Multiplex immunochromatographic platform based on crystal violet tag for simultaneous detection of streptomycin and chloramphenicol.
Chloramphenicol
Crystal violet
Multiplex immunochromatographic platform
Simultaneous detection
Streptomycin
Journal
Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639
Informations de publication
Date de publication:
01 Nov 2022
01 Nov 2022
Historique:
received:
13
11
2021
revised:
23
04
2022
accepted:
28
05
2022
pubmed:
12
6
2022
medline:
25
6
2022
entrez:
11
6
2022
Statut:
ppublish
Résumé
Antibiotic abuse has caused serious health risks for human beings for long. To address the problem, novel and facile detection techniques are highly desired. Here, an effective multiplex immunochromatographic platform (MICP) with synthesis-free and cost-effective merits is established for simultaneous detection of antibiotics on a single immunochromatographic assay (ICA) strip. Adopting crystal violet (CV) as a signal tag for multiplex ICA allows for direct coupling with multiple antibodies in several minutes. By combining CV and ICA perfectly, this convenient strategy offers improvements in convenience, speed, flexibility, and portability, eventually ensuring the optimized effectiveness of this approach. As a result, the established platform is successfully used to detect streptomycin (STR) and chloramphenicol (CAP) with visual detection mode, and the obtained total recoveries of milk and honey real samples changed from 83.82 to 113.38% with total RSD values of 0.48 to 4.15%.
Identifiants
pubmed: 35689929
pii: S0308-8146(22)01313-9
doi: 10.1016/j.foodchem.2022.133351
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Chloramphenicol
66974FR9Q1
Gentian Violet
J4Z741D6O5
Streptomycin
Y45QSO73OB
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
133351Informations de copyright
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