed:
11
6
2022
medline:
14
6
2022
Statut:
epublish
Résumé
(1) Background: Placental immune cells are playing a very important role in a successful placentation and the prevention of pregnancy complications. Macrophages dominate in number and relevance in the maternal and the fetal part of the placenta. The evidence on the polarization state of fetal and maternal macrophages involved in both, healthy and pregnancy-associated diseases, is limited. There is no representative isolation method for the direct comparison of maternal and fetal macrophages so far. (2) Material and Methods: For the isolation of decidual macrophages and Hofbauer cells from term placenta, fresh tissue was mechanically dissected and digested with trypsin and collagenase A. Afterwards cell enrichment was increased by a Percoll gradient. CD68 is represented as pan-macrophage marker, the surface markers CD80 and CD163 were further investigated. (3) Results: The established method revealed a high cell yield and purity of the isolated macrophages and enabled the comparison between decidual macrophages and Hofbauer cells. No significant difference was observed in the percentage of single CD163
Identifiants
pubmed: 35682791
pii: ijms23116113
doi: 10.3390/ijms23116113
pmc: PMC9181726
pii:
doi:
Substances chimiques
Antigens, CD
0
Antigens, Differentiation, Myelomonocytic
0
B7-1 Antigen
0
CD163 antigen
0
Cell Adhesion Molecules
0
Receptors, Cell Surface
0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : DFG - funded Clinician Scientist Program PRIME
ID : 413635475
Références
Biol Reprod. 2013 Jun 20;88(6):155
pubmed: 23553431
Clin Exp Immunol. 2003 Mar;131(3):498-505
pubmed: 12605704
Oncogene. 2009 Apr 9;28(14):1706-13
pubmed: 19234485
Immunol Invest. 2008;37(5):535-64
pubmed: 18716937
Am J Pathol. 2009 May;174(5):1959-71
pubmed: 19349361
Placenta. 2021 Jan 15;104:277-283
pubmed: 33472135
Am J Pathol. 2003 Mar;162(3):887-96
pubmed: 12598322
Biol Reprod. 2003 Nov;69(5):1458-71
pubmed: 12890718
J Pathol Bacteriol. 1967 Apr;93(2):710-7
pubmed: 6054069
Differentiation. 1994 Jun;57(1):51-61
pubmed: 7915242
Am J Pathol. 2011 Feb;178(2):709-17
pubmed: 21281803
Am J Pathol. 2010 Aug;177(2):1017-30
pubmed: 20558572
J Reprod Immunol. 2009 Oct;82(1):24-31
pubmed: 19732959
J Clin Invest. 2006 Oct;116(10):2643-52
pubmed: 16998586
Wiad Lek. 2021;74(2):213-219
pubmed: 33813474
J Reprod Immunol. 2017 Nov;124:44-53
pubmed: 29055791
Placenta. 2010 Jun;31(6):535-44
pubmed: 20347485
Mol Hum Reprod. 2022 Mar 8;28(3):
pubmed: 35258594
Blood. 2008 Mar 15;111(6):3108-15
pubmed: 18187664
PLoS One. 2011;6(12):e29590
pubmed: 22216324
Immunology. 1983 May;49(1):103-12
pubmed: 6220962
Am J Reprod Immunol. 2004 Apr;51(4):275-82
pubmed: 15212680
Reproduction. 2012 May;143(5):713-25
pubmed: 22454532
J Leukoc Biol. 1999 Sep;66(3):391-400
pubmed: 10496308
Am J Reprod Immunol. 2003 Dec;50(6):444-52
pubmed: 14750551
J Immunol. 2011 Feb 15;186(4):2633-42
pubmed: 21257965
Front Immunol. 2018 Nov 15;9:2628
pubmed: 30498493
Proc Natl Acad Sci U S A. 2013 Jul 2;110(27):11109-14
pubmed: 23776237
J Leukoc Biol. 2002 Mar;71(3):445-57
pubmed: 11867682
Pharmacogenomics J. 2014 Dec;14(6):535-41
pubmed: 24913092
Cell Host Microbe. 2016 Jul 13;20(1):83-90
pubmed: 27247001
Annu Rev Physiol. 2017 Feb 10;79:541-566
pubmed: 27813830
Am J Reprod Immunol. 2015 Aug;74(2):100-9
pubmed: 25582625
Hum Reprod Update. 2016 Dec;23(1):77-103
pubmed: 27591247
Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7212-E7221
pubmed: 27807143
Curr Protoc Immunol. 2019 Jun;125(1):e77
pubmed: 31125190
Placenta. 2021 Jan 1;103:94-103
pubmed: 33120051
Int J Mol Sci. 2018 Jun 26;19(7):
pubmed: 29949879
Biochim Biophys Acta. 2013 Dec;1832(12):1959-68
pubmed: 23872577
J Exp Med. 2021 Jan 4;218(1):
pubmed: 33075123
Front Immunol. 2017 Jul 31;8:888
pubmed: 28824621
J Immunol. 2005 Feb 15;174(4):1820-9
pubmed: 15699108
Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3735-9
pubmed: 8097324
Int J Mol Sci. 2019 Jul 28;20(15):
pubmed: 31357698
Am J Reprod Immunol. 2010 Jun;63(6):434-44
pubmed: 20055791
J Immunol. 2011 Oct 1;187(7):3671-82
pubmed: 21890660
Am J Reprod Immunol. 2011 Oct;66(4):336-48
pubmed: 21545365
Eur J Obstet Gynecol Reprod Biol. 2020 May;248:211-221
pubmed: 32240894
Circ Res. 2016 Jun 24;119(1):55-68
pubmed: 27199465
Reproduction. 2016 Nov;152(5):447-55
pubmed: 27534571
Int J Dev Biol. 2010;54(2-3):281-94
pubmed: 19876837
Front Immunol. 2015 May 05;6:212
pubmed: 25999950
Front Microbiol. 2019 Oct 29;10:2434
pubmed: 31749776
J Reprod Immunol. 2021 Feb;143:103264
pubmed: 33360717
Retrovirology. 2012 Dec 05;9:101
pubmed: 23217137
Ann N Y Acad Sci. 2011 Mar;1221:80-7
pubmed: 21401634
Immunol Rev. 2022 Jul;308(1):40-54
pubmed: 35234305
J Immunol. 2008 Feb 15;180(4):2367-75
pubmed: 18250446
Cell Immunol. 2019 Feb;336:75-82
pubmed: 30665661
Front Immunol. 2017 Feb 09;8:120
pubmed: 28232836
Mt Sinai J Med. 2010 Mar-Apr;77(2):225-35
pubmed: 20309920
Immunol Rev. 2022 Jul;308(1):77-92
pubmed: 35451089
Am J Reprod Immunol. 2016 Mar;75(3):298-309
pubmed: 26750089
FASEB J. 2022 Jan;36(1):e22073
pubmed: 34847253
Am J Reprod Immunol. 2007 Aug;58(2):129-37
pubmed: 17631006
Int J Mol Sci. 2019 Jun 11;20(11):
pubmed: 31212604
Am J Reprod Immunol. 2013 Aug;70(2):104-15
pubmed: 23480364
J Reprod Immunol. 2011 Dec;92(1-2):97-102
pubmed: 22015003
J Reprod Immunol. 2022 Jun;151:103627
pubmed: 35468528
Tissue Antigens. 2003 Aug;62(2):104-16
pubmed: 12889991
Nat Rev Immunol. 2005 Dec;5(12):953-64
pubmed: 16322748
Sci Immunol. 2019 Jan 11;4(31):
pubmed: 30635356
Placenta. 2008 Mar;29(3):274-81
pubmed: 18262644
Placenta. 2014 Dec;35(12):1027-34
pubmed: 25449030
Front Immunol. 2019 Apr 15;10:792
pubmed: 31037072
J Reprod Immunol. 2017 Nov;124:30-37
pubmed: 29049918
J Cell Physiol. 2011 Aug;226(8):2139-49
pubmed: 21520066
J Am Soc Nephrol. 2017 Jul;28(7):2053-2067
pubmed: 28209809
Front Immunol. 2022 Mar 21;13:837391
pubmed: 35386706
Inflamm Res. 2001 Mar;50(3):123-8
pubmed: 11339499