EFR3 and phosphatidylinositol 4-kinase IIIα regulate insulin-stimulated glucose transport and GLUT4 dispersal in 3T3-L1 adipocytes.
GLUT4
adipocytes
glucose transport
insulin
Journal
Bioscience reports
ISSN: 1573-4935
Titre abrégé: Biosci Rep
Pays: England
ID NLM: 8102797
Informations de publication
Date de publication:
29 07 2022
29 07 2022
Historique:
received:
08
06
2022
revised:
21
06
2022
accepted:
22
06
2022
pubmed:
24
6
2022
medline:
12
7
2022
entrez:
23
6
2022
Statut:
ppublish
Résumé
Insulin stimulates glucose transport in muscle and adipocytes. This is achieved by regulated delivery of intracellular glucose transporter (GLUT4)-containing vesicles to the plasma membrane where they dock and fuse, resulting in increased cell surface GLUT4 levels. Recent work identified a potential further regulatory step, in which insulin increases the dispersal of GLUT4 in the plasma membrane away from the sites of vesicle fusion. EFR3 is a scaffold protein that facilitates localization of phosphatidylinositol 4-kinase type IIIα to the cell surface. Here we show that knockdown of EFR3 or phosphatidylinositol 4-kinase type IIIα impairs insulin-stimulated glucose transport in adipocytes. Using direct stochastic reconstruction microscopy, we also show that EFR3 knockdown impairs insulin stimulated GLUT4 dispersal in the plasma membrane. We propose that EFR3 plays a previously unidentified role in controlling insulin-stimulated glucose transport by facilitating dispersal of GLUT4 within the plasma membrane.
Identifiants
pubmed: 35735144
pii: 231469
doi: 10.1042/BSR20221181
pmc: PMC9272592
pii:
doi:
Substances chimiques
Glucose Transport Proteins, Facilitative
0
Glucose Transporter Type 4
0
Insulin
0
1-Phosphatidylinositol 4-Kinase
EC 2.7.1.67
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : British Heart Foundation
ID : PG/18/47/33822
Pays : United Kingdom
Informations de copyright
© 2022 The Author(s).
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