Nicotinamide Promotes Formation of Retinal Organoids From Human Pluripotent Stem Cells
BMP pathway
induced pluripotent stem cells
neuroectoderm
nicotinamide
retinal organoids
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2022
2022
Historique:
received:
17
02
2022
accepted:
25
05
2022
entrez:
5
7
2022
pubmed:
6
7
2022
medline:
6
7
2022
Statut:
epublish
Résumé
Retinal organoids (ROs) derived from human pluripotent stem cells (hPSCs) recapitulate key features of retinogenesis and provide a promising platform to study retinal development and disease in a human context. Although multiple protocols are currently in use, hPSCs exhibit tremendous variability in differentiation efficiency, with some cell lines consistently yielding few or even no ROs, limiting their utility in research. We report here that early nicotinamide (NAM) treatment significantly improves RO yield across 8 hPSC lines from different donors, including some that would otherwise fail to generate a meaningful number of ROs. NAM treatment promotes neural commitment of hPSCs at the expense of non-neural ectodermal cell fate, which in turn increases eye field progenitor generation. Further analysis suggests that this effect is partially mediated through inhibition of BMP signaling. Our data encourage a broader use of human ROs for disease modeling applications that require the use of multiple patient-specific cell lines.
Identifiants
pubmed: 35783089
doi: 10.3389/fncel.2022.878351
pmc: PMC9247291
doi:
Types de publication
Journal Article
Langues
eng
Pagination
878351Informations de copyright
Copyright © 2022 Regent, Batz, Kelley, Gieser, Swaroop, Chen and Li.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Nature. 2017 Jun 15;546(7658):370-375
pubmed: 28489815
Stem Cells Dev. 2021 Apr;30(8):399-417
pubmed: 33677999
Stem Cell Reports. 2017 Oct 10;9(4):1043-1052
pubmed: 28919261
Nat Commun. 2015 Feb 19;6:6286
pubmed: 25695148
Methods Mol Biol. 2012;884:319-34
pubmed: 22688717
Nat Rev Neurosci. 2002 Apr;3(4):271-80
pubmed: 11967557
Sci Rep. 2016 Aug 23;6:32007
pubmed: 27550649
Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8518-23
pubmed: 24912154
EMBO J. 2004 Feb 25;23(4):844-56
pubmed: 14963489
Cell Stem Cell. 2012 Jun 14;10(6):771-785
pubmed: 22704518
Stem Cells. 2015 Dec;33(12):3504-18
pubmed: 26235913
J Nucleic Acids. 2010 Jul 25;2010:
pubmed: 20725615
Adv Exp Med Biol. 2019;1123:71-94
pubmed: 31016596
Cell Stem Cell. 2015 Jul 2;17(1):101-15
pubmed: 26140606
Cell Stem Cell. 2009 Oct 2;5(4):396-408
pubmed: 19796620
Stem Cell Reports. 2018 Dec 11;11(6):1347-1356
pubmed: 30503259
Stem Cells Transl Med. 2019 Jul;8(7):694-706
pubmed: 30916455
Sci Rep. 2019 Jul 23;9(1):10646
pubmed: 31337830
Stem Cells. 2020 Feb;38(2):195-201
pubmed: 31721366
Dev Neurobiol. 2012 Jul;72(7):1068-84
pubmed: 22489086
Cell. 2020 Sep 17;182(6):1623-1640.e34
pubmed: 32946783
Cell Res. 2012 Nov;22(11):1546-61
pubmed: 22945355
Mol Vis. 2019 Nov 11;25:663-678
pubmed: 31814692
Mol Cell Neurosci. 2020 Sep;107:103523
pubmed: 32634576
Cell Rep. 2017 Jul 11;20(2):384-396
pubmed: 28700940
Neuroreport. 2013 Dec 18;24(18):1041-6
pubmed: 24257250
Stem Cell Reports. 2018 Apr 10;10(4):1267-1281
pubmed: 29526738
Development. 2019 Jan 9;146(1):
pubmed: 30567931
Stem Cells. 2009 Aug;27(8):1772-81
pubmed: 19544437
PLoS One. 2017 Aug 17;12(8):e0183358
pubmed: 28817722
Stem Cells Transl Med. 2013 May;2(5):384-93
pubmed: 23599499
Cytokine Growth Factor Rev. 2005 Jun;16(3):251-63
pubmed: 15871923
Invest Ophthalmol Vis Sci. 2016 Apr 1;57(5):ORSFl1-ORSFl11
pubmed: 27116668
Dev Biol. 2014 May 1;389(1):2-12
pubmed: 24321819
Sci Rep. 2015 Jun 11;5:11319
pubmed: 26066579
Nat Commun. 2014 Jun 10;5:4047
pubmed: 24915161
Stem Cells. 2011 Aug;29(8):1206-18
pubmed: 21678528
Mol Vis. 2020 Mar 03;26:97-105
pubmed: 32174751
Nature. 2010 Sep 16;467(7313):285-90
pubmed: 20644535