Transcriptomic analysis reveals the mechanism of host growth promotion by endophytic fungus of Rumex gmelinii Turcz.
Endophytic fungus
Growth promoting
Mechanism
Rumex gmelinii Turcz. (RGT)
Transcriptome
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
01 Jul 2022
01 Jul 2022
Historique:
received:
07
10
2021
accepted:
13
06
2022
revised:
11
06
2022
entrez:
1
7
2022
pubmed:
2
7
2022
medline:
7
7
2022
Statut:
epublish
Résumé
Rumex gmelinii Turcz. (RGT) is a medicinal plant of the genus Rumex, family Polygonaceae. Our research group isolated an endophytic fungus, Plectosphaerella cucumerina (Strain J-G) from RGT, which could significantly promote host growth when co-cultured with host seedlings. In this study, we used transcriptome analysis and verification experiments to explore the molecular mechanisms underlying this growth-promoting effect. We found that, during co-culture with Strain J-G, the expression of genes encoding key enzymes in amino acid metabolism and carbohydrate synthesis and metabolism were up-regulated in RGT tissue culture seedlings, providing additional substrate and energy for plant growth. In addition, the expression of genes encoding the responser of RGT seedlings to hormones, including auxin and cytokinin, were significantly enhanced, promoting plant growth and development. Furthermore, RGT seedling defense systems were mobilized by Strain J-G; therefore, more secondary metabolites and substances involved in stress resistance were produced, ensuring normal plant growth and metabolism. The research showed Strain J-G significantly promote the accumulation of biomass and effective components of RGT, which provide basis for its application. This research also provides a reference method for the study of growth-promoting mechanism of endophytic fungi.
Identifiants
pubmed: 35776209
doi: 10.1007/s00203-022-03072-9
pii: 10.1007/s00203-022-03072-9
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
443Subventions
Organisme : Heilongjiang Provincial Science and Technology Department
ID : C2016053
Organisme : Heilongjiang Provincial Science and Technology Department
ID : LH2020H093
Organisme : National Plan for Science, Technology and Innovation
ID : 2016yfc50500300
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Références
Asaf LS, Khan AL, Waqas M, Kang SM, Hamayun M, Lee IJ, Hussain A (2019) Growth-promoting bioactivities of Bipolaris sp. CSL-1 isolated from Cannabis sativa suggest a distinctive role in modifying host plant phenotypic plasticity and functions. Acta Physiol Plant 41:65–73. https://doi.org/10.1007/s11738-019-2852-7
doi: 10.1007/s11738-019-2852-7
Chen BB, Wang M, Hu YL, Lin ZP, Yu RM, Huang LQ (2011) A Preliminary study on the growth promoting effect of Rehmannia Glutinosa endophytic fungi. China J Chin Materia Med 36:1137–1140
Guo SX (2018) Research status and development trend of endophytic fungi in medicinal plants. Mycosystema 37:1–13
Jamshid T, Soghra M, Fatemeh SB, Hossein H (2018) Protective effects of Vitis vinifera (grapes) and one of its biologically active constituents, resveratrol, against natural and chemical toxicities: a comprehensive review. Phytotherap Res 32:2164–2190
doi: 10.1002/ptr.6168
Jia M, Chen L, Xin HL, Zheng CJ, Rahman K, Han T, Qin LP (2016) A friendly relationship between endophytic fungi and medicinal plants: a systematic review front. Microbial. https://doi.org/10.3389/fmicb.2016.00906
doi: 10.3389/fmicb.2016.00906
Katoch M, Pull S (2017) Endophytic fungi associated with Monarda citriodora, an aromatic and medicinal plant and their biocontrol potential. Pharm Biol 55:1528–1535. https://doi.org/10.1080/13880209.2017.1309054
doi: 10.1080/13880209.2017.1309054
pubmed: 28398103
pmcid: 6130496
Lamdan NL, Shalaby S, Ziv T, Kenerley CM, Horwitz BA (2015) Secretome of Trichoderma interacting with maize roots: role in induced systemic resistance. Mol Cell Proteomics 14:1054–1063. https://doi.org/10.1074/mcp.M114.046607
doi: 10.1074/mcp.M114.046607
pubmed: 25681119
pmcid: 4390251
Li JB, Ding CH, Zhang S, Guo H, Cui HH, Wang ZY (2018) Preliminary study on the growth promoting effect of endophytic Fungi from Rumex tomentosa. Inf Tradit Chin Med 35:6–9. https://doi.org/10.19656/j.cnki.1002-2406.180002
doi: 10.19656/j.cnki.1002-2406.180002
Liu KH, Ding XW, Narsing RMP, Zhang B, Zhang GZ, Liu FH, Liu BB, Xiao M, Li WJ (2017) Morphological and transcriptomic analysis reveals the osmoadaptive response of endophytic fungus Aspergillus montevidensis ZYD4 to high salt stress. Front Microbiol 8:1789–1799. https://doi.org/10.3389/fmicb.2017.01789
doi: 10.3389/fmicb.2017.01789
Liu J, Liu YM, Xv ZC, Wang ZY, Huang YL, Deng ZJ (2018) Studies on the diversity of endophytic fungi of Santalum Album and its antibacterial and growth-promoting properties. China J Chin Mater Med 43:3477–3483
Mastouri F, Björkman T, Harman GE (2012) Trichoderma harzianum enhances antioxidant defense of tomato seedlings and resistance to water deficit. Mol Plant Microbe Interact 25:1264–1271. https://doi.org/10.1094/MPMI-09-11-0240
doi: 10.1094/MPMI-09-11-0240
pubmed: 22512380
Morales-Sánchez V, Díaz CE, Trujillo E, Muoz R, Gonzalez-Coloma A (2021) Bioactive metabolites from the endophytic fungus Aspergillus sp SPH2. J Fungi 7:109
doi: 10.3390/jof7020109
Pang W, Fu P, Li X, Zhan Z, Yu S, Piao Z (2018) Identification and mapping of the clubroot resistance gene CRd in Chinese cabbage (Brassica rapa ssp. pekinensis). Front Plant Sci 9:653. https://doi.org/10.3389/fpls.2018.00653
doi: 10.3389/fpls.2018.00653
pubmed: 29868100
pmcid: 5968122
Ratnaweera PB, Nisansala JM, Jayasundara M, Suseema HHM, Herath D, Williams DE, Rajapaksha SU, Prabath Nishantha KMDW, EDilip de Silva, Andersen RJ (2020) Antifeedant, contact toxicity and oviposition deterrent effects of phyllostine acetate and phyllostine isolated from the endophytic fungus Diaporthe miriciae against Plutella xylostella larvae. Pest Management Science. 76(4):1541–1548. https://doi.org/10.1002/ps.5673
doi: 10.1002/ps.5673
pubmed: 31696600
Ren J, Sun C, Chen L, Hu J, Huang X, Liu X et al (2019) Exploring differentially expressed key genes related to development of follicle by RNA-seq in Peking ducks (Anas Platyrhynchos). PLoS ONE 14:e0209061. https://doi.org/10.1371/journal.pone.02090
doi: 10.1371/journal.pone.02090
pubmed: 31237879
pmcid: 6592512
Sun X, Wang N, Li P, Jiang ZY, Liu XY, Wang MC, Su ZZ, Zhang CL, Lin FC, Liang Y (2020) Endophytic fungus Falciphora oryzae promotes lateral root growth by producing indole derivatives after sensing plant signals. Plant Cell Environ 43:358–373. https://doi.org/10.1111/pce.13667
doi: 10.1111/pce.13667
pubmed: 31675439
Wang ZY, Zuo YM, Kang YH, Cong Y, Song XL (2005) Study on the chemical composition of Rumex gmelinii Turcz. (II). Chin Tradit Herb Drugs 11:1626–1627. https://doi.org/10.3321/j.issn:0253-2670.2005.11.011
doi: 10.3321/j.issn:0253-2670.2005.11.011
Wang ZY, Chen JM, Wang QB, Kang YH, Cong Y, Liu YX (2009) Study on the chemical composition of Rumex gmelinii Turcz. (IV). Chin Tradit Herb Drugs 40:1352–1355. https://doi.org/10.3321/j.issn:0253-2670,2009.09.003
doi: 10.3321/j.issn:0253-2670,2009.09.003
Wang XL, Huang LQ, Yuan Y, Cha LP (2015) Research progress on transcriptome of medicinal plants. China J Chin Mater Med 40:2055–5012. https://doi.org/10.4268/cjcmm20151101
doi: 10.4268/cjcmm20151101
Wang FM, Hu ZA, Liu XH, Feng JQ, Augsburger RA, Gutmann JL, Glickman GN (2018) Resveratrol represses tumor necrosis factorα/c-Jun N-terminal kinase signaling via autophagy in human dental pulp stem cells. Arch Oral Biol 97:116–121. https://doi.org/10.1016/j.archoralbio.2018.10.020
doi: 10.1016/j.archoralbio.2018.10.020
pubmed: 30384152
pmcid: 6927335
Waqas M, Khan AL, Kamran M, Hamayun M, Kang SM, Kim YH (2012) Endophytic fungi produce gibberellinsand indoleacetic acid and promotes host-plant growth during stress. Molecules 17:10754–10773. https://doi.org/10.3390/molecules170910754
doi: 10.3390/molecules170910754
pubmed: 22960869
pmcid: 6268353
Wonglom P, Ito SI, Sunpapao A (2020) Volatile organic compounds emitted from endophytic fungus Trichoderma asperellum T1 mediate antifungal activity, defense response and promote plant growth in lettuce (Lactuca sativa). Fungal Ecol 43:100867–100875. https://doi.org/10.1016/j.funeco.2019.100867
doi: 10.1016/j.funeco.2019.100867
Wu XL, Cui GL, Liu F, Li LY (2018) Isolation and molecular identification of endophytic fungi from Artemisia Annua and study on the growth promoting effect of Trichoderma Viride on Artemisia Annua. J Trop Subtrop Bot 26:56–64
Xing XK (2018) Endophytic fungal resources of medicinal plants: a treasure trove to be developed. Mycosystema 37:14–21
Yuan M, Tan SJ, Sun JG (2016) Isolation, identification and biological characteristics of endophytic nitrogen-fixing bacteria in rice and their effects on cadmium uptake by rice seedlings. Scientia Agricultura Sinica 49:3754–3768. https://doi.org/10.3864/j.issn.0578-1752.2016.19.008
doi: 10.3864/j.issn.0578-1752.2016.19.008
Zhang GQ, Zhao HP, Wang ZY, Cheng JR, Tang XM (2008) Research advances in chemical composition and pharmacological activities of Rumex. World Sci Technol-Mod Tradit Chin Med 10:86–93. https://doi.org/10.3969/j.issn.1674-3849.2008.05.020
doi: 10.3969/j.issn.1674-3849.2008.05.020
Zhang SB, Hou L, Pan Q, Wang XM, Cui QH, Tian JZ, Ma LY (2015) Research progress of high-throughput transcriptome of traditional chinese medicine. China J Chin Mater Med 39:1553–1558. https://doi.org/10.4268/cjcmm20140902
doi: 10.4268/cjcmm20140902
Zhou K, Wu WD, Song XQ, Zhou Y (2018) Screening and identification of endophytic fungi from Anoectochilus Roxburghii. J Trop Biol 9:440–444
Zhou LS, Tang S, Guo SX (2018) Endophytic fungus cladosporium sp effect on Salvia Miltiorrhiza growth and salvianolic acid content. Mycosystema 37:95–101