Expression and biological activity of lytic proteins HolST-3 and LysST-3 of Salmonella phage ST-3.

Animals Anti-Bacterial Agents / pharmacology Myoviridae Salmonella Salmonella Infections / therapy Salmonella Phages / genetics Zebrafish
Antibiotic resistance HolST-3 LysST-3 Protein expression Zebrafish

Journal

Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191

Informations de publication

Date de publication:
Aug 2022
Historique:
received: 31 03 2022
revised: 05 06 2022
accepted: 08 06 2022
pubmed: 14 6 2022
medline: 10 8 2022
entrez: 13 6 2022
Statut: ppublish

Résumé

Salmonella infection is a major public health concern. Several strategies for Salmonella infection prevention and control are currently available including vaccines and antibiotics. However, vaccines are expensive and inefficient, and the use of antibiotics can lead to antibiotic resistance. Thus, alternative strategies for the treatment of Salmonella remain warrant. In this study, recombinant holin HolST-3 and lysin LysST-3 from Salmonella phage ST-3 were expressed and purified, and their bactericidal properties were analyzed. HolST-3 and LysST-3 possessed a wider lysis spectrum and more efficient bactericidal effect than phage ST-3, and a synergistic bactericidal effect was observed when combined in vitro. In addition, we explored the bactericidal properties of HolST-3 and LysST-3 in vivo using zebrafish as a model organism, and found that the bactericidal effects of both HolST-3 and LysST-3 in vivo were comparable to those of cefotaxime, an antibiotic. This study provides a basis for the development of HolST-3 and LysST-3 as novel bactericidal agents for the prevention and treatment of infectious diseases caused by Salmonella spp.

Identifiants

DOI: 10.1016/j.micpath.2022.105624 PMID: 35697172
pubmed: 35697172
pii: S0882-4010(22)00237-6
doi: 10.1016/j.micpath.2022.105624
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

105624

Informations de copyright

Copyright © 2022 Elsevier Ltd. All rights reserved.

Auteurs

Bingxin Liu (B)

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Han Lu (H)

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Zong Li (Z)

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Peihan Yan (P)

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China.

Ruyin Liu (R)

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China. Electronic address: Lry1981@ucas.ac.cn.

Xinchun Liu (X)

College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, China. Electronic address: xcliu@ucas.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Expression and biological activity of lytic...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Expression and biological activity of lytic...
questionsmedicales.fr › Virus › Virus à ADN › Caudovirales › Myoviridae › Expression and biological activity of lytic...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Salmonella › Expression and biological activity of lytic...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Enterobacteriaceae › Salmonelloses › Expression and biological activity of lytic...
questionsmedicales.fr › Virus › Bactériophages › Phages de Salmonella › Expression and biological activity of lytic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Cypriniformes › Cyprinidae › Danio zébré › Expression and biological activity of lytic...