PEAK1 Y635 phosphorylation regulates cell migration through association with Tensin3 and integrins.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
01 08 2022
01 08 2022
Historique:
received:
05
08
2021
revised:
22
03
2022
accepted:
18
05
2022
entrez:
10
6
2022
pubmed:
11
6
2022
medline:
15
6
2022
Statut:
ppublish
Résumé
Integrins mediate cell adhesion by connecting the extracellular matrix to the intracellular cytoskeleton and orchestrate signal transduction in response to chemical and mechanical stimuli by interacting with many cytoplasmic proteins. We used BioID to interrogate the interactomes of β1 and β3 integrins in epithelial cells and identified PEAK1 as an interactor of the RGD-binding integrins α5β1, αVβ3, and αVβ5 in focal adhesions. We demonstrate that the interaction between integrins and PEAK1 occurs indirectly through Tensin3, requiring both the membrane-proximal NPxY motif on the integrin β tail and binding of the SH2 domain of Tensin3 to phosphorylated Tyr-635 on PEAK1. Phosphorylation of Tyr-635 is mediated by Src and regulates cell migration. Additionally, we found that Shc1 localizes in focal adhesions in a PEAK1 phosphorylated Tyr-1188-dependent fashion. Besides binding Shc1, PEAK1 also associates with a protein cluster that mediates late EGFR/Shc1 signaling. We propose a model in which PEAK1 binds Tensin3 and Shc1 to converge integrin and growth factor receptor signal transduction.
Identifiants
pubmed: 35687021
pii: 213273
doi: 10.1083/jcb.202108027
pmc: PMC9194829
pii:
doi:
Substances chimiques
Integrin beta3
0
Integrins
0
TNS3 protein, human
0
Tensins
0
PEAK1 protein, human
EC 2.7.10.1
Protein-Tyrosine Kinases
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Dutch Cancer Society
ID : project 12143
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
Organisme : Alexander von Humboldt Foundation
Informations de copyright
© 2022 Zuidema et al.
Références
Sci Signal. 2022 Feb 22;15(722):eabj3554
pubmed: 35192416
ACS Chem Biol. 2007 Feb 20;2(2):109-18
pubmed: 17256997
EMBO Rep. 2011 Mar;12(3):259-66
pubmed: 21311561
J Biol Chem. 2013 Jan 4;288(1):123-31
pubmed: 23105102
Nat Commun. 2015 Feb 13;6:6265
pubmed: 25677187
Nat Commun. 2015 Sep 08;6:8320
pubmed: 26346464
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Nat Cell Biol. 2016 Sep;18(9):941-53
pubmed: 27548916
Sci Signal. 2016 Jun 14;9(432):rs4
pubmed: 27303058
Nat Cell Biol. 2013 Jun;15(6):625-36
pubmed: 23708002
Biochem J. 1997 Dec 1;328 ( Pt 2):529-37
pubmed: 9371712
J Proteome Res. 2011 Mar 4;10(3):1343-52
pubmed: 21214269
Exp Cell Res. 1999 Nov 1;252(2):439-48
pubmed: 10527634
FEBS J. 2020 Oct;287(19):4183-4197
pubmed: 31599110
J Cell Biol. 2007 Jan 1;176(1):43-9
pubmed: 17190795
J Cell Sci. 2018 Nov 5;131(21):
pubmed: 30301780
Mol Cell Biol. 2006 Nov;26(22):8461-74
pubmed: 16982700
Nat Cell Biol. 2015 Dec;17(12):1577-1587
pubmed: 26479319
Protein Sci. 2007 Jun;16(6):1223-9
pubmed: 17473008
J Biol Chem. 2000 Nov 17;275(46):36423-9
pubmed: 10964917
Cancer Res. 2017 Apr 15;77(8):1997-2007
pubmed: 28381547
Cold Spring Harb Perspect Biol. 2011 May 01;3(5):
pubmed: 21441590
Cancer Cell. 2009 Sep 8;16(3):246-58
pubmed: 19732724
J Cell Sci. 2014 Sep 1;127(Pt 17):3641-8
pubmed: 25128561
Nat Commun. 2020 Jul 17;11(1):3586
pubmed: 32681075
Nat Commun. 2017 Oct 27;8(1):1157
pubmed: 29079850
Cancer Res. 2006 Sep 1;66(17):8367-72
pubmed: 16951145
Mol Cell. 2006 Feb 3;21(3):337-47
pubmed: 16455489
PLoS One. 2015 Aug 12;10(8):e0135748
pubmed: 26267863
Curr Biol. 2012 Sep 11;22(17):1554-63
pubmed: 22795696
Proc Natl Acad Sci U S A. 2015 Dec 8;112(49):15160-5
pubmed: 26598692
Proteomics. 2017 Feb;17(3-4):
pubmed: 27723259
Cell. 2002 Sep 20;110(6):673-87
pubmed: 12297042
J Cell Biol. 1996 Dec;135(6 Pt 1):1633-42
pubmed: 8978828
J Cell Biol. 2020 Aug 3;219(8):
pubmed: 32585685
J Cell Sci. 2016 Nov 15;129(22):4159-4163
pubmed: 27799358
Cancer Res. 2012 May 15;72(10):2554-64
pubmed: 22589274
J Biol Chem. 2010 Nov 5;285(45):34875-84
pubmed: 20739287
J Biol Chem. 2018 Feb 2;293(5):1642-1650
pubmed: 29212708
Mol Biol Cell. 1994 Feb;5(2):203-15
pubmed: 8019006
Nat Cell Biol. 2007 Aug;9(8):858-67
pubmed: 17671451
PLoS One. 2014 Dec 19;9(12):e115213
pubmed: 25526367
Sci Signal. 2009 Sep 08;2(87):ra51
pubmed: 19738201
Mol Biol Cell. 2013 Aug;24(15):2454-66
pubmed: 23761073
Mol Cell Proteomics. 2014 Sep;13(9):2513-26
pubmed: 24942700
J Cell Biol. 2012 Mar 19;196(6):801-10
pubmed: 22412018
Cell Death Dis. 2018 Jul 23;9(8):802
pubmed: 30038287
Cancer Res. 2013 Mar 15;73(6):1969-80
pubmed: 23378338
Science. 2009 May 15;324(5929):895-9
pubmed: 19443776
Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2272-7
pubmed: 12606711
Cancer Res. 2009 Mar 15;69(6):2279-86
pubmed: 19276381
Biol Open. 2020 Aug 13;9(8):
pubmed: 32709696
Cancer Res. 2014 Nov 15;74(22):6671-81
pubmed: 25261239
J Cell Biol. 1999 Dec 13;147(6):1325-40
pubmed: 10601344
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
J Cell Biol. 2020 Feb 3;219(2):
pubmed: 31914171
Elife. 2016 Jul 13;5:
pubmed: 27410476
Nature. 2013 Jul 11;499(7457):166-71
pubmed: 23846654
Mol Cancer Res. 2004 Apr;2(4):225-32
pubmed: 15140944
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Science. 2013 Feb 15;339(6121):819-23
pubmed: 23287718
Nat Rev Mol Cell Biol. 2014 Apr;15(4):273-88
pubmed: 24651544
Proc Natl Acad Sci U S A. 2010 Jun 15;107(24):10920-5
pubmed: 20534451
Oncogene. 2011 Sep 29;30(39):4087-96
pubmed: 21478906
Cell. 1996 Nov 15;87(4):733-43
pubmed: 8929541
Biochemistry. 2014 Feb 11;53(5):810-20
pubmed: 24467163