Structure of the dengue virus RNA promoter.
NMR
UTR
dengue virus
flaviviruses
stem–loop A
Journal
RNA (New York, N.Y.)
ISSN: 1469-9001
Titre abrégé: RNA
Pays: United States
ID NLM: 9509184
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
received:
14
04
2022
accepted:
15
06
2022
pubmed:
25
6
2022
medline:
19
8
2022
entrez:
24
6
2022
Statut:
ppublish
Résumé
Dengue virus, a single-stranded positive sense RNA virus, is the most prevalent mosquito-borne pathogen in the world. Like all RNA viruses, it uses conserved structural elements within its genome to control essential replicative steps. A 70 nt stem-loop RNA structure (called SLA), found at the 5'-end of the genome of all flaviviruses, functions as the promoter for viral replication. This highly conserved structure interacts with the viral polymerase NS5 to initiate RNA synthesis. Here, we report the NMR structure of a monomeric SLA from dengue virus serotype 1, assembled to high-resolution from independently folded structural elements. The DENV1 SLA has an L-shaped structure, where the top and side helices are coaxially stacked, and the bottom helix is roughly perpendicular to them. Because the sequence is highly conserved among different flavivirus genomes, it is very likely that the three-dimensional fold and local structure of SLA are also conserved among flaviviruses and required for efficient replication. This work provides structural insight into the dengue promoter and provides the foundation for the discovery of new antiviral drugs that target this essential replicative step.
Identifiants
pubmed: 35750488
pii: rna.079197.122
doi: 10.1261/rna.079197.122
pmc: PMC9380747
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1210-1223Informations de copyright
© 2022 Sun and Varani; Published by Cold Spring Harbor Laboratory Press for the RNA Society.
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