Genome-wide identification of fitness determinants in the Xanthomonas campestris bacterial pathogen during early stages of plant infection.
Brassica oleracea
Xanthomonas
RB-TnSeq
fitness
hydathodes
xylem
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
28
03
2022
accepted:
07
06
2022
pubmed:
17
6
2022
medline:
11
9
2022
entrez:
16
6
2022
Statut:
ppublish
Résumé
Plant diseases are an important threat to food production. While major pathogenicity determinants required for disease have been extensively studied, less is known on how pathogens thrive during host colonization, especially at early infection stages. Here, we used randomly barcoded-transposon insertion site sequencing (RB-TnSeq) to perform a genome-wide screen and identify key bacterial fitness determinants of the vascular pathogen Xanthomonas campestris pv campestris (Xcc) during infection of the cauliflower host plant (Brassica oleracea). This high-throughput analysis was conducted in hydathodes, the natural entry site of Xcc, in xylem sap and in synthetic media. Xcc did not face a strong bottleneck during hydathode infection. In total, 181 genes important for fitness were identified in plant-associated environments with functional enrichment in genes involved in metabolism but only few genes previously known to be involved in virulence. The biological relevance of 12 genes was independently confirmed by phenotyping single mutants. Notably, we show that XC_3388, a protein with no known function (DUF1631), plays a key role in the adaptation and virulence of Xcc possibly through c-di-GMP-mediated regulation. This study revealed yet unsuspected social behaviors adopted by Xcc individuals when confined inside hydathodes at early infection stages.
Identifiants
pubmed: 35706385
doi: 10.1111/nph.18313
pmc: PMC9543026
doi:
Substances chimiques
Bacterial Proteins
0
Banques de données
RefSeq
['SRP363574', 'SRP363580']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
235-248Informations de copyright
© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation.
Références
mSystems. 2022 Apr 26;7(2):e0129021
pubmed: 35311560
Mol Plant Pathol. 2019 Feb;20(2):287-306
pubmed: 30267562
New Phytol. 2019 Apr;222(1):455-467
pubmed: 30447163
Proc Natl Acad Sci U S A. 2019 Sep 17;116(38):18900-18910
pubmed: 31484768
Nat Rev Microbiol. 2015 Aug;13(8):497-508
pubmed: 26145732
New Phytol. 2022 Jan;233(2):905-918
pubmed: 34655498
Genome Res. 2005 Jun;15(6):757-67
pubmed: 15899963
Nat Rev Genet. 2020 Sep;21(9):526-540
pubmed: 32533119
Proc Natl Acad Sci U S A. 1987 May;84(9):2833-7
pubmed: 2883655
Gene. 1994 Jul 22;145(1):69-73
pubmed: 8045426
Annu Rev Phytopathol. 2020 Aug 25;58:55-75
pubmed: 32600178
mBio. 2012 May 02;3(2):
pubmed: 22448042
FEMS Microbiol Rev. 2020 Jan 1;44(1):1-32
pubmed: 31578554
Mol Plant Pathol. 2018 Feb 26;:
pubmed: 29480976
mBio. 2015 May 12;6(3):e00306-15
pubmed: 25968644
Mol Plant Pathol. 2007 Jul;8(4):437-50
pubmed: 20507512
J Bacteriol. 2007 Mar;189(5):2055-62
pubmed: 17158655
PLoS Pathog. 2016 Nov 30;12(11):e1006019
pubmed: 27902780
Science. 2021 Aug 20;373(6557):871-876
pubmed: 34282049
PLoS One. 2007 Feb 21;2(2):e224
pubmed: 17311090
Mol Cells. 2019 Feb 28;42(2):166-174
pubmed: 30759970
Bioinformatics. 2006 Jul 1;22(13):1600-7
pubmed: 16606683
Mol Microbiol. 2009 Mar;71(6):1464-76
pubmed: 19220743
Plant Physiol. 2017 Jun;174(2):700-716
pubmed: 28184011
Mol Microbiol. 2003 Aug;49(4):991-1003
pubmed: 12890023
Annu Rev Genet. 2020 Nov 23;54:337-365
pubmed: 32886545
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51
pubmed: 7012838
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1648-52
pubmed: 377280
Microbiol Spectr. 2021 Dec 22;9(3):e0205721
pubmed: 34935430
Mol Plant Pathol. 2022 Feb;23(2):159-174
pubmed: 34837293
Mol Ecol. 2017 Nov;26(21):5939-5952
pubmed: 28869687
mSystems. 2020 Mar 31;5(2):
pubmed: 32234775
FEMS Microbiol Lett. 2010 Mar;304(1):20-8
pubmed: 20082640
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Mol Microbiol. 2007 Jan;63(2):429-42
pubmed: 17241199
Nat Rev Microbiol. 2012 Dec;10(12):828-40
pubmed: 23154261
Bio Protoc. 2022 Jul 05;12(13):e3776
pubmed: 35991161
Trends Microbiol. 2021 Feb;29(2):182-183
pubmed: 32616307
Nature. 2018 May;557(7706):503-509
pubmed: 29769716
Environ Microbiol. 2016 Oct;18(10):3593-3605
pubmed: 27516206
Mol Plant Pathol. 2018 Mar;19(3):537-551
pubmed: 28120374
Nature. 2013 Feb 21;494(7437):353-6
pubmed: 23426324
PLoS Pathog. 2011 Jul;7(7):e1002143
pubmed: 21829349
Annu Rev Phytopathol. 2019 Aug 25;57:91-116
pubmed: 31100996
mSystems. 2021 Oct 26;6(5):e0083821
pubmed: 34636662
PLoS One. 2020 Sep 28;15(9):e0239998
pubmed: 32986776
Environ Microbiol. 2019 Aug;21(8):3140-3152
pubmed: 31209989
Mol Plant Microbe Interact. 2008 Feb;21(2):151-61
pubmed: 18184059
Mol Plant Pathol. 2013 Jan;14(1):2-18
pubmed: 23051837
FEMS Microbiol Ecol. 2014 Sep;89(3):527-41
pubmed: 24784488
Nat Rev Microbiol. 2020 Aug;18(8):415-427
pubmed: 32346148
Genome Biol. 2007;8(10):R218
pubmed: 17927820
FEMS Microbiol Rev. 2010 Mar;34(2):107-33
pubmed: 19925633
Nat Rev Microbiol. 2021 Apr;19(4):256-271
pubmed: 33149273
Mol Microbiol. 2007 Apr;64(2):281-92
pubmed: 17378922
Mol Plant Microbe Interact. 1991 Nov-Dec;4(6):593-601
pubmed: 1666525