Successful Isolation of Leishmania RNA Virus (LRV) from Leishmania major in a Cutaneous Leishmaniasis Focus in Central Iran: An Update on Cases.
Cutaneous Leishmaniasis
Iran
Kashan
Leishmania RNA virus
RdRp
Journal
Acta parasitologica
ISSN: 1896-1851
Titre abrégé: Acta Parasitol
Pays: Switzerland
ID NLM: 9301947
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
18
01
2022
accepted:
10
05
2022
pubmed:
1
7
2022
medline:
26
8
2022
entrez:
30
6
2022
Statut:
ppublish
Résumé
Cutaneous leishmaniasis (CL) is a major vector-borne disease that affects people globally, including Iran. Different factors are associated with leishmaniasis pathogenicity; recently, a link of the possible relationship between Leishmania RNA Virus (LRV) and disease severity was proposed, especially in the New World leishmaniasis (NWL). This study was aimed to investigate the presence of LRV2 in Leishmania isolates in Aran o Bidgol, Isfahan province. Samples were collected from 110 CL-suspected patients referred to the health center. In this study, we aimed to investigate CL cases (parasitologically and clinically), identify Leishmania species (by ITS1-PCR-RFLP), and finally detection of LRV2 (by RdRp-semi-nested PCR). Parasitological methods showed 60 positive cases, based on the HaeIII enzyme restriction profile, 59 cases were caused by L. major and 1 case by L. tropica. Our project is the first study on LRV2 isolation in Aran o Bidgol city and the LRV was successfully detected from a single L. major isolated in a women's hand lesion. Using BLAST, 94.8-100% similarity was observed in the RdRp sequence of current LRV isolate with those available in GenBank from Iran or overseas. L. major was the main cause of CL in Aran o Bidgol, although L. tropica is also present in a much lower proportion in the area. This is the first report on the presence of LRV2 in Aran o Bidgol and the fifth in Iran.
Identifiants
pubmed: 35773567
doi: 10.1007/s11686-022-00575-9
pii: 10.1007/s11686-022-00575-9
pmc: PMC9245859
doi:
Substances chimiques
RNA-Dependent RNA Polymerase
EC 2.7.7.48
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1290-1298Subventions
Organisme : Shahid Beheshti University of Medical Sciences
ID : 20101
Informations de copyright
© 2022. The Author(s) under exclusive licence to Witold Stefański Institute of Parasitology, Polish Academy of Sciences.
Références
World Health Organization. Fact sheets on leishmaniasis. Available at: https://www.who.int/news-room/fact-sheets/detail/leishmaniasis
Ghatee MA, Taylor WR, Karamian M (2020) The geographical distribution of cutaneous leishmaniasis causative agents in Iran and its neighboring countries a review. Front Public Health 8:11. https://doi.org/10.3389/fpubh.2020.00011
doi: 10.3389/fpubh.2020.00011
pubmed: 32133334
pmcid: 7039857
Mohebali M (2013) Visceral leishmaniasis in Iran: review of the epidemiological and clinical features. Iran J Parasitol 8(3):348–58
pubmed: 24454426
pmcid: 3887234
Norouzinezhad F, Ghaffari F, Norouzinejad A, Kaveh F, Gouya MM (2016) Cutaneous leishmaniasis in Iran: results from an epidemiological study in urban and rural provinces. Asian Pac J Trop Biomed 6(7):614–619. https://doi.org/10.1016/j.apjtb.2016.05.005
doi: 10.1016/j.apjtb.2016.05.005
Yaghoobi-Ershadi M (2012) Phlebotomine sand flies (Diptera: Psychodidae) in Iran and their role on Leishmania transmission. J Arthropod Borne Dis 6(1):1–17
pubmed: 23293774
pmcid: 3528173
Hajjaran H, Saberi R, Borjian A, Fakhar M, Hosseini SA, Ghodrati S, Mohebali M (2021) The geographical distribution of human cutaneous and visceral leishmania species identified by molecular methods in Iran: a systematic review with meta-analysis. Front Public Health 9:835. https://doi.org/10.3389/fpubh.2021.661674
doi: 10.3389/fpubh.2021.661674
Sabzevari S, Teshnizi SH, Shokri A, Bahrami F, Kouhestani F (2021) Cutaneous leishmaniasis in Iran: a systematic review and meta-analysis. Microb Pathog 152:104721. https://doi.org/10.1016/j.micpath.2020.104721
doi: 10.1016/j.micpath.2020.104721
pubmed: 33539962
Shiee MR, Mohebali M, Doroodgar A, Teimouri A, Afzali H, Shirzadi MR (2012) A molecular and parasitological survey on cutaneous leishmaniasis patients from historical city of Kashan in Isfahan province, center of Iran. Asian Pac J Trop Dis 2(6):421–5. https://doi.org/10.1016/S2222-1808(12)60093-0
doi: 10.1016/S2222-1808(12)60093-0
Sunter J, Gull K (2017) Shape, form, function and leishmania pathogenicity: from textbook descriptions to biological understanding. Open Biol 7(9):170165. https://doi.org/10.1098/rsob.170165
doi: 10.1098/rsob.170165
pubmed: 28903998
pmcid: 5627057
Grybchuk D, Kostygov AY, Macedo DH, d’Avila-Levy CM, Yurchenko V (2018) RNA viruses in trypanosomatid parasites: a historical overview. Mem Inst Oswaldo Cruz 113(4):e170487. https://doi.org/10.1590/0074-02760170487
doi: 10.1590/0074-02760170487
pubmed: 29513877
pmcid: 5851034
de Carvalho RVH, Lima-Junior DS, da Silva MVG, Dilucca M, Rodrigues TS, Horta CV et al (2019) Leishmania RNA virus exacerbates leishmaniasis by subverting innate immunity Via TLR3-mediated NLRP3 inflammasome inhibition. Nat Commun 10(1):5273. https://doi.org/10.1038/s41467-019-13356-2
doi: 10.1038/s41467-019-13356-2
pubmed: 31754185
pmcid: 6872735
Bourreau E, Ginouves M, Prevot G, Hartley MA, Gangneux JP, Robert-Gangneux F et al (2016) Presence of leishmania RNA virus 1 in leishmania guyanensis increases the risk of first-line treatment failure and symptomatic relapse. J Infect Dis 213(1):105–11. https://doi.org/10.1093/infdis/jiv355
doi: 10.1093/infdis/jiv355
pubmed: 26123564
Hartley MA, Ronet C, Zangger H, Beverley SM, Fasel N (2012) Leishmania RNA virus: when the host pays the toll. Front Cell Infect Microbiol 2:99. https://doi.org/10.3389/fcimb.2012.00099
doi: 10.3389/fcimb.2012.00099
pubmed: 22919688
pmcid: 3417650
Scheffter SM, Ro YT, Chung IK, Patterson JL (1995) The complete sequence of Leishmania RNA virus LRV2-1, a virus of an old world parasite strain. Virolology 212(1):84–90. https://doi.org/10.1006/viro.1995.1456
doi: 10.1006/viro.1995.1456
Widmer G, Dooley S (1995) Phylogenetic analysis of leishmania RNA virus and leishmania suggests ancient virus-parasite association. Nucleic Acids Res 23(12):2300–4. https://doi.org/10.1093/nar/23.12.2300
doi: 10.1093/nar/23.12.2300
pubmed: 7610059
pmcid: 307021
Adaui V, Lye LF, Akopyants NS, Zimic M, Llanos-Cuentas A, Garcia L et al (2016) Association of the endobiont double-stranded RNA virus LRV1 with treatment failure for human leishmaniasis caused by Leishmania braziliensis in Peru and Bolivia. J Infect Dis 213(1):112–21. https://doi.org/10.1093/infdis/jiv354
doi: 10.1093/infdis/jiv354
pubmed: 26123565
Ives A, Ronet C, Prevel F, Ruzzante G, Fuertes-Marraco S, Schutz F et al (2011) Leishmania RNA virus controls the severity of mucocutaneous leishmaniasis. Sci 331(6018):775–8. https://doi.org/10.1126/science.1199326
doi: 10.1126/science.1199326
Kariyawasam R, Lau R, Valencia BM, Llanos-Cuentas A, Boggild AK (2020) Leishmania RNA virus 1 (LRV-1) in Leishmania (Viannia) braziliensis isolates from Peru: a description of demographic and clinical correlates. Am J Trop Med Hyg. 102(2):280–285. https://doi.org/10.4269/ajtmh.19-0147
doi: 10.4269/ajtmh.19-0147
pubmed: 31837129
Kariyawasam R, Mukkala AN, Lau R, Valencia BM, Llanos-Cuentas A, Boggild AK (2019) Virulence factor RNA transcript expression in the Leishmania Viannia subgenus: influence of species, isolate source, and Leishmania RNA virus-1. Trop Med Health 47:25. https://doi.org/10.1186/s41182-019-0153-x
doi: 10.1186/s41182-019-0153-x
pubmed: 31007536
pmcid: 6458769
Zangger H, Hailu A, Desponds C, Lye LF, Akopyants NS, Dobson DE et al (2014) Leishmania aethiopica field isolates bearing an endosymbiontic dsRNA virus induce pro-inflammatory cytokine response. PLoS Negl Trop Dis 8(4):e2836. https://doi.org/10.1371/journal.pntd.0002836
doi: 10.1371/journal.pntd.0002836
pubmed: 24762979
pmcid: 3998932
Hajjaran H, Mahdi M, Mohebali M, Samimi-Rad K, Ataei-Pirkooh A, Kazemi-Rad E et al (2016) Detection and molecular identification of Leishmania RNA virus (LRV) in Iranian Leishmania species. Arch Virol 161(12):3385–90. https://doi.org/10.1007/s00705-016-3044-z
doi: 10.1007/s00705-016-3044-z
pubmed: 27604119
Saberi R, Fakhar M, Hajjaran H, Ataei-Pirkooh A, Mohebali M, Taghipour N et al (2020) Presence and diversity of Leishmania RNA virus in an old zoonotic cutaneous leishmaniasis focus, northeastern Iran: haplotype and phylogenetic based approach. Int J Infect Dis 101:6–13. https://doi.org/10.1016/j.ijid.2020.08.033
doi: 10.1016/j.ijid.2020.08.033
pubmed: 32947050
Nalcaci M, Karakus M, Yilmaz B, Demir S, Ozbilgin A, Ozbel Y et al (2019) Detection of Leishmania RNA virus 2 in Leishmania species from Turkey. Trans R Soc Trop Med Hyg 113(7):410–7. https://doi.org/10.1093/trstmh/trz023
doi: 10.1093/trstmh/trz023
pubmed: 31034027
Kleschenko Y, Grybchuk D, Matveeva NS, Macedo DH, Ponirovsky EN, Lukashev AN et al (2019) Molecular characterization of Leishmania RNA virus 2 in Leishmania major from Uzbekistan. Genes (Basel) 10(10):830. https://doi.org/10.3390/genes10100830
doi: 10.3390/genes10100830
Rasti S, Delavari M, Arani TS, Mousavi SG (2018) Epidemiological and clinical study on the cutaneous leishmaniasis in Aran and Bidgol, center of Iran. Int Arch Health Sci. 5:72–5. https://doi.org/10.4103/iahs.iahs_26_18
doi: 10.4103/iahs.iahs_26_18
Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W et al (2003) PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis. 47(1):349–58. https://doi.org/10.1016/s0732-8893(03)00093-2
doi: 10.1016/s0732-8893(03)00093-2
pubmed: 12967749
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 33(7):1870–1874. https://doi.org/10.1093/molbev/msw054
doi: 10.1093/molbev/msw054
pubmed: 27004904
pmcid: 8210823
Mokni M (2019) Cutaneous leishmaniasis. Ann Dermatol Venereol 146(3):232–46. https://doi.org/10.1016/j.annder.2019.02.002
doi: 10.1016/j.annder.2019.02.002
pubmed: 30879803
Hajjaran H, Mohebali M, Mamishi S, Vasigheh F, Oshaghi MA, Naddaf SR et al (2013) Molecular identification and polymorphism determination of cutaneous and visceral leishmaniasis agents isolated from human and animal hosts in Iran. Biomed Res Int 2013:789326. https://doi.org/10.1155/2013/789326
doi: 10.1155/2013/789326
pubmed: 24286085
pmcid: 3826333
Saberi R, Moin-Vaziri V, Hajjaran H, Niyyati M, Taghipour N, Kheirandish F et al (2018) Identification of Leishmania species using N-acetylglucosamine-1-phosphate transferase gene in a zoonotic cutaneous leishmaniasis focus of Iran. J Vector Borne Dis. 55(1):14–9. https://doi.org/10.4103/0972-9062.234621
doi: 10.4103/0972-9062.234621
pubmed: 29916443
Behravan M, Moin-Vaziri V, Haghighi A, Rahbarian N, Taghipour N, Abadi A et al (2017) Molecular identification of Leishmania species in a re-emerged focus of cutaneous leishmaniasis in Varamin district. Iran. J Arthropod Borne Dis. 11(1):124–31
Bisetegn H, Zeleke AJ, Gadisa E, Shumie G, Damte D, Fenta T et al (2020) Clinical, parasitological and molecular profiles of cutaneous leishmaniasis and its associated factors among clinically suspected patients attending Borumeda hospital, north-east Ethiopia. PLoS Negl Trop Dis. 14(8):e0008507. https://doi.org/10.1371/journal.pntd.0008507
doi: 10.1371/journal.pntd.0008507
pubmed: 32841279
pmcid: 7473553
Bates PA (2007) Transmission of Leishmania metacyclic promastigotes by phlebotomine sand flies. Int J Parasitol. 37(10):1097–106. https://doi.org/10.1016/j.ijpara.2007.04.003
doi: 10.1016/j.ijpara.2007.04.003
pubmed: 17517415
pmcid: 2675784
Courtenay O, Peters NC, Rogers ME, Bern C (2017) Combining epidemiology with basic biology of sand flies, parasites, and hosts to iNform leishmaniasis transmission dynamics and control. PLoS Pathog. 13(10):e1006571. https://doi.org/10.1371/journal.ppat.1006571
doi: 10.1371/journal.ppat.1006571
pubmed: 29049371
pmcid: 5648254
Abtahi M, Eslami G, Cavallero S, Vakili M, Hosseini SS, Ahmadian S et al (2020) Relationship of Leishmania RNA virus (LRV) and treatment failure in clinical isolates of Leishmania major. BMC Res Notes. 13(1):126. https://doi.org/10.1186/s13104-020-04973-y
doi: 10.1186/s13104-020-04973-y
pubmed: 32178715
pmcid: 7074996
Farrokhi-Karibozorg M, Ghayour-Najafabadi Z, Hejazi SH, Ataei-Pirkooh A, Mohebali M, Teimouri P et al (2022) Molecular identification of Leishmania RNA virus in cutaneous leishmaniasis patients and rodent reservoirs in Isfahan province. Iran. Infect Genet Evol. 98:105222. https://doi.org/10.1016/j.meegid.2022.105222
doi: 10.1016/j.meegid.2022.105222
pubmed: 35066166
Saberi R, Fakhar M, Mohebali M, Anvari D, Gholami S (2019) Global status of synchronizing Leishmania RNA virus in Leishmania parasites: a systematic review with meta-analysis. Transbound Emerg Dis. 66(6):2244–51. https://doi.org/10.1111/tbed.13316
doi: 10.1111/tbed.13316
pubmed: 31376334