Structures of an active type III-A CRISPR effector complex.
CRISPR
complex with ATP
complex with target RNA
crRNA
cryo-EM
structure
type III-A
Journal
Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697
Informations de publication
Date de publication:
04 08 2022
04 08 2022
Historique:
received:
30
04
2022
revised:
09
05
2022
accepted:
17
05
2022
pubmed:
18
6
2022
medline:
10
8
2022
entrez:
17
6
2022
Statut:
ppublish
Résumé
Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) provide many prokaryotes with an adaptive immune system against invading genetic material. Type III CRISPR systems are unique in that they can degrade both RNA and DNA. In response to invading nucleic acids, they produce cyclic oligoadenylates that act as secondary messengers, activating cellular nucleases that aid in the immune response. Here, we present seven single-particle cryo-EM structures of the type III-A Staphylococcus epidermidis CRISPR effector complex. The structures reveal the intact S. epidermidis effector complex in an apo, ATP-bound, cognate target RNA-bound, and non-cognate target RNA-bound states and illustrate how the effector complex binds and presents crRNA. The complexes bound to target RNA capture the type III-A effector complex in a post-RNA cleavage state. The ATP-bound structures give details about how ATP binds to Cas10 to facilitate cyclic oligoadenylate production.
Identifiants
pubmed: 35714601
pii: S0969-2126(22)00187-3
doi: 10.1016/j.str.2022.05.013
pmc: PMC9357104
mid: NIHMS1811052
pii:
doi:
Substances chimiques
CRISPR-Associated Proteins
0
RNA
63231-63-0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1109-1128.e6Subventions
Organisme : NIGMS NIH HHS
ID : U24 GM129541
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118108
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR001422
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA060553
Pays : United States
Organisme : NIGMS NIH HHS
ID : U24 GM129539
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
Nature. 2015 Oct 1;526(7571):55-61
pubmed: 26432244
Microbiology (Reading). 2005 Mar;151(Pt 3):653-663
pubmed: 15758212
Science. 2017 Aug 11;357(6351):605-609
pubmed: 28663439
Mol Cell. 2019 Jan 17;73(2):264-277.e5
pubmed: 30503773
Nucleic Acids Res. 2019 Apr 23;47(7):3765-3783
pubmed: 30759237
Nat Rev Microbiol. 2020 Feb;18(2):67-83
pubmed: 31857715
Nat Commun. 2020 Jan 24;11(1):500
pubmed: 31980625
PLoS Comput Biol. 2005 Nov;1(6):e60
pubmed: 16292354
RNA Biol. 2013 Nov;10(11):1670-8
pubmed: 24157656
Cell Host Microbe. 2017 Sep 13;22(3):343-353.e3
pubmed: 28826839
J Biol Chem. 2013 Sep 27;288(39):27888-97
pubmed: 23935102
Nat Methods. 2017 Aug;14(8):793-796
pubmed: 28671674
Mol Cell. 2012 Feb 10;45(3):303-13
pubmed: 22227115
Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21218-22
pubmed: 22160698
Mol Microbiol. 2002 Mar;43(6):1565-75
pubmed: 11952905
Mol Cell. 2013 Oct 10;52(1):124-34
pubmed: 24119402
Mol Cell. 2014 Nov 20;56(4):506-17
pubmed: 25458845
Microbiology (Reading). 2005 Aug;151(Pt 8):2551-2561
pubmed: 16079334
Nucleic Acids Res. 2017 Feb 28;45(4):2112-2123
pubmed: 28204542
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Cell. 2019 Jan 10;176(1-2):239-253.e16
pubmed: 30503210
Mol Cell. 2010 Jan 15;37(1):7-19
pubmed: 20129051
Trends Microbiol. 2017 Jan;25(1):49-61
pubmed: 27773522
Cell Res. 2018 Dec;28(12):1195-1197
pubmed: 30459428
J Struct Biol. 2015 Nov;192(2):216-21
pubmed: 26278980
Nat Methods. 2017 Mar;14(3):290-296
pubmed: 28165473
BMC Bioinformatics. 2007 May 23;8:172
pubmed: 17521438
Science. 2021 Aug 20;373(6557):871-876
pubmed: 34282049
Nucleic Acids Res. 2014 Jan;42(2):1129-38
pubmed: 24121684
Nat Commun. 2020 Mar 27;11(1):1596
pubmed: 32221291
Nature. 2020 Jan;577(7791):572-575
pubmed: 31942067
Science. 2015 May 1;348(6234):581-5
pubmed: 25837515
Nat Rev Genet. 2010 Mar;11(3):181-90
pubmed: 20125085
Int J Biol Macromol. 2021 Feb 15;170:140-149
pubmed: 33352158
Mol Cell. 2016 Apr 21;62(2):295-306
pubmed: 27105119
Nat Rev Microbiol. 2014 Jul;12(7):479-92
pubmed: 24909109
Science. 2008 Dec 19;322(5909):1843-5
pubmed: 19095942
Acta Crystallogr D Struct Biol. 2018 Jun 1;74(Pt 6):531-544
pubmed: 29872004
J Mol Biol. 2003 Oct 31;333(4):721-45
pubmed: 14568533
Nat Commun. 2019 Sep 20;10(1):4302
pubmed: 31541109
Biochem Biophys Res Commun. 2020 Feb 26;523(1):112-116
pubmed: 31836139
Science. 2007 Mar 23;315(5819):1709-12
pubmed: 17379808
Commun Biol. 2021 Jul 15;4(1):874
pubmed: 34267316
Nature. 2021 Feb;590(7847):624-629
pubmed: 33461211
Protein Sci. 2021 Jan;30(1):70-82
pubmed: 32881101
Elife. 2019 Apr 03;8:
pubmed: 30942690
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Nat Rev Microbiol. 2019 Jan;17(1):7-12
pubmed: 30171202
J Struct Biol. 2016 Aug;195(2):190-198
pubmed: 27288865
Mol Cell. 2014 Nov 20;56(4):518-30
pubmed: 25457165
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
J Bacteriol. 2014 Jan;196(2):310-7
pubmed: 24187086
Nature. 2017 Aug 31;548(7669):543-548
pubmed: 28722012
Nat Rev Microbiol. 2015 Nov;13(11):722-36
pubmed: 26411297
Nat Commun. 2019 Jul 5;10(1):3001
pubmed: 31278272
Nature. 2010 Jan 28;463(7280):568-71
pubmed: 20072129
Science. 2020 Jul 3;369(6499):54-59
pubmed: 32467331
Nat Methods. 2017 Apr;14(4):331-332
pubmed: 28250466
Cell. 2009 Nov 25;139(5):945-56
pubmed: 19945378
Cell Res. 2019 Apr;29(4):305-312
pubmed: 30814678
J Mol Biol. 2015 Jan 30;427(2):259-73
pubmed: 25451598
Cell. 2015 May 21;161(5):1164-1174
pubmed: 25959775
Nucleic Acids Res. 2015 Jan;43(1):406-17
pubmed: 25505143