The double knockdown of the mitochondrial uncoupling protein isoforms reveals partial redundant roles during Arabidopsis thaliana vegetative and reproductive development.
Arabidopsis thaliana
Double mutant
Mitochondria
Uncoupling protein
Journal
Plant science : an international journal of experimental plant biology
ISSN: 1873-2259
Titre abrégé: Plant Sci
Pays: Ireland
ID NLM: 9882015
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
received:
24
01
2022
revised:
22
06
2022
accepted:
24
06
2022
pubmed:
3
7
2022
medline:
22
7
2022
entrez:
2
7
2022
Statut:
ppublish
Résumé
Mitochondrial uncoupling proteins (UCPs) are specialized proteins capable of dissipating the proton electrochemical gradient generated in respiration independent of ATP synthesis. Three UCP coding genes with distinct expression patterns have been identified in Arabidopsis thaliana (namely UCP1, UCP2 and UCP3). Here, we generated T-DNA double-insertion mutants (ucp1 ucp2, ucp1 ucp3 and ucp2 ucp3) to investigate the functionality of the Arabidopsis UCP isoforms. A strong compensatory effect of the wild-type UCP gene was found in the double-knockdown lines. Higher levels of reactive oxygen species (ROS) were observed in vegetative and reproductive organs of double mutant plants. This exacerbated oxidative stress in plants also increased lipid peroxidation but was not compensated by the activation of the antioxidant system. Alterations in O
Identifiants
pubmed: 35779675
pii: S0168-9452(22)00189-3
doi: 10.1016/j.plantsci.2022.111365
pii:
doi:
Substances chimiques
Ion Channels
0
Mitochondrial Proteins
0
Protein Isoforms
0
Uncoupling Protein 1
0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111365Informations de copyright
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