Microbial Interspecies Associations in Fracture-Related Infection.
Journal
Journal of orthopaedic trauma
ISSN: 1531-2291
Titre abrégé: J Orthop Trauma
Pays: United States
ID NLM: 8807705
Informations de publication
Date de publication:
01 06 2022
01 06 2022
Historique:
accepted:
16
11
2021
pubmed:
16
6
2022
medline:
18
6
2022
entrez:
15
6
2022
Statut:
ppublish
Résumé
Describe co-occurrence or clustering of microbial taxa in fracture-related infections to inform further exploration of infection-related interactions among them. Retrospective review. Level 1 trauma center. Four hundred twenty-three patients requiring surgical intervention for deep surgical site infection between January 2006 and December 2015. None. Connection between microbial taxa. Methicillin-resistant Staphylococcus aureus, methicillin-sensitive Staphylococcus aureus, and coagulase-negative Staphylococcus represented the majority of monomicrobial observations (71%). Gram-negative rods, gram-positive rods, and anaerobes presented more frequently in polymicrobial infections. Enterobacter, vancomycin-sensitive Enterococcus, and Pseudomonas are present in polymicrobial infections with the highest frequencies and represent the top 3 most important nodes within the microorganism framework, with the highest network centrality scores. The present study indicates that there are common microbial taxa (Enterobacter, Enterococcus, and Pseudomonas) that tend to co-occur with other microbes greater than 75% of the time. These commonly co-occurring microbes have demonstrated interactive relationships in other disease pathologies, suggesting that there may be similar important interactions in fracture-related infections. It is possible that these microbial communities play a role in the persistently high failure rate associated with management of infection after trauma. Future studies are needed to study the intermicrobial interactions that explain the frequency at which taxa co-occur. Understanding and potentially disrupting these intermicrobial relationships could inform improvements in the treatment of established infections and in the prevention of infection in high-risk patients. Prognostic Level IV. See Instructions for Authors for a complete description of levels of evidence.
Identifiants
pubmed: 35703847
doi: 10.1097/BOT.0000000000002314
pii: 00005131-202206000-00007
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
309-316Informations de copyright
Copyright © 2022 Wolters Kluwer Health, Inc. All rights reserved.
Déclaration de conflit d'intérêts
The authors report no conflict of interest.
Références
Bosse MJ, MacKenzie EJ, Kellam JF, et al. An analysis of outcomes of reconstruction or amputation after leg-threatening injuries. N Engl J Med. 2002;347:1924–1931.
Harris AM, Althausen PL, Kellam J, et al. Complications following limb-threatening lower extremity trauma. J Orthop Trauma. 2009;23:1–6.
Keeling JJ, Gwinn DE, Tintle SM, et al. Short-term outcomes of severe open wartime tibial fractures treated with ring external fixation. J Bone Joint Surg Am. 2008;90:2643–2651.
Lerner A, Fodor L, Soudry M. Is staged external fixation a valuable strategy for war injuries to the limbs? Clin Orthop. 2006;448:217–224.
Owens BD, Kragh JF Jr, Wenke JC, et al. Combat wounds in operation Iraqi Freedom and operation enduring freedom. J Trauma. 2008;64:295–299.
Merritt K. Factors increasing the risk of infection in patients with open fractures. J Trauma. 1988;28:823–827.
Dellinger EP, Miller SD, Wertz MJ, et al. Risk of infection after open fracture of the arm or leg. Arch Surg. 1988;123:1320–1327.
Yun HC, Murray CK, Nelson KJ, et al. Infection after orthopaedic trauma: prevention and treatment. J Orthop Trauma. 2016;30(suppl 3):S21–S26.
Ehrlichman L, Rackard F, Sparks M, et al. Factors associated with treatment failure of implant-related infections in fracture patients. Paper presented at Orthop Trauma Association Annual Meeting; 2018; Orlando FL.
Ovaska MT, Mäkinen TJ, Madanat R, et al. Risk factors for deep surgical site infection following operative treatment of ankle fractures. J Bone Joint Surg Am. 2013;95:348–353.
Rightmire E, Zurakowski D, Vrahas M. Acute infections after fracture repair: management with hardware in place. Clin Orthop. 2008;466:466–472.
Trampuz A, Zimmerli W. Diagnosis and treatment of infections associated with fracture-fixation devices. Injury. 2006;37(suppl 2):S59–S66.
Pollak AN, Calhoun JH. Extremity war injuries: state of the art and future directions. Prioritized future research objectives. J Am Acad Orthop Surg. 2006;14:S212–S214.
Murray CK, Obremskey WT, Hsu JR, et al. Prevention of infections associated with combat-related extremity injuries. J Trauma. 2011;71:S235–S257.
Gitajn IL, Titus AJ, Tosteson AN, et al. Deficits in preference-based health-related quality of life after complications associated with tibial fracture. Bone Joint J. 2018;100-B:1227–1233.
Høiby N, Bjarnsholt T, Givskov M, et al. Antibiotic resistance of bacterial biofilms. Int J Antimicrob Agents. 2010;35:322–332.
Hengzhuang W, Wu H, Ciofu O, et al. Pharmacokinetics/pharmacodynamics of colistin and imipenem on mucoid and nonmucoid pseudomonas aeruginosa biofilms. Antimicrob Agents Chemother. 2011;55:4469–4474.
Bjarnsholt T, Ciofu O, Molin S, et al. Applying insights from biofilm biology to drug development—can a new approach be developed? Nat Rev Drug Discov. 2013;12:791–808.
Burmolle M, Ren D, Bjarnsholt T, et al. Interactions in multispecies biofilms: do they matter? Trends Microbiol. 2014;22:84–91.
West SA, Diggle SP, Buckling A, et al. The social lives of microbes. Annu Rev Ecol Evol Syst. 2007;38:55–77.
Nadell CD, Xavier JB, Foster KR. The sociobiology of biofilms. FEMS Microbiol Rev. 2009;33:206–224.
Nadell CD, Foster KR. Mutually helping microbes can evolve by hitchhiking. Proc Natl Acad Sci U. S. A. 2012;109:19037–19038.
Schuster M, Lostroh CP, Ogi T, et al. Identification, timing, and signal specificity of Pseudomonas aeruginosa quorum-controlled genes: a transcriptome analysis. J Bacteriol. 2003;185:2066–2079.
O'Brien S, Fothergill JL. The role of multispecies social interactions in shaping Pseudomonas aeruginosa pathogenicity in the cystic fibrosis lung. FEMS Microbiol Lett. 2017;364:fnx128.
Montalvo RN, Natoli RM, O'Hara N, et al. Variations in the organisms causing deep surgical site infections in fracture patients at a level I trauma center (2006–2015). J Orthop Trauma. 2018;32:e475–e481.
Torbert JT, Joshi M, Moraff A, et al. Current bacterial speciation and antibiotic resistance in deep infections after operative fixation of fractures. J Orthop Trauma. 2015;29:7–17.
Mangram AJ, Horan TC, Pearson ML, et al.; Guideline for Prevention of Surgical Site Infection, 1999. Centers for disease control and prevention (CDC) hospital infection control practices advisory committee. Am J Infect Control. 1999;27:97–132; quiz 133–134; discussion 96 (1999).
Ibberson CB, Whiteley M. The social life of microbes in chronic infection. Curr Opin Microbiol. 2020;53:44–50.
Drescher K, Kunkel J, Nadell CD, et al. Architectural transitions in Vibrio cholerae biofilms at single-cell resolution. Proc Natl Acad Sci USA. 2016;14:113.
Nguyen AT, Oglesby-Sherrouse AG. Interactions between Pseudomonas aeruginosa and Staphylococcus aureus during co-cultivations and polymicrobial infections. Appl Microbiol Biotechnol. 2016;100:6141–6148.
Limoli DH, Yang J, Khansaheb MK, et al. Staphylococcus aureus and Pseudomonas aeruginosa co-infection is associated with cystic fibrosis-related diabetes and poor clinical outcomes. Eur J Clin Microbiol Infect Dis. 2016;35:947–953.
Gabrilska RA, Rumbaugh KP. Biofilm models of polymicrobial infection. Future Microbiol. 2015;10:1997–2015.
Limoli DH, Hoffman LR. Help, hinder, hide and harm: what can we learn from the interactions between Pseudomonas aeruginosa and Staphylococcus aureus during respiratory infections? Thorax. 2019;74:684–692.
Bergeron AC, Seman BG, Hammond JH, et al. Candida albicans and Pseudomonas aeruginosa interact to enhance virulence of mucosal infection in transparent cebrafish. Infect Immun. 2017;85:e00475–e00817.
Nogueira F, Sharghi S, Kuchler K, et al. Pathogenetic impact of bacterial-fungal interactions. Microorganisms. 2019;7:459.
Shahidi A, Ellner PD. Effect of mixed cultures on antibiotic susceptibility testing. Appl Microbiol. 1969;18:766–770.
Hotterbeekx A, Kumar-Singh S, Goossens H, et al. In vivo and in vitro interactions between Pseudomonas aeruginosa and Staphylococcus spp. Front Cell Infect Microbiol. 2017;7:106.
Peleg AY, Hogan DA, Mylonakis E. Medically important bacterial-fungal interactions. Nat Rev Microbiol. 2010;8:340–349.
Orazi G, O'Toole GA. Pseudomonas aeruginosa alters Staphylococcus aureus sensitivity to vancomycin in a biofilm model of cystic fibrosis infection. mBio. 2017;8:e00873–e00917.
Cavalcanti IM, Del Bel Cury AA, Jenkinson HF, et al. Interactions between Streptococcus oralis, Actinomyces oris, and Candida albicans in the development of multispecies oral microbial biofilms on salivary pellicle. Mol Oral Microbiol. 2017;32:60–73.
Bartlett JG, Onderdonk AB, Louie T, et al. A review. Lessons from an animal model of intraabdominal sepsis. Arch Surg. 1978;113:853–857.
Ding J, Zhang Y, Deng Y, et al. Integrated metagenomics and network analysis of soil microbial community of the forest timberline. Sci Rep. 2015;5:7994.
Knutas A, Hajikhani A, Salminen J, et al. Cloud-Based Bibliometric Analysis Service for Systematic Mapping Studies. Proceedings of the 16th International Conference on Computer Systems and Technologies (CompSysTech '15). Association for Computing Machinery, New York, NY; 184–191. Available at: https://doi-org.dartmouth.idm.oclc.org/10.1145/2812428.2812442 .
Labus JS, Osadchiy V, Hsiao EY, et al. Evidence for an association of gut microbial clostridia with brain functional connectivity and gastrointestinal sensorimotor function in patients with irritable bowel syndrome, based on tripartite network analysis. Microbiome. 2019;7:45.
Lawson C, Wu S, Bhattacharjee A, et al. Metabolic network analysis reveals microbial community interactions in anammox granules. Nat Commun. 2017;8:15416.