Utilization of dielectric properties for assessment of liver ischemia-reperfusion injury in vivo and during machine perfusion.

Animals Liver / blood supply Liver Transplantation / methods Organ Preservation / methods Perfusion / methods Reperfusion Injury / diagnosis Swine

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
01 07 2022
Historique:
received: 22 01 2022
accepted: 13 06 2022
entrez: 1 7 2022
pubmed: 2 7 2022
medline: 8 7 2022
Statut: epublish

Résumé

There is a shortage of donor livers and patients consequently die on waiting lists worldwide. Livers are discarded if they are clinically judged to have a high risk of non-function following transplantation. With the aim of extending the pool of available donor livers, we assessed the condition of porcine livers by monitoring the microwave dielectric properties. A total of 21 livers were divided into three groups: control with no injury (CON), biliary injury by hepatic artery occlusion (AHEP), and overall hepatic injury by static cold storage (SCS). All were monitored for four hours in vivo, followed by ex vivo plurithermic machine perfusion (PMP). Permittivity data was modeled with a two-pole Cole-Cole equation, and dielectric properties from one-hour intervals were analyzed during in vivo and normothermic machine perfusion (NMP). A clear increasing trend in the conductivity was observed in vivo in the AHEP livers compared to the control livers. After four hours of NMP, separations in the conductivity were observed between the three groups. Our results indicate that dielectric relaxation spectroscopy (DRS) can be used to detect and differentiate liver injuries, opening for a standardized and reliable point of evaluation for livers prior to transplantation.

Identifiants

DOI: 10.1038/s41598-022-14817-3 PMID: 35778457 PMC: PMC9249774
pubmed: 35778457
doi: 10.1038/s41598-022-14817-3
pii: 10.1038/s41598-022-14817-3
pmc: PMC9249774
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

11183

Informations de copyright

© 2022. The Author(s).

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Auteurs

Jie Hou (J)

Department of Physics, University of Oslo, Sem Sælands vei 24, 0316, Oslo, Norway. jieho@fys.uio.no.
Department of Clinical and Biomedical Engineering, Oslo University Hospital, 0424, Oslo, Norway. jieho@fys.uio.no.

Olav Magnus Ivar Liavåg (OMI)

Section for Transplantation Surgery, Department of Transplantation Medicine, Oslo University Hospital, 0424, Oslo, Norway.
Institute of Clinical Medicine, University of Oslo, 0318, Oslo, Norway.

Ida Høy Færden (IH)

Institute of Clinical Medicine, University of Oslo, 0318, Oslo, Norway.
Department of Immunology, University of Oslo, 0372, Oslo, Norway.

Ørjan Grøttem Martinsen (ØG)

Department of Physics, University of Oslo, Sem Sælands vei 24, 0316, Oslo, Norway.
Department of Clinical and Biomedical Engineering, Oslo University Hospital, 0424, Oslo, Norway.

Tor Inge Tønnessen (TI)

Department of Emergencies and Critical Care, Oslo University Hospital, 0424, Oslo, Norway.
Institute of Clinical Medicine, University of Oslo, 0318, Oslo, Norway.

Pål-Dag Line (PD)

Section for Transplantation Surgery, Department of Transplantation Medicine, Oslo University Hospital, 0424, Oslo, Norway.
Institute of Clinical Medicine, University of Oslo, 0318, Oslo, Norway.

Morten Hagness (M)

Section for Transplantation Surgery, Department of Transplantation Medicine, Oslo University Hospital, 0424, Oslo, Norway.

Jan Olav Høgetveit (JO)

Department of Physics, University of Oslo, Sem Sælands vei 24, 0316, Oslo, Norway.
Division of Technology and Innovation, Oslo University Hospital, 0424, Oslo, Norway.

Søren Erik Pischke (SE)

Department of Emergencies and Critical Care, Oslo University Hospital, 0424, Oslo, Norway.
Institute of Clinical Medicine, University of Oslo, 0318, Oslo, Norway.
Department of Immunology, University of Oslo, 0372, Oslo, Norway.

Runar Strand-Amundsen (R)

Department of Clinical and Biomedical Engineering, Oslo University Hospital, 0424, Oslo, Norway.

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Classifications MeSH

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questionsmedicales.fr › Techniques d'investigation › Perfusion › Utilization of dielectric properties for...
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