Isolation and Characterization of Engineered Nucleoside Deoxyribosyltransferase with Enhanced Activity Toward 2'-Fluoro-2'-Deoxynucleoside.
2’-fluoro-2’-deoxynucleoside
Nucleoside deoxyribosyltransferase
enzyme kinetics
error-prone PCR
substrate specificity
Journal
Journal of microbiology and biotechnology
ISSN: 1738-8872
Titre abrégé: J Microbiol Biotechnol
Pays: Korea (South)
ID NLM: 9431852
Informations de publication
Date de publication:
28 Aug 2022
28 Aug 2022
Historique:
received:
28
04
2022
revised:
08
06
2022
accepted:
11
06
2022
pubmed:
7
7
2022
medline:
31
8
2022
entrez:
6
7
2022
Statut:
ppublish
Résumé
Nucleoside deoxyribosyltransferase (NDT) is an enzyme that replaces the purine or pyrimidine base of 2'-deoxyribonucleoside. This enzyme is generally used in the nucleotide salvage pathway in vivo and synthesizes many nucleoside analogs in vitro for various biotechnological purposes. Since NDT is known to exhibit relatively low reactivity toward nucleoside analogs such as 2'-fluoro-2'-deoxynucleoside, it is necessary to develop an enhanced NDT mutant enzyme suitable for nucleoside analogs. In this study, molecular evolution strategy via error-prone PCR was performed with ndt gene derived from
Identifiants
pubmed: 35791073
pii: jmb.2204.04041
doi: 10.4014/jmb.2204.04041
pmc: PMC9628941
doi:
Substances chimiques
nucleoside deoxyribosyltransferase
EC 2.4.2.6
Nucleosides
0
Pentosyltransferases
EC 2.4.2.-
2'fluoro-2'-deoxyuridine
784-71-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1041-1046Références
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