Ellagic acid effects on testis, sex hormones, oxidative stress, and apoptosis in the relative sterility rat model following busulfan administration.
Animals
Antioxidants
/ pharmacology
Apoptosis
Busulfan
/ metabolism
Caspase 9
/ metabolism
Ellagic Acid
/ metabolism
Follicle Stimulating Hormone
/ metabolism
Infertility
/ metabolism
Male
Oxidative Stress
Proto-Oncogene Proteins c-bcl-2
/ metabolism
Rats
Spermatozoa
Testis
Testosterone
/ metabolism
Ellagic acid
Rat
Sex hormones
Sterility
Testicular tissue
Journal
BMC complementary medicine and therapies
ISSN: 2662-7671
Titre abrégé: BMC Complement Med Ther
Pays: England
ID NLM: 101761232
Informations de publication
Date de publication:
23 Jun 2022
23 Jun 2022
Historique:
received:
15
03
2022
accepted:
14
06
2022
entrez:
23
6
2022
pubmed:
24
6
2022
medline:
28
6
2022
Statut:
epublish
Résumé
Busulfan is an antineoplastic medication that is broadly utilized for cancer treatment. It affects the testicular function and leads to sterility. The present study aimed to evaluate the effects of ellagic acid on testicular tissue changes, sexual hormones, antioxidant defense system, and caspase-9 and Bcl2 gene expression in the busulfan-induced relative sterile rat model. This is an interventional-experimental animal study that was performed on 65 Adult male rats; they were randomly divided into five groups including control (1 ml of 0.9% normal saline), ellagic acid (50 mg/kg); busulfan (10 mg/kg); and busulfan plus ellagic acid (10 mg/kg and 50 mg/kg). At the end of the experiment, blood samples were collected, and plasma levels of sex hormones, antioxidant system, apoptosis-related genes, and testis histology were assessed. Busulfan reduced the levels of serum testosterone, total antioxidant capacity, gene expression of Bcl2, testicular volume, seminiferous tubule, germinal epithelium, interstitial tissue volume, and the number of spermatogonia, spermatocyte, round spermatid, elongated spermatid, Sertoli cells and Leydig cells (p < 0.05). Busulfan administration resulted in a significant increase (p < 0.05) in the level of LH, FSH, malondialdehyde, and caspase 9. Busulfan + ellagic acid (50 mg/kg) showed higher serum levels of testosterone, gene expression of Bcl-2 and antioxidant markers, and lower LH, FSH levels, and gene expression of caspase 9 compared to the Busulfan-treated rats (p < 0.05). Stereological parameters were also ameliorated in the group treated with Busulfan+ 50 mg/kg ellagic acid (p < 0.05). In conclusion, the consumption of ellagic acid may have beneficial effects on the antioxidant defense system, sexual hormone abnormality, and testicular tissue damage induced by busulfan.
Sections du résumé
BACKGROUND
BACKGROUND
Busulfan is an antineoplastic medication that is broadly utilized for cancer treatment. It affects the testicular function and leads to sterility. The present study aimed to evaluate the effects of ellagic acid on testicular tissue changes, sexual hormones, antioxidant defense system, and caspase-9 and Bcl2 gene expression in the busulfan-induced relative sterile rat model.
METHODS
METHODS
This is an interventional-experimental animal study that was performed on 65 Adult male rats; they were randomly divided into five groups including control (1 ml of 0.9% normal saline), ellagic acid (50 mg/kg); busulfan (10 mg/kg); and busulfan plus ellagic acid (10 mg/kg and 50 mg/kg). At the end of the experiment, blood samples were collected, and plasma levels of sex hormones, antioxidant system, apoptosis-related genes, and testis histology were assessed.
RESULTS
RESULTS
Busulfan reduced the levels of serum testosterone, total antioxidant capacity, gene expression of Bcl2, testicular volume, seminiferous tubule, germinal epithelium, interstitial tissue volume, and the number of spermatogonia, spermatocyte, round spermatid, elongated spermatid, Sertoli cells and Leydig cells (p < 0.05). Busulfan administration resulted in a significant increase (p < 0.05) in the level of LH, FSH, malondialdehyde, and caspase 9. Busulfan + ellagic acid (50 mg/kg) showed higher serum levels of testosterone, gene expression of Bcl-2 and antioxidant markers, and lower LH, FSH levels, and gene expression of caspase 9 compared to the Busulfan-treated rats (p < 0.05). Stereological parameters were also ameliorated in the group treated with Busulfan+ 50 mg/kg ellagic acid (p < 0.05).
CONCLUSION
CONCLUSIONS
In conclusion, the consumption of ellagic acid may have beneficial effects on the antioxidant defense system, sexual hormone abnormality, and testicular tissue damage induced by busulfan.
Identifiants
pubmed: 35739528
doi: 10.1186/s12906-022-03650-w
pii: 10.1186/s12906-022-03650-w
pmc: PMC9229441
doi:
Substances chimiques
Antioxidants
0
Proto-Oncogene Proteins c-bcl-2
0
Ellagic Acid
19YRN3ZS9P
Testosterone
3XMK78S47O
Follicle Stimulating Hormone
9002-68-0
Caspase 9
EC 3.4.22.-
Busulfan
G1LN9045DK
Types de publication
Journal Article
Randomized Controlled Trial, Veterinary
Langues
eng
Sous-ensembles de citation
IM
Pagination
170Informations de copyright
© 2022. The Author(s).
Références
Mol Reprod Dev. 2019 Jun;86(6):738-748
pubmed: 31041823
Andrologia. 2020 Dec;52(11):e13826
pubmed: 32991040
Free Radic Res. 2002 Nov;36(11):1209-18
pubmed: 12592673
Am J Clin Nutr. 2005 Jan;81(1 Suppl):277S-283S
pubmed: 15640491
Nitric Oxide. 2015 Jan 30;44:24-30
pubmed: 25461274
Mol Cell. 2000 Dec;6(6):1389-99
pubmed: 11163212
Cardiovasc Res. 2008 Oct 1;80(1):69-77
pubmed: 18567640
Syst Biol Reprod Med. 2018 Oct;64(5):348-357
pubmed: 29557179
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Feb 15;173:910-917
pubmed: 27829207
Am J Kidney Dis. 2006 Feb;47(2):263-76
pubmed: 16431255
BMC Endocr Disord. 2020 Oct 29;20(1):162
pubmed: 33121469
Ultrastruct Pathol. 2019;43(4-5):170-183
pubmed: 31658851
Indian J Physiol Pharmacol. 2014 Oct-Dec;58(4):416-22
pubmed: 26215011
Exp Cell Res. 2000 Apr 10;256(1):27-33
pubmed: 10739648
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11303-7
pubmed: 7972054
Asian J Androl. 2010 Mar;12(2):263-70
pubmed: 20010847
Iran J Reprod Med. 2013 Sep;11(9):693-704
pubmed: 24639808
Andrologia. 1995 Jul-Aug;27(4):223-8
pubmed: 7486033
Nat Rev Mol Cell Biol. 2014 Jan;15(1):49-63
pubmed: 24355989
J Carcinog. 2007 Dec 03;6:19
pubmed: 18053169
Probiotics Antimicrob Proteins. 2019 Dec;11(4):1172-1181
pubmed: 30406893
Clin Chim Acta. 2004 Jan;339(1-2):1-9
pubmed: 14687888
Pharm Biol. 2016 Oct;54(10):1947-53
pubmed: 26828763
Annu Rev Cell Dev Biol. 1999;15:269-90
pubmed: 10611963
FEBS Lett. 2004 Sep 24;575(1-3):41-51
pubmed: 15388331
Biosci Rep. 2017 Dec 12;37(6):
pubmed: 29101242
Sci Rep. 2018 Jan 23;8(1):1465
pubmed: 29362375
Cytotechnology. 2018 Apr;70(2):497-502
pubmed: 29350306
Proc Natl Acad Sci U S A. 1988 Apr;85(7):2329-33
pubmed: 2965391
J Carcinog. 2011;10:27
pubmed: 22190869
Vet World. 2019 Aug;12(8):1261-1265
pubmed: 31641306
J Nutr Biochem. 2005 Nov;16(11):675-81
pubmed: 16081267
Asian J Androl. 2002 Sep;4(3):201-7
pubmed: 12364977
Anticancer Res. 2006 Sep-Oct;26(5A):3601-6
pubmed: 17094489
Circ Res. 2001 Apr 13;88(7):733-9
pubmed: 11304497
J Nutr Biochem. 2005 Jun;16(6):360-7
pubmed: 15936648
J Androl. 2005 Nov-Dec;26(6):698-705
pubmed: 16291964
Arch Dis Child. 1991 Apr;66(4):517-20
pubmed: 1903244
Hum Exp Toxicol. 2020 Aug;39(8):1019-1030
pubmed: 32153207
Andrology. 2020 Jan;8(1):249-258
pubmed: 31325243
J Cell Physiol. 2021 May;236(5):3495-3509
pubmed: 33030247
J Carcinog. 2007 Apr 18;6:4
pubmed: 17442116
Biomed Pharmacother. 2018 Jul;103:1464-1472
pubmed: 29864931
Can J Physiol Pharmacol. 1985 Sep;63(9):1155-8
pubmed: 4052873
J Carcinog. 2010 Jul 14;9:
pubmed: 20808823
Nat Rev Mol Cell Biol. 2004 Sep;5(9):752-62
pubmed: 15340382
Fertil Steril. 2008 May;89(5 Suppl):1474-81
pubmed: 17681317
Int J Fertil Steril. 2019 Apr;13(1):57-65
pubmed: 30644246
J Nat Sci Biol Med. 2013 Jul;4(2):414-9
pubmed: 24082743
Ann Oncol. 2004;15 Suppl 4:iv259-65
pubmed: 15477318
BMC Nephrol. 2021 May 2;22(1):162
pubmed: 33933022
Biol Reprod. 1985 Dec;33(5):1213-22
pubmed: 3000465
Neurol Sci. 2012 Jun;33(3):567-74
pubmed: 21922312
Exp Toxicol Pathol. 2007 Oct;59(2):97-103
pubmed: 17716881
Andrologia. 2022 May;54(4):e14375
pubmed: 35266181
Iran J Basic Med Sci. 2015 Jan;18(1):38-46
pubmed: 25810874
Drug Chem Toxicol. 2022 Jan;45(1):22-32
pubmed: 31469002
Reprod Toxicol. 2001 Nov-Dec;15(6):611-7
pubmed: 11738514
Cell J. 2019 Apr;21(1):7-13
pubmed: 30507083
J Natl Cancer Inst Monogr. 2005;(34):12-7
pubmed: 15784814
Ann Anat. 2014 Jan;196(1):57-66
pubmed: 23069191
Biochem Pharmacol. 1992 Aug 18;44(4):747-53
pubmed: 1510722
Bone Marrow Transplant. 2000 May;25(10):1087-92
pubmed: 10828870
Endocrinol Exp. 1990 Mar;24(1-2):267-73
pubmed: 2113858
Clin Exp Reprod Med. 2013 Jun;40(2):60-6
pubmed: 23875161
Andrologia. 2019 Mar;51(2):e13193
pubmed: 30478946