Clonal structure, stability and dynamics of human memory B cells and circulating plasmablasts.
Journal
Nature immunology
ISSN: 1529-2916
Titre abrégé: Nat Immunol
Pays: United States
ID NLM: 100941354
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
received:
17
01
2022
accepted:
26
04
2022
pubmed:
28
6
2022
medline:
15
7
2022
entrez:
27
6
2022
Statut:
ppublish
Résumé
Memory B cells persist for a lifetime and rapidly differentiate into antibody-producing plasmablasts and plasma cells upon antigen re-encounter. The clonal relationship and evolution of memory B cells and circulating plasmablasts is not well understood. Using single-cell sequencing combined with isolation of specific antibodies, we found that in two healthy donors, the memory B cell repertoire was dominated by large IgM, IgA and IgG2 clonal families, whereas IgG1 families, including those specific for recall antigens, were of small size. Analysis of multiyear samples demonstrated stability of memory B cell clonal families and revealed that a large fraction of recently generated plasmablasts was derived from long-term memory B cell families and was found recurrently. Collectively, this study provides a systematic description of the structure, stability and dynamics of the human memory B cell pool and suggests that memory B cells may be active at any time point in the generation of plasmablasts.
Identifiants
pubmed: 35761085
doi: 10.1038/s41590-022-01230-1
pii: 10.1038/s41590-022-01230-1
pmc: PMC9276532
doi:
Substances chimiques
Immunoglobulin G
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1076-1085Informations de copyright
© 2022. The Author(s).
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