Zebrafish fin regeneration involves generic and regeneration-specific osteoblast injury responses.
actomyosin
bone regeneration
cell biology
cell migration
complement system
dedifferentiation
developmental biology
osteoblast
zebrafish
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
24 06 2022
24 06 2022
Historique:
received:
04
02
2022
accepted:
23
06
2022
pubmed:
25
6
2022
medline:
9
7
2022
entrez:
24
6
2022
Statut:
epublish
Résumé
Successful regeneration requires the coordinated execution of multiple cellular responses to injury. In amputated zebrafish fins, mature osteoblasts dedifferentiate, migrate towards the injury, and form proliferative osteogenic blastema cells. We show that osteoblast migration is preceded by cell elongation and alignment along the proximodistal axis, which require actomyosin, but not microtubule (MT) turnover. Surprisingly, osteoblast dedifferentiation and migration can be uncoupled. Using pharmacological and genetic interventions, we found that NF-ĸB and retinoic acid signalling regulate dedifferentiation without affecting migration, while the complement system and actomyosin dynamics affect migration but not dedifferentiation. Furthermore, by removing bone at two locations within a fin ray, we established an injury model containing two injury sites. We found that osteoblasts dedifferentiate at and migrate towards both sites, while accumulation of osteogenic progenitor cells and regenerative bone formation only occur at the distal-facing injury. Together, these data indicate that osteoblast dedifferentiation and migration represent generic injury responses that are differentially regulated and can occur independently of each other and of regenerative growth. We conclude that successful fin bone regeneration appears to involve the coordinated execution of generic and regeneration-specific responses of osteoblasts to injury.
Identifiants
pubmed: 35748539
doi: 10.7554/eLife.77614
pii: 77614
pmc: PMC9259016
doi:
pii:
Substances chimiques
Zebrafish Proteins
0
Actomyosin
9013-26-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022, Sehring et al.
Déclaration de conflit d'intérêts
IS, HM, MH, AI, MH, GW No competing interests declared
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