Co-immunoprecipitation of Protein Complexes from Different Subcellular Compartments in Vasculogenic Mimicry Studies.
Immunoprecipitation
Protein complexes
VE-cadherin
Vasculogenic mimicry
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
30
6
2022
pubmed:
1
7
2022
medline:
6
7
2022
Statut:
ppublish
Résumé
Aberrant extravascular expression of VE-cadherin has been observed in metastasis associated with vasculogenic mimicry (VM); we have recently shown that in VM prone cells VE-cadherin (mainly in the form of phospho-VE-cadherin in Y658) is in part located in the cell nucleus, which associates with p120-catenin and the transcription factor kaiso allowing increased plasticity that potentiates VM development in malignant cells. In this chapter, we describe the protocol to analyze protein-protein interactions in subcellular fractions with particular focus in VE-cadherin. The verification of the subcellular interactome of VE-cadherin and other key proteins involved in VM shed light to novel functions of endothelial proteins aberrantly expressed in tumor cells and their consequences in cell plasticity during VM development.
Identifiants
pubmed: 35771419
doi: 10.1007/978-1-0716-2403-6_7
doi:
Substances chimiques
Cadherins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
61-72Informations de copyright
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.
Références
Folberg R, Maniotis AJ (2004) Vasculogenic mimicry. APMIS 112:508–525. https://doi.org/10.1111/j.1600-0463.2004.apm11207-0810.x
doi: 10.1111/j.1600-0463.2004.apm11207-0810.x
pubmed: 15563313
Maniotis AJ, Folberg R, Hess A et al (1999) Vascular channel formation by human melanoma cells in vivo and in vitro: Vasculogenic mimicry. Am J Pathol 155:739–752. https://doi.org/10.1016/s0002-9440(10)65173-5
doi: 10.1016/s0002-9440(10)65173-5
pubmed: 10487832
pmcid: 1866899
Leach L, Clark P, Lampugnani MG et al (1993) Immunoelectron characterisation of the inter-endothelial junctions of human term placenta. J Cell Sci 104:1073–1081
doi: 10.1242/jcs.104.4.1073
Giannotta M, Trani M, Dejana E (2013) VE-cadherin and endothelial adherens junctions: active guardians of vascular integrity. Dev Cell 26:441–454. https://doi.org/10.1016/j.devcel.2013.08.020
doi: 10.1016/j.devcel.2013.08.020
pubmed: 24044891
Delgado-Bellido D, Serrano-Saenz S, Fernández-Cortés M et al (2017) Vasculogenic mimicry signaling revisited: focus on non-vascular VE-cadherin. Mol Cancer 16:1476–4598. https://doi.org/10.1186/s12943-017-0631-x
doi: 10.1186/s12943-017-0631-x
Delgado-Bellido D, Fernández-Cortés M, Rodríguez MI et al (2018) VE-cadherin promotes vasculogenic mimicry by modulating kaiso-dependent gene expression. Cell Death Differ 26:348–361. https://doi.org/10.1038/s41418-018-0125-4
doi: 10.1038/s41418-018-0125-4
pubmed: 29786069
pmcid: 6329820
Delgado-Bellido D, Bueno-Galera C, López-Jiménez L et al (2020) Endothelial phosphatase VE-PTP participates in vasculogenic mimicry by preventing autophagic degradation of VE-cadherin. Front Oncol 10:2234–943X. https://doi.org/10.3389/fonc.2020.00018
doi: 10.3389/fonc.2020.00018
Hess AR, Seftor EA, Gruman LM et al (2006) VE-cadherin regulates EphA2 in aggressive melanoma cells through a novel signaling pathway: implications for vasculogenic mimicry. Cancer Biol Ther 5:228–233. https://doi.org/10.4161/cbt.5.2.2510
doi: 10.4161/cbt.5.2.2510
pubmed: 16481735
Kirschmann DA, Seftor EA, Hardy KM et al (2012) Molecular pathways: Vasculogenic mimicry in tumor cells: diagnostic and therapeutic implications. Clin Cancer Res 15(18):2726–2732. https://doi.org/10.1158/1078-0432.CCR-11-3237
doi: 10.1158/1078-0432.CCR-11-3237
Su YJ, Chang YW, Lin WH et al (2015) An aberrant nuclear localization of E-cadherin is a potent inhibitor of Wnt/ß-catenin-elicited promotion of the cancer stem cell phenotype. Oncogenesis 4:e157. https://doi.org/10.1038/oncsis.2015.17
doi: 10.1038/oncsis.2015.17
pubmed: 26075748
pmcid: 4491612
McCrea PD, Maher MT, Gottardi CJ (2015) Nuclear signaling from cadherin adhesion complexes. Curr Top Dev Biol 112:129–196. https://doi.org/10.1016/bs.ctdb.2014.11.018
doi: 10.1016/bs.ctdb.2014.11.018
pubmed: 25733140
pmcid: 4410048
He Y, Davies CM, Harrington BS et al (2019) CDCP1 enhances Wnt signaling in colorectal cancer promoting nuclear localization of β-catenin and E-cadherin. Oncogene 39:219–233. https://doi.org/10.1038/s41388-019-0983-3
doi: 10.1038/s41388-019-0983-3
pubmed: 31471585
Aebersold R, Mann M (2003) Mass spectrometry-based proteomics. Nature 422:198–207. https://doi.org/10.1038/nature01511
doi: 10.1038/nature01511
pubmed: 12634793
Lin JS, Lai EM (2017) Protein–protein interactions: co-immunoprecipitation. Methods Mol Biol 1615:211–219. https://doi.org/10.1007/978-1-4939-7033-9_17
doi: 10.1007/978-1-4939-7033-9_17
pubmed: 28667615
Rockstroh M, Müller SA, Jende C, et al (2011) Cell fractionation – an important tool for compartment proteomics. J Integr OMICS. 135–143. https://doi.org/10.5584/JIOMICS.V1I1.52
Liu X, Fagotto F (2011) A method to separate nuclear, cytosolic, and membrane-associated signaling molecules in cultured cells. Sci Signal 4:203-pl2. https://doi.org/10.1126/scisignal.2002373
doi: 10.1126/scisignal.2002373
Lowry OH, Rosebrough NJ, Farr AL et al (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 93:265–275
doi: 10.1016/S0021-9258(19)52451-6
Kruger NJ (1994) The Bradford method for protein quantitation. Methods Mol Biol 32:9–15. https://doi.org/10.1385/0-89603-268-X:9
doi: 10.1385/0-89603-268-X:9
pubmed: 7951753
Johnson M (2013) Western blot protocol. Mater Methods 3. https://doi.org/10.13070/MM.EN.3.195
Wessel F, Winderlich M, Holm M et al (2014) Leukocyte extravasation and vascular permeability are each controlled in vivo by different tyrosine residues of VE-cadherin. Nat Immunol 15:223–230. https://doi.org/10.1038/ni.2824
doi: 10.1038/ni.2824
pubmed: 24487320
Lambeng N, Wallez Y, Rampon C et al (2005) Vascular endothelial-cadherin tyrosine phosphorylation in angiogenic and quiescent adult tissues. Circ Res 96:384–391. https://doi.org/10.1161/01.RES.0000156652.99586.9f
doi: 10.1161/01.RES.0000156652.99586.9f
pubmed: 15662029
pmcid: 2798002
Sinha S, Field JJ, Miller JH (2017) Use of substitute Nonidet P-40 nonionic detergents in intracellular tubulin polymerization assays for screening of microtubule targeting agents. Biochem Cell Biol 95:379–384. https://doi.org/10.1139/bcb-2016-0141
doi: 10.1139/bcb-2016-0141
pubmed: 28177759
Koley D, Bard AJ (2010) Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM). Proc Natl Acad Sci 107:16783–16787. https://doi.org/10.1073/pnas.1011614107
doi: 10.1073/pnas.1011614107
pubmed: 20837548
pmcid: 2947864