The stimulatory effect of fusobacteria on dendritic cells under aerobic or anaerobic conditions.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
23 06 2022
23 06 2022
Historique:
received:
02
08
2021
accepted:
15
06
2022
entrez:
23
6
2022
pubmed:
24
6
2022
medline:
28
6
2022
Statut:
epublish
Résumé
Fusobacteria have been suspected to be pathobionts of colon cancer and inflammatory bowel disease. However, the immunomodulatory properties that affect these inflammatory reactions in dendritic cells (DCs) under anaerobic and aerobic conditions have not yet been characterized. We directly assessed the stimulatory effects of anaerobic commensal bacteria, including fusobacteria, on a human DC line through coculture under aerobic or anaerobic conditions. Under aerobic or anaerobic conditions, stimulation of the DC line with all live commensal bacteria examined, except the probiotic Lactobacillus delbrueckii subsp. bulgaricus (L. bulgaricus), significantly increased the geometric mean fluorescent intensity (MFI) of marker proteins (HLA-ABC, HLA-DR, CD80, CD86, CD83, or CCR7) on the DC surface. In particular, both Fusobacterium nucleatum (F. nucleatum) and Escherichia coli (E. coli) significantly increased the expression of DC-associated molecules, except for CD83 under both aerobic and anaerobic conditions. The DC line stimulated with Fusobacterium varium (F. varium) significantly increased only CD80, HLA-ABC, and HLA-DR expression under anaerobic conditions. Moreover, differences in the levels of proinflammatory cytokines, such as IL-6, IL-8, and TNF-α, were detected in the DC line stimulated by all live commensal bacteria under either aerobic or anaerobic conditions. Under aerobic conditions, the DC line stimulated with E. coli produced significantly more IL-6, IL-8, and TNF-α than did the cells stimulated with any of the bacteria examined. When E. coli were used to stimulate the DC line under anaerobic conditions, TNF-α was predominantly produced compared to stimulation with any other bacteria. Compared to the DC line stimulated with any other bacteria, the cells stimulated with F. nucleatum showed significantly increased production of IL-6, IL-8 and TNF-α only under anaerobic conditions. In particular, E. coli, F. nucleatum, and F. varium strongly stimulated the DC line, resulting in significantly increased expression of surface molecules associated with DCs and production of inflammatory cytokines.
Identifiants
pubmed: 35739324
doi: 10.1038/s41598-022-14934-z
pii: 10.1038/s41598-022-14934-z
pmc: PMC9225986
doi:
Substances chimiques
B7-1 Antigen
0
Cytokines
0
HLA-DR Antigens
0
Interleukin-6
0
Interleukin-8
0
Tumor Necrosis Factor-alpha
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10698Informations de copyright
© 2022. The Author(s).
Références
Leuk Res. 2009 Sep;33(9):1224-32
pubmed: 19443030
Blood. 2008 Nov 1;112(9):3713-22
pubmed: 18698004
Genome Res. 2012 Feb;22(2):299-306
pubmed: 22009989
Nat Rev Immunol. 2008 Jun;8(6):411-20
pubmed: 18469830
Curr Mol Med. 2003 Jun;3(4):373-85
pubmed: 12776992
Biol Cell. 2019 Sep;111(9):232-244
pubmed: 31187884
Gut. 2020 Nov;69(11):1998-2007
pubmed: 32051205
J Immunol. 2002 Jan 1;168(1):171-8
pubmed: 11751960
World J Gastroenterol. 2014 Oct 21;20(39):14420-9
pubmed: 25339828
Annu Rev Med. 2000;51:289-98
pubmed: 10774465
Curr Drug Targets. 2008 May;9(5):395-403
pubmed: 18473768
Leuk Res. 2010 Nov;34(11):1517-24
pubmed: 20338639
DNA Cell Biol. 2012 Aug;31(8):1431-8
pubmed: 22741617
J Gastroenterol Hepatol. 2002 Aug;17(8):849-53
pubmed: 12164960
Curr Opin Immunol. 2008 Dec;20(6):669-75
pubmed: 18852045
Cell Rep. 2013 Jun 27;3(6):1932-44
pubmed: 23727238
Mol Immunol. 2010 May;47(9):1765-73
pubmed: 20347150
Annu Rev Immunol. 1991;9:271-96
pubmed: 1910679
Adv Clin Exp Med. 2017 May-Jun;26(3):421-426
pubmed: 28791816
PLoS One. 2019 Feb 22;14(2):e0212406
pubmed: 30794590
Immunol Lett. 2013 Feb;150(1-2):30-40
pubmed: 23352670
PLoS One. 2014 Jan 29;9(1):e86702
pubmed: 24489770
Science. 2017 Dec 15;358(6369):1443-1448
pubmed: 29170280
Oncogene. 2015 Jul;34(27):3493-503
pubmed: 25174402
Int J Oncol. 2014 Jul;45(1):470-8
pubmed: 24819411
PLoS One. 2013;8(3):e59280
pubmed: 23555011
J Biol Chem. 2001 Jul 13;276(28):25680-6
pubmed: 11316801
Scand J Gastroenterol. 2005 Nov;40(11):1334-42
pubmed: 16334443
Front Immunol. 2017 Apr 18;8:427
pubmed: 28458670
Nat Rev Microbiol. 2014 Oct;12(10):661-72
pubmed: 25198138
Gut. 2003 Jan;52(1):79-83
pubmed: 12477765
Cell Host Microbe. 2013 Aug 14;14(2):207-15
pubmed: 23954159
Inflamm Bowel Dis. 2011 Sep;17(9):1971-8
pubmed: 21830275
Eur J Immunol. 2010 Jul;40(7):1830-5
pubmed: 20583029
Cell. 2006 Feb 24;124(4):837-48
pubmed: 16497592
Mol Immunol. 2002 Dec;39(9):531-6
pubmed: 12431386
Cell Immunol. 2011;267(1):17-22
pubmed: 21109237
Cell. 2017 Feb 23;168(5):928-943.e11
pubmed: 28215708
Nat Rev Immunol. 2009 May;9(5):313-23
pubmed: 19343057
Front Bioeng Biotechnol. 2019 Feb 07;7:13
pubmed: 30792981
Gastroenterology. 2011 May;140(6):1756-1767
pubmed: 21530742
J Med Microbiol. 2009 May;58(Pt 5):535-545
pubmed: 19369513
Nat Immunol. 2000 Sep;1(3):199-205
pubmed: 10973276
Cytokine Growth Factor Rev. 2008 Feb;19(1):41-52
pubmed: 18258476