Effect of dihydrotestosterone on melatonin secretion and the expression of melatonin receptors and apoptosis-related factors in sheep epididymides.
Animals
Apoptosis
Caspase 3
/ metabolism
Dihydrotestosterone
/ metabolism
Epididymis
/ metabolism
Flutamide
/ metabolism
Ligases
/ metabolism
Male
Melatonin
/ metabolism
RNA, Messenger
/ metabolism
Receptors, Androgen
/ genetics
Receptors, Melatonin
/ metabolism
Semen
/ chemistry
Sheep
bcl-2-Associated X Protein
/ metabolism
apoptosis
dihydrotestosterone
epididymis
melatonin
sheep
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
received:
27
12
2021
accepted:
28
06
2022
pubmed:
2
7
2022
medline:
12
10
2022
entrez:
1
7
2022
Statut:
ppublish
Résumé
Melatonin (MEL) is involved in homeostasis of the epididymis lumen environment. Dihydrotestosterone (DHT) partakes in the development of gonads and organs in male animals. However, whether MEL secretion, the expression of its receptors, MT1 and MT2, and sheep epididymal epithelial cell apoptosis is regulated by DHT remains unclear. In this study, we used immunohistochemical staining to detect the distribution patterns of DHT synthetases [5α-reductase (5α-red)] and its androgen receptor (AR) in sheep epididymides. 5α-red1, 5α-red2 and AR were positively expressed in sperm, epididymal epithelial cells, and the smooth muscle cells of the caput, corpus and cauda regions of the epididymis. DHT concentration and the expression levels of 5α-red and AR in the caput, corpus and cauda regions were measured by enzyme-linked immunosorbent assay, liquid chromatography-mass spectrometry, real-time quantitative polymerase chain reaction and western blot analysis. DHT concentration in the caput was significantly higher than those in corpus and cauda, probably because of the high expression of 5α-red2 in the caput and secretion and transport of DHT by the testicles. DHT inhibited MEL secretion, the expression of its membrane receptors and MEL synthetases in cultured sheep epididymal epithelial cells in vitro. In addition, the Bax/Bcl-2 ratio, ACT CASP3 and caspase-3 mRNA expression were also decreased. The decreasing effect was partially reversed after flutamide treatment. Therefore, DHT regulates sheep epididymal function by influencing MEL expression and apoptosis-related factors. This study provides basic data for further research on the reproductive physiology of male animals.
Substances chimiques
RNA, Messenger
0
Receptors, Androgen
0
Receptors, Melatonin
0
bcl-2-Associated X Protein
0
Dihydrotestosterone
08J2K08A3Y
Flutamide
76W6J0943E
Caspase 3
EC 3.4.22.-
Ligases
EC 6.-
Melatonin
JL5DK93RCL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1244-1254Subventions
Organisme : Education science and technology innovation project of Gansu Province
Organisme : National Natural Science Foundation of China
Informations de copyright
© 2022 Wiley-VCH GmbH.
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