Mesenchymal stromal cells cultured in physiological conditions sustain citrate secretion with glutamate anaplerosis.

Bone Marrow Cells Citrates / metabolism Glucose / metabolism Glutamic Acid / metabolism Humans Mesenchymal Stem Cells / metabolism
Citrate Glutamate Glutamine Hypoxia Mesenchymal stromal cells Metabolism Physiological medium Plasmax Primary cells Stable isotope tracing

Journal

Molecular metabolism
ISSN: 2212-8778
Titre abrégé: Mol Metab
Pays: Germany
ID NLM: 101605730

Informations de publication

Date de publication:
09 2022
Historique:
received: 14 03 2022
revised: 31 05 2022
accepted: 17 06 2022
pubmed: 26 6 2022
medline: 16 8 2022
entrez: 25 6 2022
Statut: ppublish

Résumé

Bone marrow mesenchymal stromal cells (MSCs) have immunomodulatory and regenerative potential. However, culture conditions govern their metabolic processes and therapeutic efficacy. Here we show that culturing donor-derived MSCs in Plasmax™, a physiological medium with the concentrations of nutrients found in human plasma, supports their proliferation and stemness, and prevents the nutritional stress induced by the conventional medium DMEM. The quantification of the exchange rates of metabolites between cells and medium, untargeted metabolomics, stable isotope tracing and transcriptomic analysis, performed at physiologically relevant oxygen concentrations (1%O

Identifiants

DOI: 10.1016/j.molmet.2022.101532 PMID: 35752287 PMC: PMC9254159
pubmed: 35752287
pii: S2212-8778(22)00101-6
doi: 10.1016/j.molmet.2022.101532
pmc: PMC9254159
pii:
doi:

Substances chimiques

Citrates 0
Glutamic Acid 3KX376GY7L
Glucose IY9XDZ35W2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101532

Subventions

Organisme : Cancer Research UK
ID : C596/A17196
Pays : United Kingdom
Organisme : Cancer Research UK
ID : A31287
Pays : United Kingdom
Organisme : Cancer Research UK
ID : A23982
Pays : United Kingdom

Informations de copyright

Copyright © 2022 The Author(s). Published by Elsevier GmbH.. All rights reserved.

Auteurs

Giuseppe Taurino (G)

Laboratory of General Pathology, Dept. of Medicine and Surgery, University of Parma, 43125, Parma, Italy; MRH - Microbiome Research Hub, Parco Area delle Scienze 11/A, University of Parma, 43124 Parma, Italy.

Ruhi Deshmukh (R)

Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK.

Victor H Villar (VH)

Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK.

Martina Chiu (M)

Laboratory of General Pathology, Dept. of Medicine and Surgery, University of Parma, 43125, Parma, Italy.

Robin Shaw (R)

Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK.

Ann Hedley (A)

Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK.

Engy Shokry (E)

Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK.

David Sumpton (D)

Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK.

Erica Dander (E)

Centro Ricerca Tettamanti, Pediatric Dept., University of Milano-Bicocca, Fondazione MBBM, Monza, 20900, Italy.

Giovanna D'Amico (G)

Centro Ricerca Tettamanti, Pediatric Dept., University of Milano-Bicocca, Fondazione MBBM, Monza, 20900, Italy.

Ovidio Bussolati (O)

Laboratory of General Pathology, Dept. of Medicine and Surgery, University of Parma, 43125, Parma, Italy; MRH - Microbiome Research Hub, Parco Area delle Scienze 11/A, University of Parma, 43124 Parma, Italy. Electronic address: ovidio.bussolati@unipr.it.

Saverio Tardito (S)

Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, G61 1BD, UK; Institute of Cancer Sciences, University of Glasgow, Glasgow, G61 1QH, UK. Electronic address: s.tardito@beatson.gla.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Systèmes sanguin et immunitaire › Système hématopoïétique › Cellules de la moelle osseuse › Mesenchymal stromal cells cultured in...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Triacides carboxyliques › Citrates › Mesenchymal stromal cells cultured in...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Mesenchymal stromal cells cultured in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Acides aminés excitateurs › Acide glutamique › Mesenchymal stromal cells cultured in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Mesenchymal stromal cells cultured in...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Mesenchymal stromal cells cultured in...