Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae.

Membrane Proteins / metabolism Protein Folding Protein Processing, Post-Translational Protein Transport Recombinant Proteins / metabolism Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics
Chromatography Membrane protein Protein production Purification Saccharomyces cerevisiae

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 30 6 2022
pubmed: 1 7 2022
medline: 6 7 2022
Statut: ppublish

Résumé

Membrane protein (MP) functional and structural characterization requires large quantities of high-purity protein for downstream studies. Barriers to MP characterization include ample overexpression, solubilization, and purification of target proteins while maintaining native activity and structure. These barriers can be overcome by utilizing an efficient purification protocol in a high-yield eukaryotic expression system such as Saccharomyces cerevisiae. S. cerevisiae offers improved protein folding and posttranslational modifications compared to prokaryotic expression systems. This chapter contains practices used to overcome barriers of solubilization and purification using S. cerevisiae that are broadly applicable to diverse membrane associated, and membrane integrated, protein targets.

Identifiants

DOI: 10.1007/978-1-0716-2368-8_8 PMID: 35773581 PMC: PMC9400948
pubmed: 35773581
doi: 10.1007/978-1-0716-2368-8_8
pmc: PMC9400948
mid: NIHMS1760360
doi:

Substances chimiques

Membrane Proteins 0
Recombinant Proteins 0
Saccharomyces cerevisiae Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

143-173

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM118599
Pays : United States

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Curr Opin Struct Biol. 1997 Oct;7(5):697-701
pubmed: 9345629
Methods Enzymol. 2003;372:65-86
pubmed: 14610807
Curr Opin Struct Biol. 2009 Aug;19(4):379-85
pubmed: 19682888
Biophys J. 2004 Feb;86(2):1082-8
pubmed: 14747343
Methods Mol Biol. 2007;400:571-85
pubmed: 17951760
J Am Chem Soc. 2016 Mar 23;138(11):3789-96
pubmed: 26966956
Proc Natl Acad Sci U S A. 2006 Nov 21;103(47):17707-12
pubmed: 17098868
Methods Mol Biol. 2015;1329:259-70
pubmed: 26427691
Biochemistry. 2005 Apr 5;44(13):5196-206
pubmed: 15794657
Am J Physiol. 1993 Nov;265(5 Pt 1):L421-9
pubmed: 8238529
Protein Sci. 2003 Apr;12(4):875-84
pubmed: 12649445
Proc Natl Acad Sci U S A. 2002 Oct 15;99(21):13413-8
pubmed: 12370422
Biochim Biophys Acta Gen Subj. 2019 Feb;1863(2):437-455
pubmed: 30419284
J Chromatogr A. 1996 Aug 30;743(1):43-50
pubmed: 8817873
FEBS Lett. 1985 Nov 18;192(2):220-4
pubmed: 3840746
Anal Biochem. 1987 Apr;162(1):47-64
pubmed: 3605596
J Biol Chem. 2001 Aug 31;276(35):32403-6
pubmed: 11432878
J Am Chem Soc. 2004 Apr 28;126(16):5048-9
pubmed: 15099070
Angew Chem Int Ed Engl. 2018 Mar 5;57(11):2948-2952
pubmed: 29377441
Biochim Biophys Acta Biomembr. 2020 May 1;1862(5):183192
pubmed: 31945320
Science. 2004 Sep 10;305(5690):1587-94
pubmed: 15361618
PLoS Biol. 2003 Dec;1(3):E72
pubmed: 14691544
J Struct Biol. 2003 Apr;142(1):108-32
pubmed: 12718924
Biochem J. 1993 Jan 1;289 ( Pt 1):215-9
pubmed: 8424760
Novartis Found Symp. 2002;245:51-61; discussion 61-5, 165-8
pubmed: 12027015
Protein Sci. 2002 Sep;11(9):2067-79
pubmed: 12192063
Methods Mol Biol. 2011;746:135-64
pubmed: 21607856
Protein Sci. 2005 Oct;14(10):2638-53
pubmed: 16195551
Cell Mol Life Sci. 2006 Jan;63(1):36-51
pubmed: 16314922
Biochim Biophys Acta Biomembr. 2019 Feb 1;1861(2):466-477
pubmed: 30444973
Sci Rep. 2020 Jul 22;10(1):12199
pubmed: 32699228
Q Rev Biophys. 2007 Aug;40(3):191-285
pubmed: 18078545
Biotechnol Bioeng. 2016 Jan;113(1):130-40
pubmed: 26174988
Annu Rev Biophys Biomol Struct. 2007;36:307-27
pubmed: 17284163
J Am Chem Soc. 2013 Feb 6;135(5):1919-25
pubmed: 23294159
Protein Expr Purif. 2011 Aug;78(2):139-42
pubmed: 21539919
Biol Chem. 2015 Aug;396(8):903-15
pubmed: 25781680
Methods Mol Biol. 2017;1607:117-141
pubmed: 28573571
Methods Enzymol. 1974;32:513-39
pubmed: 4614005
Nanoscale. 2017 Jan 5;9(2):754-763
pubmed: 27976759
Nature. 2005 Dec 1;438(7068):633-8
pubmed: 16319884
Chem Rev. 2004 Aug;104(8):3587-606
pubmed: 15303829
Nat Protoc. 2008;3(5):784-98
pubmed: 18451787
J Bioenerg Biomembr. 1996 Feb;28(1):13-27
pubmed: 8786233
Nat Commun. 2018 Feb 7;9(1):544
pubmed: 29416037
Protein Sci. 2008 Mar;17(3):466-72
pubmed: 18218713
Comb Chem High Throughput Screen. 2008 Jul;11(6):439-62
pubmed: 18673272
J Pharm Sci. 2017 Aug;106(8):2178-2186
pubmed: 28479353
Chemistry. 2013 Nov 11;19(46):15645-51
pubmed: 24123610
Nat Struct Mol Biol. 2008 Jun;15(6):619-25
pubmed: 18500352
PLoS One. 2018 Jun 13;13(6):e0198832
pubmed: 29897975
J Mol Biol. 2002 Feb 8;316(1):1-6
pubmed: 11829498
Methods Enzymol. 2015;557:393-416
pubmed: 25950975
Methods. 2004 Nov;34(3):364-72
pubmed: 15325654
Anal Chem. 1994 Jun 15;66(12):595R-620R
pubmed: 8092479
Annu Rev Biochem. 2010;79:737-75
pubmed: 20307193
Protein Expr Purif. 2003 Jan;27(1):109-14
pubmed: 12509992

Auteurs

Landon Haslem (L)

Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Marina Brown (M)

Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Xin A Zhang (XA)

Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.
Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Jennifer M Hays (JM)

Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA.

Franklin A Hays (FA)

Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. Franklin-Hays@ouhsc.edu.
Stephenson Cancer Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA. Franklin-Hays@ouhsc.edu.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Purification of Membrane Proteins...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › Purification of Membrane Proteins...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Modification traductionnelle des protéines › Maturation post-traductionnelle des protéines › Purification of Membrane Proteins...
questionsmedicales.fr › Métabolisme › Transport biologique › Transport des protéines › Purification of Membrane Proteins...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Purification of Membrane Proteins...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Purification of Membrane Proteins...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines fongiques › Protéines de Saccharomyces cerevisiae › Purification of Membrane Proteins...