Single-dose ethanol intoxication causes acute and lasting neuronal changes in the brain.
Alcoholic Intoxication
/ metabolism
Animals
Behavior, Addictive
/ chemically induced
Dopaminergic Neurons
/ drug effects
Dose-Response Relationship, Drug
Drosophila melanogaster
Ethanol
/ administration & dosage
Gene Knockdown Techniques
Hippocampus
/ drug effects
Mice
Mitochondria
/ metabolism
Nerve Tissue Proteins
/ genetics
Protein Transport
/ drug effects
Drosophila
addiction
ethanol
plasticity
two-photon microscopy
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
21 06 2022
21 06 2022
Historique:
entrez:
14
6
2022
pubmed:
15
6
2022
medline:
18
6
2022
Statut:
ppublish
Résumé
Alcohol intoxication at early ages is a risk factor for the development of addictive behavior. To uncover neuronal molecular correlates of acute ethanol intoxication, we used stable-isotope-labeled mice combined with quantitative mass spectrometry to screen more than 2,000 hippocampal proteins, of which 72 changed synaptic abundance up to twofold after ethanol exposure. Among those were mitochondrial proteins and proteins important for neuronal morphology, including MAP6 and ankyrin-G. Based on these candidate proteins, we found acute and lasting molecular, cellular, and behavioral changes following a single intoxication in alcohol-naïve mice. Immunofluorescence analysis revealed a shortening of axon initial segments. Longitudinal two-photon in vivo imaging showed increased synaptic dynamics and mitochondrial trafficking in axons. Knockdown of mitochondrial trafficking in dopaminergic neurons abolished conditioned alcohol preference in
Identifiants
pubmed: 35700362
doi: 10.1073/pnas.2122477119
pmc: PMC9231489
doi:
Substances chimiques
Nerve Tissue Proteins
0
Ethanol
3K9958V90M
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2122477119Commentaires et corrections
Type : CommentIn
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