Development of a quantification method for routine analysis of glucosinolates and camalexin in brassicaceous small-sized samples by simultaneous extraction prior to liquid chromatography determination.
Absolute quantification
Camalexin
Glucosinolates
Simultaneous extraction
Small-sized samples
Journal
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
ISSN: 1873-376X
Titre abrégé: J Chromatogr B Analyt Technol Biomed Life Sci
Pays: Netherlands
ID NLM: 101139554
Informations de publication
Date de publication:
01 Aug 2022
01 Aug 2022
Historique:
received:
02
02
2022
revised:
13
06
2022
accepted:
14
06
2022
pubmed:
2
7
2022
medline:
20
7
2022
entrez:
1
7
2022
Statut:
ppublish
Résumé
Glucosinolates and camalexin are secondary metabolites that, as phytoanticipins and phytoalexins, play a crucial role in plant defence. The present work proposes an improved analytical method for routine analysis and quantification of glucosinolates and camalexin in brassicaceous small-sized samples by using the very specific desulfation process of glucosinolates analysis and the specificity of fluorescence detection for camalexin analysis. The approach is based on a simultaneous ultrasound-assisted extraction followed by a purification on an anion-exchange column. Final analyses are conducted by HPLC-UV-MS for desulfo-glucosinolates and HPLC coupled to a fluorescence detector (HPLC-FLD) for camalexin. The method is linear for glucosinolates (50-3500 µM) and camalexin (0.025-5 µg.mL
Identifiants
pubmed: 35777257
pii: S1570-0232(22)00252-5
doi: 10.1016/j.jchromb.2022.123348
pii:
doi:
Substances chimiques
Glucosinolates
0
Indoles
0
Thiazoles
0
camalexin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
123348Informations de copyright
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