Sequence, structure, and function of the Dps DNA-binding protein from Deinococcus wulumuqiensis R12.

Bacterial Proteins / metabolism DNA DNA-Binding Proteins / genetics Deinococcus Hydrogen Peroxide
DNA-binding protein Deinococcus wulumuqiensis R12 Oxidative stress Proteomics

Journal

Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812

Informations de publication

Date de publication:
02 Jul 2022
Historique:
received: 05 12 2021
accepted: 21 06 2022
entrez: 2 7 2022
pubmed: 3 7 2022
medline: 7 7 2022
Statut: epublish

Résumé

Deinococcus wulumuqiensis R12, which was isolated from arid irradiated soil in Xinjiang province of China, belongs to a genus that is well-known for its extreme resistance to ionizing radiation and oxidative stress. The DNA-binding protein Dps has been studied for its great contribution to oxidative resistance. To explore the role of Dps in D. wulumuqiensis R12, the Dps sequence and homology-modeled structure were analyzed. In addition, the dps gene was knocked out and proteomics was used to verify the functions of Dps in D. wulumuqiensis R12. Docking data and DNA binding experiments in vitro showed that the R12 Dps protein has a better DNA binding ability than the Dps1 protein from D. radiodurans R1. When the dps gene was deleted in D. wulumuqiensis R12, its resistance to H

Identifiants

DOI: 10.1186/s12934-022-01857-7 PMID: 35780107 PMC: PMC9250271
pubmed: 35780107
doi: 10.1186/s12934-022-01857-7
pii: 10.1186/s12934-022-01857-7
pmc: PMC9250271
doi:

Substances chimiques

Bacterial Proteins 0
DNA-Binding Proteins 0
DNA 9007-49-2
Hydrogen Peroxide BBX060AN9V

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

132

Subventions

Organisme : National Natural Science Foundation of China
ID : 31922070
Organisme : National Natural Science Foundation of China
ID : U2106228
Organisme : National Natural Science Foundation of China
ID : 32060004
Organisme : Postgraduate Research & Practice Innovation Program of Jiangsu Province
ID : KYCX19_0872
Organisme : Fundamental Research Funds for the Central Universities
ID : 0214-14380502

Informations de copyright

© 2022. The Author(s).

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Auteurs

Yao Chen (Y)

College of Food Science and Light Industry, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China.

Zhihan Yang (Z)

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China.

Xue Zhou (X)

College of Food Science and Light Industry, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.

Mengmeng Jin (M)

College of Food Science and Light Industry, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.

Zijie Dai (Z)

College of Food Science and Light Industry, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China.

Dengming Ming (D)

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China.

Zhidong Zhang (Z)

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China. zhangzheedong@sohu.com.
Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences/Xinjiang Key Laboratory of Special Environmental Microbiology, Ürümqi, 830091, Xinjiang, China. zhangzheedong@sohu.com.

Liying Zhu (L)

School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, China. zlyhappy@njtech.edu.cn.

Ling Jiang (L)

College of Food Science and Light Industry, State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing, 211816, China. jiangling@njtech.edu.cn.
ORCID: 0000-0001-6625-5557

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Sequence, structure, and function of the Dps...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Sequence, structure, and function of the Dps...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Sequence, structure, and function of the Dps...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Cocci à Gram positif › Deinococcus › Sequence, structure, and function of the Dps...
questionsmedicales.fr › Composés chimiques organiques › Radicaux libres › Peroxydes › Peroxyde d'hydrogène › Sequence, structure, and function of the Dps...